Hampton Research Mounted CryoLoop HR4-313

Hampton Research Mounted CryoLoop HR4-313

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商,其产品应用广泛且深受广大用户的认可。

Mounted CryoLoop™ – 20 micron

HR4-313,Mounted CryoLoop – 20 micron,0.025 – 0.05 mm – 25 pack

HR4-625,Mounted CryoLoop – 20 micron,0.05 – 0.1 mm – 25 pack

HR4-955,Mounted CryoLoop – 20 micron,0.1 – 0.2 mm – 25 pack

HR4-957,Mounted CryoLoop – 20 micron,0.2 – 0.3 mm – 25 pack

HR4-959,Mounted CryoLoop – 20 micron,0.3 – 0.4 mm – 25 pack

HR4-961,Mounted CryoLoop – 20 micron,0.4 – 0.5 mm – 25 pack

HR4-963,Mounted CryoLoop – 20 micron,0.5 – 0.7 mm – 25 pack

HR4-965,Mounted CryoLoop – 20 micron,0.7 – 1.0 mm – 25 pack

APPLICATIONS

Cryocrystallography


FEATURES

Synthetic CryoLoop on stainless steel MicroTube

MicroTube specially engineered with EasySnap notches

Complete range of loop diameters from 0.025 – 1.0 mm

Compatible with all CrystalCap Systems


DESCRIPTION

Note: If you are using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT, or CrystalCap SPINE HT, save time and money by using the ready to mount 18 mm Mounted CryoLoop.

Mounted CryoLoops with 20 micron diameter nylon. These nylon loops are bonded to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and secure to the CrystalCap using epoxy or similar adhesive. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic..

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicroTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.


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Hampton Research 18 mm Siliconized circle cover slides

Hampton Research 18 mm Siliconized circle cover slides

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商,其产品应用广泛且深受广大用户的认可。

18 mm Siliconized Glass Cover Slides

HR3-239,18 mm x 0.22 mm Siliconized circle cover slides

HR3-241,18 mm x 0.22 mm Siliconized circle cover slides

HR3-515,18mmx0.96mm Thick Siliconized circle cover slides

HR3-517,18mmx0.96mm Thick Siliconized circle cover slides

Hampton Research 18 mm Siliconized circle cover slides


APPLICATIONS

Siliconized glass cover slides for hanging & sitting drop crystallization

Hanging drop crystallization

Sitting drop crystallization

Sandwich drop crystallization


FEATURES

High purity of glass

Siliconized, hydrophobic glass surface

0.22 mm & 0.96 mm glass thickness

18 mm glass diameter

Circle

0.22 mm glass thickness is UV Compatible (>85% transmission)


DESCRIPTION

Siliconized glass cover slides allow a droplet to be suspended in a position which provides near optimal conditions for vapor diffusion with the surrounding reservoir solution.

These siliconized glass cover slides provide a consistent, high quality finish for crystallization experiments. The hydrophobic surface produces a drop which “stands well” and does not flatten on the glass. The siliconized surface prevents the adhesion of crystals and precipitate onto the glass surface. Use Dow Corning Vacuum Grease (HR3-510) or Dow Corning 7 Release Compound (HR3-508) to seal cover slide to plate. Available in 12, 18 or 22 mm diameter circles and 22 mm diameter squares. Available in 0.22 mm or 0.96 mm glass thickness. The 0.96 mm thick slides are virtually unbreakable during crystallization handling.

Critical surface tension Untreated glass 78 dynes/cm Siliconized glass 31 dynes/cm Surface resistivity Untreated glass 1×1012 ohms Siliconized glass 1.2 x 1013 ohms Coefficient of friction static, glass slide on glass slide Untreated 0.9-1.0 Siliconized 0.2-0.3 Protein adsorption (100 hours) Untreated glass 0.13 mg/mm2 Siliconized glass 0.01-0.02 mg/mm2


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Hampton Research MASTERBLOCK® 96 Deep Well (Greiner) HR3-105

Hampton Research MASTERBLOCK® 96 Deep Well (Greiner) HR3-105

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商,其产品应用广泛且深受广大用户的认可。

MASTERBLOCK® 96 Deep Well (Greiner)

HR3-105 ,MASTERBLOCK® 96 Deep Well polypropylene plate

HR3-103,Cap Mat for MASTERBLOCK®

Hampton Research MASTERBLOCK® 96 Deep Well (Greiner) HR3-105

APPLICATIONS

96 Deep Well block for crystallization screen reagents


FEATURES

SBS format, 96 well

Sterile, individually sealed plates

Polypropylene

Seal with AlumaSeal II, Crystal Clear Sealing Film, or Cap Mats


DESCRIPTION

The Greiner 780261 MASTERBLOCK® is a 96 Deep Well polypropylene block (plate). Each well has a fill volume of 1.2 ml. The MASTERBLOCK® is useful for holding crystallization screen reagents when using liquid handling automation or multi channel pipets. Each plate is sterile and packaged individually. Round bottom well for complete reagent recovery. ClearChoice™ virgin polypropylene resin (autoclavable to 121°C). SBS recommended dimensions. Alphanumerically coded wells with chimney-style, round well top.

The Microplate Lid is a temporary cover for the crystallization screens in 96 deep well format. The single position, high profile cover with individual condensation rings over each reagent well can help minimize reagent evaporation from 96 deep well reagent plates as well as protect the reagents from particulate and contamination. The individually wrapped, sterile Microplate Lid is made from optically clear polystyrene. Greiner 656171.

The Cap Mat is made of ethyl vinyl acetate (EVA). Resistant to DMSO and can be used in a temperature range between -20 and +60 degrees Celsius. Suitable for sealing Greiner 780261 1 milliliter Deep Well Plate (HR3-105). These cap mats are not piercable. Supplied sterile. Greiner part number 381061.

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Hampton Research HR2-144 Index,HR2-134 Index HT

Hampton Research HR2-144 Index,HR2-134 Index HT

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商,其产品应用广泛且深受广大用户的认可。


Index • Index HT

HR2-144,Index,10 ml, tube format

HR2-134,Index HT,1 ml, Deep Well block format


APPLICATIONS

Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules


FEATURES

Developed at Hampton Research

A data-driven biased sparse matrix and grid screen

Screens classic, contemporary, & modern crystallization reagents

Samples pH 3 to 9

Compatible with microbatch, vapor diffusion, & liquid diffusion methods

Specially formulated reagent zones:

Traditional salts versus pH

Neutralized organic acids

High [salt] with low [polymer]

High [polymer] with low [salt]

Low ionic strength versus pH

PEG & Salt versus pH

PEG & Salt

Tube or Deep Well block format


DESCRIPTION

Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format. 

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.

Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.


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Hampton Research HR2-126 PEG/Ion Screen

Hampton Research HR2-126 PEG/Ion Screen

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商,其产品应用广泛且深受广大用户的认可。

PEG/Ion • PEG/Ion 2 • PEG/Ion HT


HR2-126 ,PEG/Ion Screen 10 ml, tube format

HR2-098, PEG/Ion 2 Screen 10 ml, tube format

HR2-139, PEG/Ion HT 1 ml, Deep Well block format


APPLICATIONS

Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules


FEATURES

Developed at Hampton Research

PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2

PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350

PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block


DESCRIPTION

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules.

The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.

PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.

金畔生物代理美国Hampton Research,负责其在中国的销售与推广,更多产品信息,欢迎与我们联系