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Exonuclease III (E. coli) |NEB酶试剂 New England Biolabs

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Exonuclease III (E. coli)

Exonuclease III (E. coli) |

  • Double-stranded DNA specific exonuclease
  • Initiates at the 3′ termini of linear double-stranded DNA with 5′ overhangs or blunt ends and 3′ overhangs containing less than four bases
  • Initiates at nicked sites in double-stranded DNA
  • Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 3′ to 5′ direction

Exonuclease III is ideal for:

  • Site-directed mutagenesis
  • Preparation of single-stranded DNA for dideoxy sequencing
  • Preparation of nested deletions in double-stranded DNA