Baseclick DEAE Dextran 说明书

  

上海金畔生物为Baseclick代理,Baseclick由BASF VC和慕尼黑LMU于2008年创立,自此为的公司,实验室和科研机构提供高品质的生命科学产品和试剂。Baseclick是在生命科学创新解决方案方面的专家。Baseclick提供基于点击化学的产品,用于核酸的,灵敏和模块化标记。

 

将核酸(DNA / RNA)输送到细胞中对于基因表达和沉默实验至关重要,并且需要适当的转染策略。已经确定了几种成功转染的方法。一种方法是基于阳离子聚合物与带负电荷的DNA / RNA分子的络合。通过与细胞膜粘附并随后内吞,复合物被细胞摄取。可以以这种方式使用的试剂是DEAE-葡聚糖或聚乙烯亚胺。另一种策略是受体介导的内化的原理。这样的受体配体如叶酸-PEG3-叠氮化物显示出突出的转染特性。选择正确的转染试剂非常重要,应考虑每种细胞类型。

 

Baseclick  DEAE Dextran 说明书:

 

品名:DEAE Dextran 

BCFA-213-10   10 g

BCFA-213-25   25 g

 

文本:

CAS号:9013-34-7

图片:

 

名称:

二乙氨基乙基葡聚糖

储存条件:
RT

外观:
白色至奶油色粉末

保质期:
12个月

 

Baseclick产品列表:

 

货号

品名

规格

目录价

品牌

BCA-01-100

5`-Hexynyl-CEP

100mg

1500

baseclick

BCA-01-250

5`-Hexynyl-CEP

250 mg

4000

baseclick

BCA-01-1g

5`-Hexynyl-CEP

1 g

13600

baseclick

BCA-02-100

3`-Alkyne-Modifier Serinol CPG

100 mg

4200

baseclick

BCA-02-1g

3`-Alkyne-Modifier Serinol CPG

1 g

17100

baseclick

BCA-03-250

C8-Alkyne-dU-CEP

250 mg

4200

baseclick

BCA-03-1g

C8-Alkyne-dU-CEP

1 g

12600

baseclick

BCA-03-5g

C8-Alkyne-dU-CEP

5 g

50000

baseclick

BCA-04-250

C8-TMS-dC-CEP

250 mg

3000

baseclick

BCA-04-1g

C8-TMS-dC-CEP

1 g

12000

baseclick

BCA-04-5g

C8-TMS-dC-CEP

5 g

60000

baseclick

BCA-05-250

5-Propargyloxy-dU-CEP

250 mg

22000

baseclick

BCA-05-1g

5-Propargyloxy-dU-CEP

1 g

63000

baseclick

BCA-07-250

C8-Alkyne-dC-CEP

250 mg

4800

baseclick

BCA-07-1g

C8-Alkyne-dC-CEP

1 g

15600

baseclick

BCA-07-5g

C8-Alkyne-dC-CEP

5 g

74000

baseclick

BCA-08-250

C8-TIPS-dC-CEP

250 mg

3000

baseclick

BCA-08-1g

C8-TIPS-dC-CEP

1 g

12000

baseclick

BCA-08-5g

C8-TIPS-dC-CEP

5 g

60000

baseclick

BCA-09-100

5-Ethynyl-dU-CEP

100 µmol

5000

baseclick

BCA-09-250

5-Ethynyl-dU-CEP

250 mg

11600

baseclick

BCA-09-1g

5-Ethynyl-dU-CEP

1 g

36000

baseclick

BCA-09-5g

5-Ethynyl-dU-CEP

5 g

144000

baseclick

BCA-014-250

5-Ethynyl uridine CEP

250 mg

24000

baseclick

BCA-25-100

TIPS-5-Ethynyl-dU-CEP

100 mg

6400

baseclick

BCA-25-250

TIPS-5-Ethynyl-dU-CEP

250 mg

13000

baseclick

BCA-25-1g

TIPS-5-Ethynyl-dU-CEP

1 g

44000

baseclick

BCA-25-5g

TIPS-5-Ethynyl-dU-CEP

5 g

220000

baseclick

BCA-20-1g

Abasic-Alkyne-CEP

1 g

15800

baseclick

BCT-05-S

C8-Alkyne-dUTP

0.5 µmol

1500

baseclick

BCT-05-M

C8-Alkyne-dUTP

2.5 µmol

2400

baseclick

BCT-05-L

C8-Alkyne-dUTP

5.0 µmol

4800

baseclick

BCT-06-S

C8-Alkyne-dCTP

0.5 µmol

1500

baseclick

BCT-06-M

C8-Alkyne-dCTP

2.5 µmol

2400

baseclick

BCT-06-L

C8-Alkyne-dCTP

5.0 µmol

4800

baseclick

BCT-07-S

5-Ethynyl-UTP

0.5 µmol

3000

baseclick

BCT-07-M

5-Ethynyl-UTP

2.5 µmol

11600

baseclick

BCT-07-L

5-Ethynyl-UTP

5.0 µmol

15000

baseclick

BCT-08-S

5-Ethynyl-dUTP

0.5 µmol

1500

baseclick

BCT-08-M

5-Ethynyl-dUTP

2.5 µmol

2800

baseclick

BCT-08-L

5-Ethynyl-dUTP

5.0 µmol

4800

baseclick

BCT-09-S

AA-dCTP (Aminoallyl-dCTP)

1.0 µmol

4200

baseclick

BCT-09-M

AA-dCTP (Aminoallyl-dCTP)

5.0 µmol

11600

baseclick

BCT-09-L

AA-dCTP (Aminoallyl-dCTP)

10.0 µmol

18000

baseclick

BCT-10-S

AA-dUTP (Aminoallyl-dUPT)

1.0 µmol

4200

baseclick

BCT-10-M

AA-dUTP (Aminoallyl-dUPT)

5.0 µmol

11600

baseclick

BCT-10-L

AA-dUTP (Aminoallyl-dUPT)

10.0 µmol

18000

baseclick

BCT-11-S

AA-CTP (Aminoallyl-CTP)

1.0 µmol

4200

baseclick

BCT-11-M

AA-CTP (Aminoallyl-CTP)

5.0 µmol

11600

baseclick

BCT-11-L

AA-CTP (Aminoallyl-CTP)

10.0 µmol

18000

baseclick

BCT-12-S

AA-UTP (Aminoallyl-UTP)

1.0 µmol

4200

baseclick

BCT-12-M

AA-UTP (Aminoallyl-UTP)

5.0 µmol

11600

baseclick

BCT-12-L

AA-UTP (Aminoallyl-UTP)

10.0 µmol

18000

baseclick

BCT-13-S

Alpha-thio-dATP

1.0 µmol

3800

baseclick

BCT-13-M

Alpha-thio-dATP

5.0 µmol

8800

baseclick

BCT-13-L

Alpha-thio-dATP

10.0 µmol

13200

baseclick

BCT-14-S

Alpha-thio-dCTP

1.0 µmol

3800

baseclick

BCT-14-M

Alpha-thio-dCTP

5.0 µmol

8800

baseclick

BCT-14-L

Alpha-thio-dCTP

10.0 µmol

13200

baseclick

BCT-15-S

Alpha-thio-dGTP

1.0 µmol

3800

baseclick

BCT-15-M

Alpha-thio-dGTP

5.0 µmol

8800

baseclick

BCT-15-L

Alpha-thio-dGTP

10.0 µmol

13200

baseclick

BCT-16-S

Alpha-thio-dTTP

1.0 µmol

3800

baseclick

BCT-16-M

Alpha-thio-dTTP

5.0 µmol

8800

baseclick

BCT-16-L

Alpha-thio-dTTP

10.0 µmol

13200

baseclick

BCT-17-S

Biotin-5-dCTP

0.25 µmol

5800

baseclick

BCT-17-M

Biotin-5-dCTP

1.0 µmol

14600

baseclick

BCT-18-S

Biotin-11-dCTP

0.25 µmol

5800

baseclick

BCT-18-M

Biotin-11-dCTP

1.0 µmol

14600

baseclick

BCT-19-S

Biotin-16-dCTP

0.25 µmol

5800

baseclick

BCT-19-M

Biotin-16-dCTP

1.0 µmol

14600

baseclick

BCT-20-S

Biotin-5-dUTP

0.25 µmol

5800

baseclick

BCT-20-M

Biotin-5-dUTP

1.0 µmol

14600

baseclick

BCT-21-S

Alkyne-dATP

0.5 µmol

1500

baseclick

BCT-21-M

Alkyne-dATP

2.5 µmol

2600

baseclick

BCT-21-L

Alkyne-dATP

5.0 µmol

4800

baseclick

BCT-22-S

Alkyne-ATP

0.5 µmol

1800

baseclick

BCT-22-M

Alkyne-ATP

2.5 µmol

5200

baseclick

BCT-22-L

Alkyne-ATP

5.0 µmol

8000

baseclick

BCFA-002-1

Dabcyl Azide

1 mg

900

baseclick

BCFA-002-5

Dabcyl Azide

5 mg

1600

baseclick

BCFA-002-10

Dabcyl Azide

10 mg

2500

baseclick

BCFA-002-100

Dabcyl Azide

100 mg

10000

baseclick

BCFA-003-1

Biotin Azide

1 mg

1300

baseclick

BCFA-003-5

Biotin Azide

5 mg

1600

baseclick

BCFA-003-10

Biotin Azide

10 mg

2500

baseclick

BCFA-003-50

Biotin Azide

50 mg

6400

baseclick

BCFA-003-100

Biotin Azide

100 mg

10000

baseclick

BCFA-009-1

Dabsyl Azide

1 mg

900

baseclick

BCFA-009-5

Dabsyl Azide

5 mg

1600

baseclick

BCFA-019-1

Ferrocene Azide

1 mg

900

baseclick

BCFA-019-5

Ferrocene Azide

5 mg

2600

baseclick

BCFA-021-1

Biotin-PEG3-Azide

1 mg

3000

baseclick

BCFA-021-5

Biotin-PEG3-Azide

5 mg

4200

baseclick

BCFA-048-1

Desthiobiotin-PEG3-Azide

1 mg

2200

baseclick

BCFA-048-5

Desthiobiotin-PEG3-Azide

5 mg

8400

baseclick

BCAL-040-1

Biotin-PEG4-Alkyne

1 mg

2300

baseclick

BCAL-040-5

Biotin-PEG4-Alkyne

5 mg

8600

baseclick

BCFA-001-1

Fluorescein Azide (6-FAM)

1 mg

900

baseclick

BCFA-001-5

Fluorescein Azide (6-FAM)

5 mg

1600

baseclick

BCFA-001-10

Fluorescein Azide (6-FAM)

10 mg

2500

baseclick

BCFA-001-50

Fluorescein Azide (6-FAM)

50 mg

6400

baseclick

BCFA-001-100

Fluorescein Azide (6-FAM)

100 mg

10000

baseclick

BCFA-008-1

5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

1 mg

1200

baseclick

BCFA-008-5

5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

5 mg

1600

baseclick

BCFA-008-10

5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

10 mg

2500

baseclick

BCFA-008-50

5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

50 mg

6400

baseclick

BCFA-008-100

5-Carboxytetramethylrhodamine Azide (5-TAMRA-Azide)

100 mg

10000

baseclick

BCFA-016-1

Dansyl Azide

1 mg

900

baseclick

BCFA-016-5

Dansyl Azide

5 mg

1600

baseclick

BCFA-017-1

Pyrene Azide

1 mg

900

baseclick

BCFA-017-5

Pyrene Azide

5 mg

1600

baseclick

BCFA-018-1

5-Sulforhodamine B Azide

1 mg

900

baseclick

BCFA-018-5

5-Sulforhodamine B Azide

5 mg

1600

baseclick

BCFA-036-1

Dansyl-PEG3-Azide (water soluble)

1 mg

1800

baseclick

BCFA-036-5

Dansyl-PEG3-Azide (water soluble)

5 mg

7200

baseclick

BCFA-037-1

5-Carboxytetramethylrhodamine-PEG3-Azide(5-TAMRA-PEG3-Azide)

1 mg

900

baseclick

BCFA-037-5

5-Carboxytetramethylrhodamine-PEG3-Azide(5-TAMRA-PEG3-Azide)

5 mg

1600

baseclick

BCFA-037-10

5-Carboxytetramethylrhodamine-PEG3-Azide(5-TAMRA-PEG3-Azide)

10 mg

2500

baseclick

BCFA-037-50

5-Carboxytetramethylrhodamine-PEG3-Azide(5-TAMRA-PEG3-Azide)

50 mg

6400

baseclick

BCFA-037-100

5-Carboxytetramethylrhodamine-PEG3-Azide(5-TAMRA-PEG3-Azide)

100 mg

10000

baseclick

BCFA-044-1

5/6-Sulforhodamine 101-PEG3-Azide(Texas RedR Alternative)

1 mg

1600

baseclick

BCFA-044-5

5/6-Sulforhodamine 101-PEG3-Azide(Texas RedR Alternative)

5 mg

4800

baseclick

BCFA-047-1

3-Azido-7-hydroxycoumarin

1 mg

1800

baseclick

BCFA-047-5

3-Azido-7-hydroxycoumarin

5 mg

6300

baseclick

BCFA-049-1

6-FAM-PEG3-Azide

1 mg

2800

baseclick

BCFA-049-5

6-FAM-PEG3-Azide

5 mg

11000

baseclick

BCFA-080-1

Cyanine 3 Azide

1 mg

2800

baseclick

BCFA-080-5

Cyanine 3 Azide

5 mg

11000

baseclick

BCFA-081-1

Cyanine 3.5 Azide

1 mg

2800

baseclick

BCFA-081-5

Cyanine 3.5 Azide

5 mg

11000

baseclick

BCFA-082-1

Cyanine 5 Azide

1 mg

2800

baseclick

BCFA-082-5

Cyanine 5 Azide

5 mg

11000

baseclick

BCFA-083-1

Cyanine 5.5 Azide

1 mg

2800

baseclick

BCFA-083-5

Cyanine 5.5 Azide

5 mg

11000

baseclick

BCFA-011-1

EterneonTM-350/455 Azide

1 mg

800

baseclick

BCFA-011-5

EterneonTM-350/455 Azide

5 mg

3080

baseclick

BCFA-011-10

EterneonTM-350/455 Azide

10 mg

6160

baseclick

BCFA-013-1

EterneonTM-393/523 Azide

1 mg

800

baseclick

BCFA-013-5

EterneonTM-393/523 Azide

5 mg

3080

baseclick

BCFA-013-10

EterneonTM-393/523 Azide

10 mg

6160

baseclick

BCFA-014-1

EterneonTM-394/507 Azide

1 mg

800

baseclick

BCFA-014-5

EterneonTM-394/507 Azide

5 mg

3080

baseclick

BCFA-014-10

EterneonTM-394/507 Azide

10 mg

6160

baseclick

BCFA-031-1

EterneonTM-350/455 NHS

1 mg

800

baseclick

BCFA-031-5

EterneonTM-350/455 NHS

5 mg

3080

baseclick

BCFA-031-10

EterneonTM-350/455 NHS

10 mg

6160

baseclick

BCFA-033-1

EterneonTM-393/523 NHS

1 mg

800

baseclick

BCFA-033-5

EterneonTM-393/523 NHS

5 mg

3080

baseclick

BCFA-033-10

EterneonTM-393/523 NHS

10 mg

6160

baseclick

BCFA-034-1

EterneonTM-394/507 NHS

1 mg

800

baseclick

BCFA-034-5

EterneonTM-394/507 NHS

5 mg

3080

baseclick

BCFA-034-10

EterneonTM-394/507 NHS

10 mg

6160

baseclick

BCFA-201-1

Eterneon-Red 645 Azide

1 mg

2400

baseclick

BCFA-201-5

Eterneon-Red 645 Azide

5 mg

10000

baseclick

BCFA-203-1

Eterneon-Yellow 530 Azide

1 mg

2800

baseclick

BCFA-203-5

Eterneon-Yellow 530 Azide

5 mg

10000

baseclick

BCFA-206-1

Eterneon-Orange 570 Azide

1 mg

3400

baseclick

BCFA-206-5

Eterneon-Orange 570 Azide

5 mg

11000

baseclick

BCFA-209-1

Eterneon-Red 630 Azide

1 mg

3400

baseclick

BCFA-209-5

Eterneon-Red 630 Azide

5 mg

11000

baseclick

BCFA-219-1

Eterneon²488 Azide (FITC alternative)

1 mg

4000

baseclick

BCFA-219-5

Eterneon²488 Azide (FITC alternative)

5 mg

7600

baseclick

BCFA-220-1

Eterneon²550 Azide (Cy3TM alternative)

1 mg

4200

baseclick

BCFA-220-5

Eterneon²550 Azide (Cy3TM alternative)

5 mg

9000

baseclick

BCFA-221-1

Eterneon²555 Azide (TAMRA alternative)

1 mg

4000

baseclick

BCFA-221-5

Eterneon²555 Azide (TAMRA alternative)

5 mg

7600

baseclick

BCFA-222-1

Eterneon²647 Azide (Cy5TM alternative)

1 mg

4200

baseclick

BCFA-222-5

Eterneon²647 Azide (Cy5TM alternative)

5 mg

9000

baseclick

BCFA-223-1

Eterneon²678 Azide (Cy5.5TM alternative)

1 mg

4200

baseclick

BCFA-223-5

Eterneon²678 Azide (Cy5.5TM alternative)

5 mg

9000

baseclick

BCFA-224-1

Eterneon²754 Azide (Cy7TM alternative)

1 mg

4200

baseclick

BCFA-224-5

Eterneon²754 Azide (Cy7TM alternative)

5 mg

9000

baseclick

BCFA-225-5

Eterneon²Biotin Azide (Biotin analogue)

5 mg

4000

baseclick

BCFA-225-25

Eterneon²Biotin Azide (Biotin analogue)

25 mg

7600

baseclick

BCAL-001-1

5/6-Carboxyrhodamine 110-PEG4-Alkyne

1 mg

2000

baseclick

BCAL-001-5

5/6-Carboxyrhodamine 110-PEG4-Alkyne

5 mg

7600

baseclick

BCAL-003-1

5/6-Carboxytetramethylrhodamine-PEG4-Alkyne(5/6-TAMRA-PEG4-Alkyne)

1 mg

2000

baseclick

BCAL-003-5

5/6-Carboxytetramethylrhodamine-PEG4-Alkyne(5/6-TAMRA-PEG4-Alkyne)

5 mg

7600

baseclick

BCAL-010-1

Cyanine3-Alkyne

1 mg

3600

baseclick

BCAL-010-5

Cyanine3-Alkyne

5 mg

9000

baseclick

BCAL-011-1

Cyanine5-Alkyne

1 mg

3600

baseclick

BCAL-011-5

Cyanine5-Alkyne

5 mg

9000

baseclick

BCAL-012-1

Cyanine 5.5-Alkyne

1 mg

3600

baseclick

BCAL-012-5

Cyanine 5.5-Alkyne

5 mg

9000

baseclick

BCAL-013-1

Cyanine7-Alkyne

1 mg

3600

baseclick

BCAL-013-5

Cyanine7-Alkyne

5 mg

9000

baseclick

BCAL-014-5

Alkyne Stearic Acid

5 mg

3000

baseclick

BCAL-014-25

Alkyne Stearic Acid

25 mg

6400

baseclick

BCAL-015-5

Alkyne Palmitic Acid

5 mg

3000

baseclick

BCAL-015-25

Alkyne Palmitic Acid

25 mg

6400

baseclick

BCAL-016-5

Alkyne Myristic Acid

5 mg

3000

baseclick

BCAL-016-25

Alkyne Myristic Acid

25 mg

6400

baseclick

BCMI-001-5

CuBr (baseclick grade – organic solvents)

5 mg

240

baseclick

BCMI-001-10

CuBr (baseclick grade – organic solvents)

10 x 5 mg

1420

baseclick

BCMI-002-5

TBTA-Ligand (baseclick grade – organic solvents)

5 mg

650

baseclick

BCMI-002-10

TBTA-Ligand (baseclick grade – organic solvents)

10 mg

900

baseclick

BCMI-002-50

TBTA-Ligand (baseclick grade – organic solvents)

50 mg

1700

baseclick

BCMI-002-100

TBTA-Ligand (baseclick grade – organic solvents)

100 mg

2900

baseclick

BCMI-003-1

Click-Solution (baseclick grade – DMSO/t-Butanol)

1.0 ml

240

baseclick

BCMI-003-10

Click-Solution (baseclick grade – DMSO/t-Butanol)

10 x 1.0 ml

1284

baseclick

BCMI-004-10

CuSO4 (baseclick grade – water soluble)

10 mg

240

baseclick

BCMI-004-50

CuSO4 (baseclick grade – water soluble)

5 x 10 mg

700

baseclick

BCMI-005-10

Na-Ascorbate (baseclick grade – water soluble)

10 mg

240

baseclick

BCMI-005-50

Na-Ascorbate (baseclick grade – water soluble)

5 x 10 mg

720

baseclick

BCMI-006-5

THPTA-Ligand (water soluble – water soluble)

5 mg

650

baseclick

BCMI-006-10

THPTA-Ligand (water soluble – water soluble)

10 mg

900

baseclick

BCMI-006-50

THPTA-Ligand (water soluble – water soluble)

50 mg

1700

baseclick

BCMI-006-100

THPTA-Ligand (water soluble – water soluble)

100 mg

2900

baseclick

BCMI-007-5

BTTAA-Ligand

5 mg

1040

baseclick

BCMI-007-10

BTTAA-Ligand

10 mg

1760

baseclick

BCMI-007-50

BTTAA-Ligand

50 mg

3400

baseclick

BCMI-007-100

BTTAA-Ligand

100 mg

5500

baseclick

BCL-001-5

NHS-PEG4-Azide

5 mg

1200

baseclick

BCL-001-10

NHS-PEG4-Azide

10 mg

1800

baseclick

BCL-002-5

PEG24-Azide

5 mg

900

baseclick

BCL-002-10

PEG24-Azide

10 mg

1520

baseclick

BCL-003-5

PEG8-Azide

5 mg

1200

baseclick

BCL-003-10

PEG8-Azide

10 mg

1800

baseclick

BCL-004-5

HO-PEG2-Azide

5 mg

800

baseclick

BCL-004-10

HO-PEG2-Azide

10 mg

1300

baseclick

BCL-005-5

H2N-PEG8-Azide

5 mg

800

baseclick

BCL-005-10

H2N-PEG8-Azide

10 mg

1300

baseclick

BCL-007-5

4-Azidoaniline Hydrochlorid

5 mg

660

baseclick

BCL-007-10

4-Azidoaniline Hydrochlorid

10 mg

900

baseclick

BCL-008-5

4-Azidobenzoic acid

5 mg

660

baseclick

BCL-008-10

4-Azidobenzoic acid

10 mg

900

baseclick

BCL-009-5

5-Azidopentanoic acid

5 mg

680

baseclick

BCL-009-10

5-Azidopentanoic acid

10 mg

900

baseclick

BCL-010-5

Mal-PEG3-Azide (2 component kit)

25 mg

2600

baseclick

BCL-011-5

H2N-PEG3-Azide

5 mg

800

baseclick

BCL-011-10

H2N-PEG3-Azide

10 mg

1300

baseclick

BCL-014-5

NHS-C3-Azide

5 mg

800

baseclick

BCL-014-10

NHS-C3-Azide

10 mg

1300

baseclick

BCL-032-5

NHS-PEG8-Azide

5 mg

1100

baseclick

BCL-032-10

NHS-PEG8-Azide

10 mg

1600

baseclick

BCL-033-5

NHS-PEG12-Azide

5 mg

1100

baseclick

BCL-033-10

NHS-PEG12-Azide

10 mg

1600

baseclick

BCL-034-5

Aminooxy-PEG3-Azide

5 mg

1700

baseclick

BCL-034-10

Aminooxy-PEG3-Azide

10 mg

2600

baseclick

BCL-091-5

H2N-PEG4-Alkyne

5 mg

1300

baseclick

BCL-091-10

H2N-PEG4-Alkyne

10 mg

2300

baseclick

BCL-093-5

Mal-PEG4-Alkyne

5 mg

1300

baseclick

BCL-093-10

Mal-PEG4-Alkyne

10 mg

2300

baseclick

BCL-095-5

NHS-PEG5-Alkyne

5 mg

1200

baseclick

BCL-095-10

NHS-PEG5-Alkyne

10 mg

2000

baseclick

BCL-096-5

HOOC-PEG5-Alkyne

5 mg

1300

baseclick

BCL-096-10

HOOC-PEG5-Alkyne

10 mg

2300

baseclick

BCN-001-5

5-Ethynyl-dU (EdU – bio grade)

5 mg

500

baseclick

BCN-001-10

5-Ethynyl-dU (EdU – bio grade)

10 mg

800

baseclick

BCN-001-50

5-Ethynyl-dU (EdU – bio grade)

50 mg

2000

baseclick

BCN-001-100

5-Ethynyl-dU (EdU – bio grade)

100 mg

3400

baseclick

BCN-001-500

5-Ethynyl-dU (EdU – bio grade)

500 mg

14000

baseclick

BCN-001-1g

5-Ethynyl-dU (EdU – bio grade)

1 g

22000

baseclick

BCN-003-5

5-Ethynyl uridine (EU – bio grade)

5 mg

1100

baseclick

BCN-003-10

5-Ethynyl uridine (EU – bio grade)

10 mg

1800

baseclick

BCAA-001-5

4-Azido-L-phenylalanine

5 mg

800

baseclick

BCAA-001-10

4-Azido-L-phenylalanine

10 mg

1300

baseclick

BCAA-001-100

4-Azido-L-phenylalanine

100 mg

4300

baseclick

BCAA-001-500

4-Azido-L-phenylalanine

500 mg

11000

baseclick

BCAA-002-5

4-Azido-D-phenylalanine

5 mg

800

baseclick

BCAA-002-10

4-Azido-D-phenylalanine

10 mg

1300

baseclick

BCAA-002-100

4-Azido-D-phenylalanine

100 mg

4300

baseclick

BCAA-002-500

4-Azido-D-phenylalanine

500 mg

11000

baseclick

BCAA-003-5

3-Azido-L-alanine HCl

5 mg

800

baseclick

BCAA-003-10

3-Azido-L-alanine HCl

10 mg

1300

baseclick

BCAA-003-100

3-Azido-L-alanine HCl

100 mg

4300

baseclick

BCAA-003-500

3-Azido-L-alanine HCl

500 mg

11000

baseclick

BCAA-004-5

3-Azido-D-alanine HCl

5 mg

800

baseclick

BCAA-004-10

3-Azido-D-alanine HCl

10 mg

1300

baseclick

BCAA-004-100

3-Azido-D-alanine HCl

100 mg

4300

baseclick

BCAA-004-500

3-Azido-D-alanine HCl

500 mg

11000

baseclick

BCAA-005-5

4-Azido-L-homoalanine HCl

5 mg

800

baseclick

BCAA-005-10

4-Azido-L-homoalanine HCl

10 mg

1300

baseclick

BCAA-005-100

4-Azido-L-homoalanine HCl

100 mg

4300

baseclick

BCAA-005-500

4-Azido-L-homoalanine HCl

500 mg

11000

baseclick

BCAA-006-5

4-Azido-D-homoalanine HCl

5 mg

800

baseclick

BCAA-006-10

4-Azido-D-homoalanine HCl

10 mg

1300

baseclick

BCAA-006-100

4-Azido-D-homoalanine HCl

100 mg

4300

baseclick

BCAA-006-500

4-Azido-D-homoalanine HCl

500 mg

11000

baseclick

BCAA-007-5

5-Azido-L-ornithine HCl

5 mg

800

baseclick

BCAA-007-10

5-Azido-L-ornithine HCl

10 mg

1300

baseclick

BCAA-007-100

5-Azido-L-ornithine HCl

100 mg

4300

baseclick

BCAA-007-500

5-Azido-L-ornithine HCl

500 mg

11000

baseclick

BCAA-008-5

5-Azido-D-ornithine HCl

5 mg

800

baseclick

BCAA-008-10

5-Azido-D-ornithine HCl

10 mg

1300

baseclick

BCAA-008-100

5-Azido-D-ornithine HCl

100 mg

4300

baseclick

BCAA-008-500

5-Azido-D-ornithine HCl

500 mg

11000

baseclick

BCAA-009-5

6-Azido-L-lysine HCl

5 mg

800

baseclick

BCAA-009-10

6-Azido-L-lysine HCl

10 mg

1300

baseclick

BCAA-009-100

6-Azido-L-lysine HCl

100 mg

4300

baseclick

BCAA-009-500

6-Azido-L-lysine HCl

500 mg

11000

baseclick

BCAA-010-5

6-Azido-D-lysine HCl

5 mg

800

baseclick

BCAA-010-10

6-Azido-D-lysine HCl

10 mg

1300

baseclick

BCAA-010-100

6-Azido-D-lysine HCl

100 mg

4300

baseclick

BCAA-010-500

6-Azido-D-lysine HCl

500 mg

11000

baseclick

BCK-001

BaseClickTM-Kit Fluorescein

1 Kit

5200

baseclick

BCK-001-5

BaseClickTM-Kit Fluorescein

5 Kits

26000

baseclick

BCK-002

BaseClickTM-Kit Biotin

1 Kit

4900

baseclick

BCK-002-5

BaseClickTM-Kit Biotin

5 Kits

24500

baseclick

BCK-003

BaseClickTM-Kit TAMRA

1 Kit

5600

baseclick

BCK-003-5

BaseClickTM-Kit TAMRA

5 Kits

28000

baseclick

BCK-004

BaseClickTM-Kit Dabcyl

1 Kit

5000

baseclick

BCK-004-5

BaseClickTM-Kit Dabcyl

5 Kits

25000

baseclick

BCK-006

BaseClickTM-Kit EterneonTM 350/455

1 Kit

3800

baseclick

BCK-006-5

BaseClickTM-Kit EterneonTM 350/455

5 Kits

19000

baseclick

BCK-008

BaseClickTM-Kit EterneonTM 393/523

1 Kit

3800

baseclick

BCK-008-5

BaseClickTM-Kit EterneonTM 393/523

5 Kits

19000

baseclick

BCK-009

BaseClickTM-Kit EterneonTM 394/507

1 Kit

3800

baseclick

BCK-009-5

BaseClickTM-Kit EterneonTM 394/507

5 Kits

19000

baseclick

BCK-OligoS

Oligo-Click-S-Basic

1 Kit

1200

baseclick

BCK-OligoM

Oligo-Click-M-Basic

1 Kit

1500

baseclick

BCK-OligoS-R

Oligo-Click-S-Reload

1 Kit

1200

baseclick

BCK-OligoM-R

Oligo-Click-M-Reload

1 Kit

1500

baseclick

BCK-OligoS488

Oligo-Click-S-488

1 Kit

2800

baseclick

BCK-OligoM488

Oligo-Click-M-488

1 Kit

2900

baseclick

BCK-OligoS555

Oligo-Click-S-555

1 Kit

3200

baseclick

BCK-OligoM555

Oligo-Click-M-555

1 Kit

3300

baseclick

BCK-OligoSBiotin

Oligo-Click-SBiotin

1 Kit

2300

baseclick

BCK-OligoMBiotin

Oligo-Click-MBiotin

1 Kit

2400

baseclick

BCK-PCR488-1

PCR-Click 488

1 Kit

4300

baseclick

BCK-PCR555-1

PCR-Click 555

1 Kit

4300

baseclick

BCK-PCR594-1

PCR-Click 594

1 Kit

4300

baseclick

BCK-PCR647-1

PCR-Click 647

1 Kit

4300

baseclick

BCK-EdU488-1

EdU-Click 488

1 Kit

7700

baseclick

BCK-EdU555-1

EdU-Click 555

1 Kit

7700

baseclick

BCK-EdU594-1

EdU-Click 594

1 Kit

7700

baseclick

BCK-EdU647-1

EdU-Click 647

1 Kit

7700

baseclick

BCK-FC488-50

EdU Flow Cytometry Kit 488

1 Kit

7900

baseclick

BCK-FC555-50

EdU Flow Cytometry Kit 555

1 Kit

7900

baseclick

BCK-FC594-50

EdU Flow Cytometry Kit 594

1 Kit

7900

baseclick

BCK-FC647-50

EdU Flow Cytometry Kit 647

1 Kit

7900

baseclick

BCK-FC488-100

EdU Flow Cytometry Kit 488

1 Kit

12500

baseclick

BCK-FC555-100

EdU Flow Cytometry Kit 555

1 Kit

12500

baseclick

BCK-FC594-100

EdU Flow Cytometry Kit 594

1 Kit

12500

baseclick

BCK-FC647-100

EdU Flow Cytometry Kit 647

1 Kit

12500

baseclick

BCK-HTS488-2

EdU HTS Kit 488 (200 reactions or2x96 well plates)

1 Kit

4400

baseclick

BCK-HTS488-4

EdU HTS Kit 488 (400 reactions or4x96 well plates)

1 Kit

6900

baseclick

BCK-HTS488-20

EdU HTS Kit 488 (2000 reactions or 20×96 well plates)

1 Kit

23000

baseclick

BCK-HTS555-2

EdU HTS Kit 555 (200 reactions or2x96 well plates)

1 Kit

4400

baseclick

BCK-HTS555-4

EdU HTS Kit 555 (400 reactions or4x96 well plates)

1 Kit

6900

baseclick

BCK-HTS555-20

EdU HTS Kit 555 (2000 reactions or 20×96 well plates)

1 Kit

23000

baseclick

BCK-HTS594-2

EdU HTS Kit 594 (200 reactions or2x96 well plates)

1 Kit

4400

baseclick

BCK-HTS594-4

EdU HTS Kit 594 (400 reactions or4x96 well plates)

1 Kit

6900

baseclick

BCK-HTS594-20

EdU HTS Kit 594 (2000 reactions or 20×96 well plates)

1 Kit

23000

baseclick

BCK488-IV-IM-S

EdU Click 488 Kit + 50mg EdU

1 Kit

8720

baseclick

BCK555-IV-IM-S

EdU Click 555 Kit + 50mg EdU

1 Kit

8720

baseclick

BCK594-IV-IM-S

EdU Click 594 Kit + 50mg EdU

1 Kit

8720

baseclick

BCK647-IV-IM-S

EdU Click 647 Kit + 50mg EdU

1 Kit

8720

baseclick

BCK488-IV-IM-M

EdU Click 488 Kit + 500mg EdU

1 Kit

18320

baseclick

BCK555-IV-IM-M

EdU Click 555 Kit + 500mg EdU

1 Kit

18320

baseclick

BCK594-IV-IM-M

EdU Click 594 Kit + 500mg EdU

1 Kit

18320

baseclick

BCK647-IV-IM-M

EdU Click 647 Kit + 500mg EdU

1 Kit

18320

baseclick

BCK488-IV-IM-L

EdU Click 488 Kit + 1000mg EdU

1 Kit

26720

baseclick

BCK555-IV-IM-L

EdU Click 555 Kit + 1000mg EdU

1 Kit

26720

baseclick

BCK594-IV-IM-L

EdU Click 594 Kit + 1000mg EdU

1 Kit

26720

baseclick

BCK647-IV-IM-L

EdU Click 647 Kit + 1000mg EdU

1 Kit

26720

baseclick

BCK488-IV-FC-S

EdU Flow Cytometry488-50 Kit+ 50mg EdU

1 Kit

8900

baseclick

BCK555-IV-FC-S

EdU Flow Cytometry555-50 Kit+ 50mg EdU

1 Kit

8900

baseclick

BCK594-IV-FC-S

EdU Flow Cytometry594-50 Kit+ 50mg EdU

1 Kit

8900

baseclick

BCK647-IV-FC-S

EdU Flow Cytometry647-50 Kit+ 50mg EdU

1 Kit

8900

baseclick

BCK488-IV-FC-M

EdU Flow Cytometry488- 50 Kit+ 500mg EdU

1 Kit

19700

baseclick

BCK555-IV- FC -M

EdU Flow Cytometry555- 50 Kit+ 500mg EdU

1 Kit

19700

baseclick

BCK594-IV- FC -M

EdU Flow Cytometry594- 50 Kit+ 500mg EdU

1 Kit

19700

baseclick

BCK647-IV- FC -M

EdU Flow Cytometry647-50 Kit+ 500mg EdU

1 Kit

19700

baseclick

BCK488-IV- FC -L

EdU Flow Cytometry488- 50 Kit+ 1000mg EdU

1 Kit

26900

baseclick

BCK555-IV- FC -L

EdU Flow Cytometry555- 50 Kit+ 1000mg EdU

1 Kit

26900

baseclick

BCK594-IV- FC -L

EdU Flow Cytometry594- 50 Kit+ 1000mg EdU

1 Kit

26900

baseclick

BCK647-IV- FC -L

EdU Flow Cytometry647- 50 Kit+ 1000mg EdU

1 Kit

26900

baseclick

BCK488-IV- HTS -S

EdU HTS488- 2×96 Kit + 50mg EdU

1 Kit

5760

baseclick

BCK555-IV- HTS -S

EdU HTS555- 2×96 Kit + 50mg EdU

1 Kit

5760

baseclick

BCK594-IV- HTS -S

EdU HTS594- 2×96 Kit + 50mg EdU

1 Kit

5760

baseclick

BCK488-IV- HTS -M

EdU HTS488- 2×96 Kit + 500mg EdU

1 Kit

16560

baseclick

BCK555-IV- HTS -M

EdU HTS555- 2×96 Kit + 500mg EdU

1 Kit

16560

baseclick

BCK594-IV- HTS -M

EdU HTS594- 2×96 Kit + 500mg EdU

1 Kit

16560

baseclick

BCK488-IV- HTS -L

EdU HTS488- 2×96 Kit + 1000mg EdU

1 Kit

23760

baseclick

BCK555-IV- HTS -L

EdU HTS555- 2×96 Kit + 1000mg EdU

1 Kit

23760

baseclick

BCK594-IV- HTS -L

EdU HTS594- 2×96 Kit + 1000mg EdU

1 Kit

23760

baseclick

BCK-EdUPro-IM488

EdU DetectPro Imaing kit 488 (100 assays)

1 Kit

8000

baseclick

BCK-EdUPro-IM647

EdU DetectPro Imaing kit 647(100 assays)

1 Kit

8400

baseclick

BCK-EdUPro-FC488

EdU DetectPro Flow Cytometry kit 488 (50 assays)

1 Kit

8400

baseclick

BCK-EdUPro-FC647

EdU DetectPro Flow Cytometry kit 647 (50 assays)

1 Kit

8800

baseclick

BCK-EdUPro-HTS488-200

EdU DetectPro HTS kit 488 (2x96well plates or 200 reactions)

1 Kit

6000

baseclick

BCK-EdUPro-HTS488-400

EdU DetectPro HTS kit 488 (4x96well plates or 400 reactions)

1 Kit

10000

baseclick

BCK-EdUPro-HTS555-200

EdU DetectPro HTS kit 555 (2x96well plates or 200 reactions)

1 Kit

6000

baseclick

BCK-EdUPro-HTS555-400

EdU DetectPro HTS kit 555 (4x96well plates or 400 reactions)

1 Kit

10000

baseclick

BCK-OF-PT-488

Arabidopsis-type omer Probe488 (50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-OF-PT-555

Arabidopsis-type omer Probe555 (50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-OF-PT-594

Arabidopsis-type omer Probe594 (50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-OF-PT-Biot

Arabidopsis-type omer ProbeBiotin (50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-OF-HM-488

Human-type omer Probe488 (50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-OF-HM-555

Human-type omer Probe555 (50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-OF-HM-594

Human-type omer Probe594(50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-OF-HM-Biot

Human-type omer ProbeBiotin (50 reactions)

5 µl/ 10*1µg/µl

1900

baseclick

BCK-FISH488

Click FISH-Kit 488

1 Kit

4300

baseclick

BCK-FISH555

Click FISH-Kit 555

1 Kit

4300

baseclick

BCK-FISH594

Click FISH-Kit 594

1 Kit

4300

baseclick

BCK-FISH647

Click FISH-Kit 647

1 Kit

4300

baseclick

BCPep-002

EFT-Peptide Pool MHC Class II

1 vial

9000

baseclick

BCPep-004

CEF-Peptidpool HLA Klasse I

1 vial

3400

baseclick

BCPep-005

CEF- Peptidpool HLA Klasse I PLUS

1 vial

6400

baseclick

BCPep-006

CE Peptidpool HLA-A24

1 vial

4000

baseclick

BCPep-008

Cytomegalovirus Peptidpool

1 vial

4200

baseclick

BCPep-009

Epstein-Barr-Virus Peptidpool

1 vial

4200

baseclick

BCPep-010

Influenza Virus Peptidpool

1 vial

4200

baseclick

BCPep-011

Pre-Dissolving Solution I

1 vial

1100

baseclick

BCK-RNA488-10

RNA Labeling Kit – 488

1 Kit

11600

baseclick

BCK-RNA555-10

RNA Labeling Kit – 555

1 Kit

11600

baseclick

BCK-RNA594-10

RNA Labeling Kit – 594

1 Kit

11600

baseclick

BCK-RNA647-10

RNA Labeling Kit – 647

1 Kit

11600

baseclick

BCK-RNABiotin-10

RNA Labeling Kit – Biotin

1 Kit

11600

baseclick

BCFA-211-5

DAPI

5mg

560

baseclick

BCFA-211-10

DAPI

10mg

900

baseclick

BCFA-211-25

DAPI

25mg

1840

baseclick

BCFA-212-5

Hoechst Staining H33342

5mg

500

baseclick

BCFA-212-10

Hoechst Staining H33342

10mg

720

baseclick

BCFA-212-25

Hoechst Staining H33342

25mg

940

baseclick

BCFA-213-10

DEAE-Dextran

10g

1160

baseclick

BCFA-213-25

DEAE-Dextran

25 g

2100

baseclick

BCFA-214-50

Polyethylenimine

50mL

1700

baseclick

BCFA-214-100

Polyethylenimine

100mL

3000

baseclick

BCFA-111-1

Folate PEG3-Azide

1mg

6000

baseclick

BCFA-111-5

Folate PEG3-Azide

5mg

26000

baseclick

BCFA-028-1

Tocopherol-PEG3-Azide

1mg

6000

baseclick

BCFA-028-5

Tocopherol-PEG3-Azide

5mg

26000

baseclick

BCFA-215-100

GalNAc (N-Acetyl-D-galactosamine)

100mg

1800

baseclick

BCFA-215-250

GalNAc (N-Acetyl-D-galactosamine)

250mg

3360

baseclick

BCFA-216-100

2-Azido-2-deoxy-D-glucose

100mg

2120

baseclick

BCFA-216-250

2-Azido-2-deoxy-D-glucose

250mg

4200

baseclick

BCFA-217-25

6-Azido-6-deoxy-D-glucose

25mg

2640

baseclick

BCFA-217-50

6-Azido-6-deoxy-D-glucose

50mg

5000

baseclick

BCFA-218-25

6-Azido-6-deoxy-D-galactose

25mg

3600

baseclick

BCFA-218-50

6-Azido-6-deoxy-D-galactose

50mg

6860

baseclick

BCFA-226-5

Ac4ManNAz (N-azidoacetylmannosamine-tetraacylated)

5 mg

4100

baseclick

BCFA-226-25

Ac4ManNAz (N-azidoacetylmannosamine-tetraacylated)

25 mg

6800

baseclick

BCFA-227-5

Ac4GalNAz (N-azidoacetylgalactosamine-tetraacylated)

5 mg

4100

baseclick

BCFA-227-25

Ac4GalNAz (N-azidoacetylgalactosamine-tetraacylated)

25 mg

6800

baseclick

BCFA-228-5

Ac4GlcNAz (N-azidoacetylglucosamine-tetraacylated)

5 mg

4100

baseclick

BCFA-228-25

Ac4GlcNAz (N-azidoacetylglucosamine-tetraacylated)

25 mg

6800

baseclick

BCFA-229-5

Ac4ManNAI (N-(4-pentynoyl) -mannosamine tetraacylated)

5 mg

4100

baseclick

BCFA-229-25

Ac4ManNAI (N-(4-pentynoyl) -mannosamine tetraacylated)

25 mg

6800

baseclick

BCFA-230-5

Ac4GalNAI (N-(4-pentynoyl) -galactosamine tetraacylated)

5 mg

4100

baseclick

BCFA-230-25

Ac4GalNAI (N-(4-pentynoyl) -galactosamine tetraacylated)

25 mg

6800

baseclick

BCFA-231-5

Ac4GlcNAI (N-(4-pentynoyl) -glucosamine tetraacylated)

5 mg

4100

baseclick

BCFA-231-25

Ac4GlcNAI (N-(4-pentynoyl) -glucosamine tetraacylated)

25 mg

6800

baseclick

BCSE-001-2

Alkyne agarose resin. 50% slurry

2 mL

4000

baseclick

BCSE-002-2

Azide agarose resin. 50% slurry

2 mL

4000

baseclick

BCSE-003-1

Alkyne Magnetic Beads. 10 mg/mL

1 mL

4200

baseclick

BCSE-004-1

Azide Magnetic Beads. 10 mg/mL

1 mL

4200

baseclick

 

 

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1:强大的进口辐射能力,血清、抗体、耗材、大部分限制进口品等。

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6:我们还是Santa,Advanced Biotechnologies Inc,Athens Research & Technology,bangs,BBInternational,crystalchem,dianova,FD Neurotechnologies,Inc. FormuMax Scientific,Inc, Genebridege, Glycotope Biotechnology GmbH; iduron,Innovative Research of America, Ludger, neuroprobe,omicronbio, Polysciences,prospecbi, QA-BIO,quickzyme,RESEARCH DIETS,INC,sterlitech;sysy,TriLink BioTechnologies,Inc;worthington-biochem,zyagen等几十家国外公司代理。

7:我们还是invitrogen,qiagen,MiraiBioam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等*批发,欢迎合作。

 

 

  

DEAE葡聚糖

上海金畔生物科技有限公司提供DEAE葡聚糖 ,欢迎访问官网了解更多产品信息。

产品编号 D08014
英文名称 DEAE-Dextran hydrochloride
中文名称 DEAE葡聚糖
别    名 diethylaminoethyl dextran hydrochloride; Diethylaminoethyl-dextran HCl; DEAE-Dextran hydrochloride BioReagent, for molecular biology;   DEAE 葡聚糖; DEAE-葡聚糖盐酸盐;
保存条件 Store at 2-8℃
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 CAS:9064-91-9
分子量:500000
MDL:MFCD00081561
来源:bacterial (Leuconostoc mesenteroides)
外观:白色至类白色粉末
纯度:GR
标记范围:~3.2% nitrogen
溶解性:H2O: soluble 100 mg/mL, clear, colorless to faintly yellow
分离范围(MW):肽及球形蛋白质:1000-5000;葡聚糖:500~10000;
产品描述:葡聚糖凝胶G系列产品是一种珠状的凝胶,含有大量的羟基,很容易在水中和电解质溶液中溶胀。亲水基质使其非特异性吸附最小化,并在生物分子分离期间得到较高的回收率。G 型的葡聚糖凝胶有各种不同的交联度,因此它们的溶胀度和分级分离范围也有所不同。葡聚糖凝胶的 溶胀度基本上不因盐和洗涤剂的存在而受影响。葡聚糖凝胶G-50 (Sephadex G-50) 是一种凝胶过滤介质,用于亲和层析、蛋白质色谱和凝胶过滤层析。利用分子筛作用,进行缓冲液交换、脱盐,分离小分子,去除小分子等。

DEAE-葡聚糖溶液(10mg/ml)

上海金畔生物科技有限公司提供DEAE-葡聚糖溶液(10mg/ml) ,欢迎访问官网了解更多产品信息。

产品编号 C7070
英文名称 DEAE-Dextran solution(10mg/ml)
中文名称 DEAE-葡聚糖溶液(10mg/ml)
保存条件 -20℃保存,有效期6个月。
产品介绍 将DNA导入细胞的方法很多,采用DEAE-葡聚糖转染法主要优点是相对简单、快速、重复性较好,该法尤其适用于瞬时转染。DEAE-葡聚糖溶液(10mg/ml)采用特殊溶剂溶解,经过滤除菌,临用前37℃保温并充分混匀。

DEAE纤维素DE-52

DEAE纤维素DE-52

¥866.00

货号:BS207-100g

规格:100g

品牌:biosharp

产品简介:
DEAE 纤维素 DE-52,一种柱色谱使用的弱碱性阴离子交换纤维素。不溶于水、酸、
碱和乙醇。采用平均粒径为 50µm 的颗粒型亲水高分子聚合物,表面又用大分子糖链接
枝,使它有更高的比表面积和更好的生物兼容性,保持更高载量,同时又具有更好的分辨率。由于比表面积大,平衡和洗脱的时间也更短。它经过接枝即使是纯化病毒,质粒等超大分子的物质,载量基本保持不变。
别名:DEAE 纤维素 52;离子交换纤维素;DEAE 纤维素 DE52
CAS:9013-34-7
外观(性状):预溶胀微粒,白色
凝胶类型:纤维素凝胶,离子交换型
结构成分:高度交联纤维素
粒径:50um
配基密度:40umol/ml
吸附载量:180mg HSA/ml
单位:瓶
储存条件:4℃
有效期: 1 年
应用:DEAE-纤维素,它采用平均粒径为 50 um 的颗粒型亲水高分子聚合物,表面又用大分子糖链接枝,使它有更高的比表面积和更好的生物兼容性,它在高流水下保持更高载量,同时又具有更好的分辨率,用于分离生物高分子。
使用方法:
1 色谱柱装填
(1)所需要用到的材料的温度要与色谱操作的温度一样,液体最好做脱气处理。填料可
直接称量需要的量用缓冲液溶涨一小时装柱即可,如果不好溶胀,可适当加热。
(2)在柱子下端加入 20%乙醇,以除去柱子中的空气,关闭柱子出口,在柱内保留少
量的 20%乙醇。20%乙醇容易产生气泡,可以在里面加 1%吐温避免气泡产生。也可以换成纯水装柱子,但是需要把填料中的 20%乙醇也换成纯水,具体的方法取需要体积的填料在抽滤漏斗上进行,也可以小心倾去填料上的 20%乙醇,再换成 5 倍体积的纯水,反复沉淀去上清,5 次左右就可以用于装柱子。
(3)此填料颗粒比较细,所以一定要注意柱子要选择合适的筛网,也可以取点填料加到
筛网上试试,如果没有问题再将填料连续倒入柱子时,要用玻璃棒的紧靠柱子内壁引流,以减少气泡的产生,让填料先自然沉降到填料体积不再变化,而填料和上面的液体很好分层,上层溶液完全澄清,就可以开泵用适当的流速压柱子,填料体积不再变化后,再把转换头紧顶在填料上就可以平衡柱子使用。使用的流速要小于装柱子的流速。
(4)在装柱子前,填料从冰箱中取出至少要室温放置 2-3 个小时,这样避免装柱子时由
于温度变化而使柱子中产生气泡。
2 蛋白的结合
样品的盐浓度和 pH 要尽量和平衡柱子的缓冲液一致,盐浓度过高或者 pH 太低也许挂
不上,所以要根据自己的样品做适当调整。
3 蛋白的洗脱
填料如果采用线性梯度洗脱,柱子的直径和高度比最好是大于 10,数值越大越有利于
分离,而且样品最好别上太多,可以按约 10mg/ml 上样,如果采用阶段洗脱的方法,装短粗柱子就可以,上样量也没有限制。阶段洗脱容易放大,重复性好,如果洗脱条件好完全可以得到和线性梯度一样或者更好。
4 再生清洗
(1)每次用完最好用 0.1M NaOH 含 2M NaCl 洗 5 个柱床体积,再用水洗 5 个柱床体
积,然后用 20%乙醇保存,使用 3-5 次后在水洗之后再用 70%的乙醇或 30%异丙醇(含1%吐温)洗 5 个柱床体积,最后 20%的乙醇流洗 5 个柱床体积。
(2)有机溶剂和水混合很容易产生气泡,为了避免这样情况,可以将配好的有机溶剂在
室温放置过夜,再使用,这样可以避免气泡进柱子而导致柱子不能正常使用。
注意:
1. 将本品置于通风、干燥的地方保存,不能冷冻。
2. 在装柱、使用和保存柱子的时候,要避免柱子流干,气泡进入。
3. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。
4. 为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
货号 BS207-100g
规格 100g
品牌 biosharp
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  • DEAE纤维素DE-52