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Hypoxyprobe, Inc. 是一家致力于为恶性和正常组织疾病的诊断和治疗提供工具的小型公司,其中氧调节受到干扰。该公司位于121米德尔塞克斯高速公路,Burlington,马萨诸塞州,美国01803,是一个派生的天然药物公司(NPI)的低氧探头分区。
UxyPosich公司起源于James Raleigh博士在北卡罗来纳大学教堂山分校放射肿瘤学系和NPI的合作,当时位于北卡罗莱纳的研究三角公园。
UNC ChanelHill和NPI的合作首先关注肿瘤缺氧的临床研究。NPI大规模制备吡咪唑盐酸盐,Raleh博士提供了抗体试剂,用于检测人肿瘤中PimoDim唑与低氧细胞的结合。
在狗、大鼠和小鼠的临床前研究中,作为一种低氧标记物,科学界在PimoDimo-HCl中产生了越来越大的兴趣,并且在2002 NPI中发布了一项五年*许可,以CimICON有限公司为动物使用的低氧探针试剂市场。在2006,预期需要一个扩展的低氧探针试剂线,NPI结束CimICON许可证,并建立低氧探针,公司开发一种增强的试剂盒,用于动物和临床研究。
该公司目前致力于开发基于吡咪唑核心结构的无创性低氧标记物和缺氧依赖性细胞毒素。
Hypoxyprobe™ Kit
(HPI Catalog # HP1-XXX)
Solid pimonidazole HCl (Hypoxyprobe™-1)
Anti-pimonidazole mouse IgG1 monoclonal antibody (MAb1) (60 micrograms/ml)
Applications: Immunochemical detection of cell and tissue hypoxia through immunofluorescence or immunoperoxidase staining, western blotting, or flow cytometry.
Quantities: a. Hypoxyprobe™ Kit contains 100 mg, 200 mg or 1000 mg of pimonidazole HCl. Typical doses are 60mg/kg body weight for small animal studies and 14mg/kg body weight for human studies b. 1.0 or 2.0 ml of exhausted supernatant from hybridoma clone 4.3.11.3 containing 60 micrograms/ml of an IgG1 mouse monoclonal antibody and 0.15% sodium azide. Optimal dilution of MAb1 is to be determined by investigators but a 1/50 dilution has been found to give strong immunostaining in mouse tumor tissue when combined with a secondary strategy comprised of biotin-conjugated F(ab’)2 antimouse secondary reagent, streptavidin peroxidase and peroxidase substrate. Note that alternative secondary strategies can be used.
Not supplied: Secondary reagents such as biotin conjugated F(ab’)2 antimouse secondary reagent, streptavidin peroxidase or standard IHC reagents including buffers, peroxidase substrate, etc.
Storage: a. Store pimonidazole HCl solid at room temperature in the dark.
b. Store the mouse monoclonal antibody (MAb1) frozen. Avoid repeated freeze thaw cycles by aliquoting antibody into small volumes for storage at -20 degrees C.