上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Hot Start Taq 2X Master Mix
The industry standard with the hot start peformance
Ideal for routine PCR applications
Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
2X master mix format for easy reaction setup – just add template DNA and primers
产品信息
Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It is recommended for amplification up to 5 kb.
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Hot Start Taq DNA Polymerase possesses a 5´→ 3´ polymerase activity1,2,3 and a 5´ flap endonuclease activity.4,5
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
产品类别:
Taq DNA Polymerase Products,
Master Mixes Products
应用:
Multiplex PCR,
Specialty PCR,
Routine PCR,
PCR
产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB #
名称
组分货号
储存温度
数量
浓度
M0496S
-20
Hot Start Taq 2X Master Mix
M0496SVIAL
-20
2 x 1.25 ml
2 X
M0496L
-20
Hot Start Taq 2X Master Mix
M0496SVIAL
-20
10 x 1.25 ml
2 X
特性和用法
1X 预混液组分
10 mM Tris-HCl 50 mM KCl 1.5 mM MgCl2 0.2 mM dNTPs 5% Glycerol 25 units/ml Hot Start Taq DNA polymerase pH 8.3 @ 25°C
热失活
否
单位活性检测条件
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
优势和特性
应用特性
High-specificity PCR
Routine PCR
High-throughput PCR
Microarray Analysis
Colony PCR
相关产品
相关产品
Deoxynucleotide (dNTP) Solution Set
Deoxynucleotide (dNTP) Solution Mix
Hot Start Taq DNA Polymerase
Magnesium Chloride (MgCl2) Solution
Standard Taq Reaction Buffer Pack
注意事项
Hot Start Taq 2X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C.
Hot Start Taq 2X Master Mix is also stable for three months at 4°C, so for frequent use, an aliquot may be kept at 4°C.
参考文献
Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, PubMedID: 1550-1557
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Hot Start Taq DNA Polymerase
The industry standard with hot start performance
Ideal for routine PCR applications and molecular diagnostics
High specificity and sensitivity
Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
精选视频
Choose the Right DNA Polymerase for PCR
观看其他视频
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders. Learn More
产品信息
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity (1)(2)(3)and a 5´ flap endonuclease activity(4)(5).
It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
产品类别:
Taq DNA Polymerase Products
应用:
Colony PCR,
Multiplex PCR,
Specialty PCR,
Routine PCR,
PCR
产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB #
名称
组分货号
储存温度
数量
浓度
M0495S
-20
Hot Start Taq DNA Polymerase
M0495SVIAL
-20
1 x 0.04 ml
5,000 units/ml
Standard Taq Reaction Buffer Pack
B9014SVIAL
-20
1 x 1.5 ml
10 X
M0495L
-20
Hot Start Taq DNA Polymerase
M0495LVIAL
-20
1 x 0.2 ml
5,000 units/ml
Standard Taq Reaction Buffer Pack
B9014SVIAL
-20
3 x 1.5 ml
10 X
特性和用法
单位定义
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
反应条件
1X Standard Taq Reaction Buffer Pack
1X Standard Taq Reaction Buffer Pack 10 mM Tris-HCl 50 mM KCl 1.5 mM MgCl2 (pH 8.3 @ 25°C)
贮存溶液
10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 1X stabilizers pH 7.4 @ 25°C
热失活
否
单位活性检测条件
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
优势和特性
应用特性
High-specificity PCR
Routine PCR
Microarray Analysis
Colony PCR
相关产品
相关产品
Deoxynucleotide (dNTP) Solution Set
Deoxynucleotide (dNTP) Solution Mix
Hot Start Taq 2X Master Mix
Magnesium Chloride (MgCl2) Solution
Standard Taq Reaction Buffer Pack
参考文献
Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
Hot Start Taq 2X Master Mix
The industry standard with the hot start peformance
Ideal for routine PCR applications
Aptamer-based hot start technology avoids nonspecific amplification and enables room temperature reaction setup
2X master mix format for easy reaction setup – just add template DNA and primers
产品信息
Hot Start Taq 2X Master Mix is an optimized ready-to-use solution containing Hot Start Taq DNA Polymerase, dNTPs, MgCl2, KCl and stabilizers. It is ideally suited to routine PCR applications from templates including pure DNA solutions, bacterial colonies, and cDNA products. It is recommended for amplification up to 5 kb.
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Hot Start Taq DNA Polymerase possesses a 5´→ 3´ polymerase activity1,2,3 and a 5´ flap endonuclease activity.4,5
产品来源
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
产品类别:
Taq DNA Polymerase Products,
Master Mixes Products
应用:
Multiplex PCR,
Specialty PCR,
Routine PCR,
PCR
产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB #
名称
组分货号
储存温度
数量
浓度
M0496S
-20
Hot Start Taq 2X Master Mix
M0496SVIAL
-20
2 x 1.25 ml
2 X
M0496L
-20
Hot Start Taq 2X Master Mix
M0496SVIAL
-20
10 x 1.25 ml
2 X
特性和用法
1X 预混液组分
10 mM Tris-HCl 50 mM KCl 1.5 mM MgCl2 0.2 mM dNTPs 5% Glycerol 25 units/ml Hot Start Taq DNA polymerase pH 8.3 @ 25°C
热失活
否
单位活性检测条件
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
优势和特性
应用特性
High-specificity PCR
Routine PCR
High-throughput PCR
Microarray Analysis
Colony PCR
相关产品
相关产品
Deoxynucleotide (dNTP) Solution Set
Deoxynucleotide (dNTP) Solution Mix
Hot Start Taq DNA Polymerase
Magnesium Chloride (MgCl2) Solution
Standard Taq Reaction Buffer Pack
注意事项
Hot Start Taq 2X Master Mix is stable for fifteen freeze-thaw cycles when stored at -20°C.
Hot Start Taq 2X Master Mix is also stable for three months at 4°C, so for frequent use, an aliquot may be kept at 4°C.
参考文献
Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, PubMedID: 1550-1557