Uracil-N-Glycosylase(UNG),货号-规格:CW0951S-200U
市场价: | ¥198.0 | |||||||||||||||||||
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品牌: | 康为世纪 | |||||||||||||||||||
规格: | 200U |
产品详情
说明书下载
参考文献
Uracil-N-Glycosylase(UNG),货号-规格:CW0951S-200U 尿嘧啶-N-糖基化酶(UNG酶)也称为尿嘧啶DNA糖基化酶(UDG酶),通过大肠杆菌表达纯化的重组酶,该蛋白分子量为25 kD,可催化含尿嘧啶的单链和双链DNA释放游离尿嘧啶,并且对RNA无活性,主要应用于防止PCR扩增产物的污染。该酶作用机理为:在PCR反应中以dUTP代替dTTP,扩增产物片段中的T全部被U取代,形成了含dU碱基的PCR扩增产物。UNG酶能选择性断裂单链和双链DNA中U碱基的糖苷键,降解反应体系中含U的DNA,有效消除PCR产物的残留污染,大大降低扩增产物污染导致的假阳性,从而保证扩增的特异性和准确性。 Uracil-N-Glycosylase(UNG) 立即下载 |
Uracil-N-Glycosylase(UNG),货号-规格:CW0951M-1000U
Uracil-N-Glycosylase(UNG),货号-规格:CW0951M-1000U
市场价: | ¥798.0 | |||||||||||||||||||
价格: |
|
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品牌: | 康为世纪 | |||||||||||||||||||
规格: | 1000U |
产品详情
说明书下载
参考文献
Uracil-N-Glycosylase(UNG),货号-规格:CW0951M-1000U 尿嘧啶-N-糖基化酶(UNG酶)也称为尿嘧啶DNA糖基化酶(UDG酶),通过大肠杆菌表达纯化的重组酶,该蛋白分子量为25 kD,可催化含尿嘧啶的单链和双链DNA释放游离尿嘧啶,并且对RNA无活性,主要应用于防止PCR扩增产物的污染。该酶作用机理为:在PCR反应中以dUTP代替dTTP,扩增产物片段中的T全部被U取代,形成了含dU碱基的PCR扩增产物。UNG酶能选择性断裂单链和双链DNA中U碱基的糖苷键,降解反应体系中含U的DNA,有效消除PCR产物的残留污染,大大降低扩增产物污染导致的假阳性,从而保证扩增的特异性和准确性。 Uracil-N-Glycosylase(UNG) 立即下载 |
Afu Uracil-DNA Glycosylase (UDG) |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Afu Uracil-DNA Glycosylase (UDG)
- Archaeoglobus fulgidus Uracil DNA Glyocsylase
- Thermostable monofunctional DNA glycosylase that catalyzes the hydrolysis of the N-glycosidic bond from deoxyuridine to release uracil
- Active on ss and dsDNA
- Heat activation not possible
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What are endonucleases and their applications?
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Endonuclease III (Nth) |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Endonuclease III (Nth)
- Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
- The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and 5, 6-dihydroxythymine, generating an AP site. The AP lyase activity cleaves an AP site via β-elimination, creating a 1-nucleotide gap with 3′-α, β-unsaturated aldehyde and 5′-phosphate termini
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What are endonucleases and their applications?
Endonuclease VIII |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Endonuclease VIII
- Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
- The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via β and δ-elimination, creating a 1 nucleotide DNA gap with 5′ and 3′ phosphate termini