上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
The industry standard with hot start performance
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity (1)(2)(3)and a 5´ flap endonuclease activity(4)(5).
It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
PCR
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||||||||||||||
|
||||||||||||||||||||||||
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
1X Standard Taq Reaction Buffer Pack
1X Standard Taq Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
(pH 8.3 @ 25°C)
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
1X stabilizers
pH 7.4 @ 25°C
否
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
The industry standard with the hot start peformance
产品信息
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions allowing reactions to be set up at room temperature. The aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Hot Start Taq DNA Polymerase possesses a 5´→ 3´ polymerase activity1,2,3 and a 5´ flap endonuclease activity.4,5
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1
PCR
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||||||||
|
||||||||||||||||||
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
0.2 mM dNTPs
5% Glycerol
25 units/ml Hot Start Taq DNA polymerase
pH 8.3 @ 25°C
否
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
The industry standard with hot start performance
Interested in glycerol-free or custom formulations? – contact our Custom Solutions Group
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity (1)(2)(3)and a 5´ flap endonuclease activity(4)(5).
It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.
An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1.
PCR
本产品提供以下试剂或组分：
| NEB # | 名称 | 组分货号 | 储存温度 | 数量 | 浓度 | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
||||||||||||||||||||||||
|
||||||||||||||||||||||||
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.
1X Standard Taq Reaction Buffer Pack
1X Standard Taq Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
1.5 mM MgCl2
(pH 8.3 @ 25°C)
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
1X stabilizers
pH 7.4 @ 25°C
否
1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13 DNA.
操作说明、说明书 & 用法
工具 & 资源
FAQs & 问题解决指南
引用 & 技术文献
Powered by Bioz See more details on Bioz质控、安全 & 法规
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
biotechrabbit 2X Multiplex Hot-Start PCR Master Mix说明书
Taq
diatheva NEW Hot Rescue DNA聚合酶说明书
新的Hot Rescue DNA聚合酶是一种热启动 Taq,具有灵敏度和非凡的效率,并且由于有效的3-5'外切核酸酶活性而具有更高的精度。酶以5 U/uL的浓度提供，含有2 mM氯化镁和10 mM dNTP混合物的10X PCR缓冲液。
货号: MBR0001-D
diatheva MBR0001-D描述
新的Hot Rescue DNA聚合酶可扩增3'末端带有一个A的PCR产物，因此它们可以直接克隆到T载体中，克隆效率>80%。在末端钝化和磷酸化后，也可以将PCR产物克隆到平末端载体中。新的Hot Rescue DNA聚合酶具有高效的校对活性，比任何其他Taq具有更高的保真度。
附加信息
尺寸
250 U
应用
生命科学
产品类别
研究用试剂
试剂类型
聚合酶链反应
类别
分子生物学
技术信息
实时荧光定量PCR
