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Hampton Research Mounted CryoLoop HR4-313

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Hampton Research Mounted CryoLoop HR4-313

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商，其产品应用广泛且深受广大用户的认可。

Mounted CryoLoop™ – 20 micron

HR4-313，Mounted CryoLoop – 20 micron，0.025 – 0.05 mm – 25 pack

HR4-625，Mounted CryoLoop – 20 micron，0.05 – 0.1 mm – 25 pack

HR4-955，Mounted CryoLoop – 20 micron，0.1 – 0.2 mm – 25 pack

HR4-957，Mounted CryoLoop – 20 micron，0.2 – 0.3 mm – 25 pack

HR4-959，Mounted CryoLoop – 20 micron，0.3 – 0.4 mm – 25 pack

HR4-961，Mounted CryoLoop – 20 micron，0.4 – 0.5 mm – 25 pack

HR4-963，Mounted CryoLoop – 20 micron，0.5 – 0.7 mm – 25 pack

HR4-965，Mounted CryoLoop – 20 micron，0.7 – 1.0 mm – 25 pack

APPLICATIONS

Cryocrystallography


FEATURES

Synthetic CryoLoop on stainless steel MicroTube

MicroTube specially engineered with EasySnap notches

Complete range of loop diameters from 0.025 – 1.0 mm

Compatible with all CrystalCap Systems


DESCRIPTION

Note: If you are using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT, or CrystalCap SPINE HT, save time and money by using the ready to mount 18 mm Mounted CryoLoop.

Mounted CryoLoops with 20 micron diameter nylon. These nylon loops are bonded to hollow, stainless steel MicroTubes™ that are used to mount, freeze, and secure the crystal during cryocrystallographic procedures and X-ray data collection. The MicroTube is 24 mm in length and is specially engineered with EasySnap™ notches at the 10, 12, 14, 18, and 21 mm measures. To obtain the desired length of MicroTube, simply snap the MicroTube at the desired length and secure to the CrystalCap using epoxy or similar adhesive. These nylon loops show minimal diffraction, are thin for fast freezing, strong, and aerodynamic..

Most data collection geometries prefer or require a 22 mm length between the base of the cap and the beam. For a 22 mm overall length using the CrystalCap, CrystalCap ALS, CrystalCap ALS HT and CrystalCap SPINE HT, snap the MicroTube at the second notch from the bottom, creating an 18 mm MicroTube lenghth and secure to the CrystalCap using epoxy or similar adhesive. For a 22 mm over length using the CrystalCap Copper, CrystalCap Copper HT, CrystalCap ALS Copper HT snap the MicroTube at the fifth notch from the bottom (notch closest to the CryoLoop) and secure to the CrystalCap using epoxy or similar adhesive.


金畔生物代理美国Hampton Research，负责其在中国的销售与推广。更多产品信息，欢迎直接与我们联系~

Hampton Research HR2-144 Index，HR2-134 Index HT

发表于由
Hampton Research HR2-144 Index，HR2-134 Index HT

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商，其产品应用广泛且深受广大用户的认可。


Index • Index HT

HR2-144，Index，10 ml, tube format

HR2-134，Index HT，1 ml, Deep Well block format


APPLICATIONS

Primary, diverse reagent system crystallization screen for proteins, complexes, peptides, nucleic acids, & water soluble small molecules


FEATURES

Developed at Hampton Research

A data-driven biased sparse matrix and grid screen

Screens classic, contemporary, & modern crystallization reagents

Samples pH 3 to 9

Compatible with microbatch, vapor diffusion, & liquid diffusion methods

Specially formulated reagent zones:

Traditional salts versus pH

Neutralized organic acids

High [salt] with low [polymer]

High [polymer] with low [salt]

Low ionic strength versus pH

PEG & Salt versus pH

PEG & Salt

Tube or Deep Well block format


DESCRIPTION

Index is designed as a 96 reagent crystallization screen that combines the strategies of the grid, sparse matrix, and incomplete factorial screening with traditional, contemporary, and new crystallization reagent systems into a highly effective and efficient format. 

Index, as the name implies, efficiently samples a series of specially formulated reagent zones to identify which reagent class or classes and pH are effective in producing crystals or limiting sample solubility. Results from Index can be used to design optimization experiments and to identify follow on screens by reagent class. For example, positive results with salt based reagents in Index may be followed up with further screening using SaltRx or Grid Screen Salt HT. Success with polymer based reagents in Index may be followed up with further screening using PEGRx or PEG/Ion.

Index utilizes a broad, yet refined portfolio of crystallization reagent systems. These include the following: (1) traditional salts such as Ammonium sulfate and Sodium chloride versus pH; (2) neutralized organic acids such as Sodium malonate and Tacsimate; (3) High salt concentration mixed with low polymer concentration as well as high polymer concentration mixed with low salt concentration and; (4) Low ionic strength using polymers such as PEG, MPD, Pentaerythritols versus pH. These reagent systems are formulated across a sparse matrix and incomplete factorial of concentration ranges, sampling a pH range of 3 to 9.


金畔生物代理美国Hampton Research，负责其在中国的销售与推广，更多产品信息，欢迎与我们联系

Hampton Research HR2-126 PEG/Ion Screen

发表于由
Hampton Research HR2-126 PEG/Ion Screen

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商，其产品应用广泛且深受广大用户的认可。

PEG/Ion • PEG/Ion 2 • PEG/Ion HT


HR2-126 ，PEG/Ion Screen 10 ml, tube format

HR2-098， PEG/Ion 2 Screen 10 ml, tube format

HR2-139， PEG/Ion HT 1 ml, Deep Well block format


APPLICATIONS

Primary or secondary, polymer, salt and pH matrix crystallization screen for biological macromolecules


FEATURES

Developed at Hampton Research

PEG/Ion is a sparse matrix profile of anions and cations in the presence of monodisperse Polyethylene glycol 3,350 over pH 4.5 – 9.2

PEG/Ion 2 screens a complete profile of titrated organic acids at varying pH levels (3.7 – 8.8) in the presence of monodisperse PEG 3,350

PEG/Ion HT combines PEG/Ion and PEG/Ion 2 in a single 96 Deep Well block


DESCRIPTION

PEG/Ion, developed by Hampton Research, is a crystallization screen designed to evaluate monodisperse, high purity Polyethylene glycol 3,350 and 48 unique salts representing a very complete range of anions and cations frequently used in the crystallization of biological macromolecules.

The primary screening variables are PEG, ion type, ionic strength, and pH. More than 60% of the published crystallizations utilized PEG as a primary crystallization reagent and in approximately 50% of those reports, the PEG was combined with an ion as a secondary crystallization reagent. PEG/Ion reagents are formulated without a buffer and are not pH titrated.

PEG/Ion 2 is an extension to the fundamental crystallization strategy in PEG/Ion. PEG/Ion 2 reagents cover the monodisperse, high purity Polyethylene glycol 3,350 and an array of neutralized and pH adjusted organic acids, multivalent ions, a novel Citrate BIS-TRIS propane buffer system and pH (4 – 8.8). The formulation of PEG/Ion 2 was developed at Hampton Research. Each of the 48 reagents in PEG/Ion 2 contains PEG 3,350 as the polymer (precipitant). The concentration of PEG is varied from 12% w/v to 20% w/v depending upon the type and concentration of buffer/salt paired with the polymer. Thirteen of the forty-eight PEG/Ion 2 reagents contain a separate buffer component. The remaining PEG/Ion 2 reagents are buffered by the titrated organic acid salt. Six of these thirteen conditions feature a novel Citric acid BIS-TRIS propane (CBTP) buffer. The CBTP buffer uses Citric acid and BIS-TRIS propane as the acid base pair to create a two component buffer system effective across pH 2.5 to 9.5. The ratio of Citric acid to BIS-TRIS propane determines the solution pH. Thirty-five of the forty-eight PEG/Ion 2 reagents contain a neutralized or pH adjusted organic acid in the presence of the polymer. Neutralized organic acids are highly effective crystallization salts.1 Four PEG/Ion 2 reagents feature polyvalent cations. Two of these reagents contain cation mixes, saving sample by screening six different cations with only two reagents. Tryptone, a casein digest combinatorial library of peptides, is included in PEG/Ion 2. PEG/Ion 2 reagents 1-30, 42, 45-47 are formulated without a buffer and are not pH titrated.

金畔生物代理美国Hampton Research，负责其在中国的销售与推广，更多产品信息，欢迎与我们联系

Hampton Research HR2-082 PEGRx ，HR2-084 PEGRx 2

发表于由
Hampton Research HR2-082 PEGRx ，HR2-084 PEGRx 2

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商，其产品应用广泛且深受广大用户的认可。

PEGRx 1 • PEGRx 2 • PEGRx HT

HR2-082，PEGRx 1 10 ml, tube format

HR2-084 ，PEGRx 2 10 ml, tube format

HR2-086 ，PEGRx HT 1 ml, Deep Well block format

APPLICATIONS

Primary and secondary, polymer and pH based crystallization screen for biological macromolecules


FEATURES

Developed at Hampton Research

PEGRx 1 primary screen variables are polymer type (16 different polymers), polymer molecular weight, pH and low ionic strength.

PEGRx 2 primary screen variables are polymer type (13 different polymers), polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.

PEGRx HT combines PEGRx 1 and PEGRx 2 into a single 96 Deep Well block

pH range 3.5 – 9, using 10 different buffer systems

Polymer molecular weight range 200 to 20,000

Ligand friendly, complex friendly


DESCRIPTION

PEGRx is a primary and secondary, polymer and pH based crystallization screen developed at Hampton Research.

It is designed to evaluate polymer based crystallization reagents and pH in low (PEGRx 1) to medium ionic strength (PEGRx 2). It is also designed for use as a secondary or optimization screen to follow the Hampton Research Index screen when polymer based reagents produce hits and interesting solubility leads.

PEGRx 1 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a low ionic strength environment versus a wide range of pH. Polymer reagents include Polyethylene glycols, Polyethylene glycol monomethyl ethers, and Jeffamines®. The molecular weight range between 200 and 20,000 is evaluated in a low ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and low ionic strength.

PEGRx 2 is a crystallization reagent kit designed to evaluate an array of polymers of varying molecular weight in a medium ionic strength environment in the presence of additives, salts, volatile organics and polyols versus a wide range of pH. Polymer reagents include Polyethylene glycols and Polyethylene glycol monomethyl ethers. The polymer molecular weight range between 200 and 20,000 is evaluated in a medium ionic strength formulation. Ten different buffers are used to span the range of pH between 3.5 and 9. The primary screen variables are polymer type, polymer molecular weight, pH and secondary reagents which include additives, salts, volatile organics and polyols.


金畔生物代理美国Hampton Research，负责其在中国的销售与推广，更多产品信息，欢迎与我们联系

Hampton Research HR2-107 SaltRx 1，HR2-109 SaltRx 2

发表于由
Hampton Research HR2-107 SaltRx 1，HR2-109 SaltRx 2

Hampton Research是世界权威的蛋白结晶试剂与耗材的生产商，其产品应用广泛且深受广大用户的认可。

SaltRx 1 • SaltRx 2 • SaltRx HT

HR2-107， SaltRx 1，10 ml, tube format

HR2-109，SaltRx 2 ，10 ml, tube format

HR2-136，SaltRx HT，1 ml, Deep Well block format

APPLICATIONS

Salt versus pH matrix crystallization screen

22 unique salts versus salt concentration and pH

Tube or Deep Well block format

Developed at Hampton Research

Individual reagents available through the Hampton Research Custom Shop


DESCRIPTION

SaltRx was developed by Hampton Research as a primary and secondary, salt and pH based crystallization screen for biological macromolecules.

Salt is the only primary crystallization reagent (precipitant) utilized. Based on a design of 96 conditions, the screen evaluates a broad portfolio of crystallization salts of varying concentration and pH. The selection, concentration, and pH of the salts were determined by data mining the BMCD1 and other crystallization databases, crystallization reports in the literature, and internal crystallization trials performed at Hampton Research. Based on this analysis, up to 35% of protein crystallizations involve salt as the primary crystallization reagent. SaltRx can be used as a primary crystallization screen when salt, ionic strength and pH are desired or suspected as appropriate crystallization variables. It is also useful as a secondary screen when salt only reagents/conditions from screens such as Index™, Crystal Screen™, and Grid Screen™ produce crystals and when further screening for additional salt conditions or optimization is desired. Since this screen does not contain volatile organics, it is compatible with microbatch, vapor diffusion, liquid and gel diffusion crystallization methods. SaltRx may also be used for dialysis crystallization in conjunction with Dialysis Buttons™.

The original SaltRx kit (HR2-108) contained 96 unique reagents, 10 ml each. This kit has been discontinued and converted into two 48 reagent based kits, SaltRx 1 and SaltRx 2, available separately, which when used together are identical to the original SaltRx.

金畔生物代理美国Hampton Research，负责其在中国的销售与推广，更多产品信息，欢迎与我们联系