标签归档：his

pEF1/myc-His B载体说明书

发表于由

型号 载体名称 出品公司 载体用途 价格
VPI0027 pEF1/myc-His B Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pEF1/myc-His A载体说明书

发表于由

型号 载体名称 出品公司 载体用途 价格
VPI0026 pEF1/myc-His A Invitrogen 哺乳动物表达载体 800

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Neo，G418
Promoter：EF-1α
Vector Type：pEF
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：c-Myc Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 

氨苄青霉素（Ampicillin）

载体描述: 

pEF1/myc-His A, B, and C are 6.2 kb vectors derived from pcDNA 3.1/myc-His and designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells. The vectors contain the following elements:

  1. Human elongation factor 1?-subunit （hEF-1α） promoter for high-level expression across a broad range of species and cell types （Goldman et al., 1996; Mizushima and Nagata, 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal tag encoding the myc （c-myc） epitope and a polyhistidine metal-binding peptide.
  3. Neomycin resistance gene for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g., COS7）.

The control plasmid, pEF1/myc-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 

pUB6/V5 His C说明书

发表于由

 

 pUB6/V5 His C

型号 载体名称 出品公司 载体用途
VPI0024 pUB6/V5 His C Invitrogen 哺乳动物表达载体

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
  3. Blasticidin resistance gene （bsd） for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 
 

pUB6/V5 His B说明书

发表于由

 pUB6/V5 His B

型号 载体名称 出品公司 载体用途
VPI0023 pUB6/V5 His B Invitrogen 哺乳动物表达载体

 

产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
  3. Blasticidin resistance gene （bsd） for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: 
 

 

 

 

pUB6/V5 His A说明书

发表于由

 pUB6/V5 His A

型号 载体名称 出品公司 载体用途
VPI0022 pUB6/V5 His A Invitrogen 哺乳动物表达载体
产品参数: 

Key Function：Constitutive Expression
Mammalian Selection：Blasticidin
Promoter：UbC
Vector Type：pUB6
Cloning Method：Restriction Enzyme/MCS 
Protein Tag or Fusion：V5 Epitope Tag，His Tag （6x His） 
Constitutive or Inducible System：Constitutive

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

pUB6/V5-His A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. Features of the vectors allow purification and detection of expressed proteins. High-level stable and transient expression can be carried out in most mammalian cells.

The vectors contain the following elements:

  1. Human ubiquitin C promoter （hUbC） for high-level expression across a broad range of species and cell types （Schorpp et al., 1996; Wulff et al., 1990）.
  2. Three reading frames to facilitate in-frame cloning with a C-terminal peptide encoding the V5 epitope and a polyhistidine （6×His） metal-binding tag.
  3. Blasticidin resistance gene （bsd） for selection of stable cell lines.
  4. Episomal replication in cell lines that are latently infected with SV40 or that express the SV40 large T antigen （e.g. COS-1, COS-7）.

The control plasmid, pUB6/V5-His/lacZ, is included for use as a positive control for transfection, expression, and detection in the cell line of choice.

质粒图谱: