标签归档：factor

人纤维蛋白原Human Fibrinogen 2（Factor I）说明书

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  Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research 代理,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。

Human Fibrinogen （Factor I）

Human Fibrinogen

FIB 2 Plasminogen and von Willebrand Factor Depleted

 

Conversion of soluble fibrinogen to the insoluble fibrin is the final step in the blood coagulation cascade. ERL offers three types of purified fibrinogen prepared by a combination of salt precipitation, affinity chromatography and immunoaffinity chromatography methods to suit the individual researcher’s needs. All three forms are >95% clottable （by functional assays） and purity is >95% by SDS-PAGE.

 

  • FIB 1 Plasminogen Depleted
  • FIB 2 Plasminogen and von Willebrand Factor Depleted
  • FIB 3 Plasminogen, von Willebrand Factor and Fibronectin Depleted

 

Buffer composition = 20 mM Sodium Citrate-HCl/pH 7.4
*Extinction Coefficient （1%） = 15.1
Molecular Weight = 330,000 daltons

 

 

人纤维蛋白原Human Fibrinogen 1（Factor I）说明书

发表于由

 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research 代理,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。

Human Fibrinogen （Factor I）

Human Fibrinogen   

FIB 1 Plasminogen Depleted

 

Conversion of soluble fibrinogen to the insoluble fibrin is the final step in the blood coagulation cascade. ERL offers three types of purified fibrinogen prepared by a combination of salt precipitation, affinity chromatography and immunoaffinity chromatography methods to suit the individual researcher’s needs. All three forms are >95% clottable （by functional assays） and purity is >95% by SDS-PAGE.

 

  • FIB 1 Plasminogen Depleted
  • FIB 2 Plasminogen and von Willebrand Factor Depleted
  • FIB 3 Plasminogen, von Willebrand Factor and Fibronectin Depleted

 

Buffer composition = 20 mM Sodium Citrate-HCl/pH 7.4
*Extinction Coefficient （1%） = 15.1
Molecular Weight = 330,000 daltons

人凝血因子XIIIa（Human Factor XIIIa）HFXIIIa 1314说明书

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 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research 代理,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human Factor XIIIa

 

HFXIIIa 1314        Human Factor XIIIa

Human Factor XIII is cleaved with human alpha thrombin.  The thrombin is subsequently removed via chromatography.  The above protein was purified from Human plasma that was tested and found negative by FDA accepted methods fro Anti-HIV1/2, Anti-HTLV I & II, HBsAg, Anti-HCV, Syphilis, ABC ab, HIV-1 p24 Ag or HIV-1 RNA, HCV RNA and HBV RNA.  Donors are screened for CJD （Creutzfeldt-Jakob Disease）. 

Buffer composition =  50 mM Tris/ 100 mM NaCl/ 1 mM EDTA/ 10 u/mL aprotinin/ 50 uM DTT/50% glycerol/ pH 7.5
*Extinction Coefficient （1%）=13.8
Molecular Weight = 312,000 daltons

 

人类α凝血因子（Human Factor αXIIa）HFXIIa 1212a说明书

发表于由

 Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Enzyme Research Laboratories为生物技术公司,制药行业、医院和大学的研究实验室提供用于的纯化抗凝因子。除了其制造能力，Enzyme Research Laboratories也创新研发领域中的血液凝固技术。

Human Factor αXIIa

 

HFXIIa 1212a             Human Factor αXIIa 
                              （Activated Hageman Factor）

Human Factor α-XIIa is a serine protease responsible for the activation of Factor XI to XIa in the contact activation system. Human Factor XII and prekallikrein are thought to be involved in a reciprocal activation mechanism in which Factor XIIa activates prekallikrein to kallikrein, which in turn converts Factor XII to XIIa. Factor XIIa activates Factor XI to XIa thereby triggering the Contact Factor cascade. ERL offers Factor α-XIIa which is activated by the autoactivation process with Dextran Sulfate and re-purified to remove the activator. The protein purity is determined by SDS-PAGE and activity is determined via clotting assay.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
*Extinction Coefficient （1%） = 14.1
Molecular Weight = 80,000 daltons


 

人凝血因子XIa（Human Factor XIa）HFXIa 1111a 说明书

发表于由

 Enzyme Research ，Enzyme Research 介绍,Enzyme Research 代理,Enzyme Research 代理,Enzyme Research *代理,Enzyme Research 中国代理

Enzyme Research 是一家拥有超过25年的酶研究的实验室，生产和开发多种用于基本凝血研究的多种酶和辅因子。在过去的十年Enzyme Research Laboratories为科学家参与止血和血栓形成的研究提供了宝贵支持。Enzyme Research通过不断追求产品的zui高品质，赢得了世界各地凝血研究实验室的信任。

Human Factor XIa

 

HFXIa 1111a             Human Factor XIa

Prepared from Human Factor XI using Human Factor Xlla. This Xlla was removed using a corn trypsin inhibitor column. Complete activation is observed by SDS-PAGE. Factor XI, through the contact factor pathway cascade, is activated to Factor XIa via Factor Xlla and High Molecular Weight Kininogen. During activation by Factor Xlla and HK, FXI undergoes proteolytic cleavage in which the Mr=80,000 chain reportedly is cleaved to a heavy and light chain with Mr of about 48,000 and 33,000. This Factor XIa is responsible for the activation of Factor IX to Factor IXa. Unlike other examples of activation of Vitamin K-dependent blood-clotting proteins, Factor XIa proteolysis of Factor IX does not require membrane surfaces.

Buffer composition = 4 mM Sodium Acetate-HCl/0.15 M NaCl/pH 5.3
*Extinction Coefficient （1%） = 13.1
Molecular Weight = 160,000 daltons