标签归档：jbscreen

Jena Bioscience结晶筛选试剂盒 JBScreen Detergents HTS CS-525

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Jena Bioscience结晶筛选试剂盒 JBScreen Detergents HTS CS-525

Jena Bioscience在结构学研究方面全面提供晶体筛选试剂盒、优化试剂、工具和各类耗材


JBScreen Detergents 2 CS-522

JBScreen Detergents 3 CS-523

JBScreen Detergents 4 CS-524

JBScreen Detergents HTS CS-525

Jena Bioscience结晶筛选试剂盒 JBScreen Detergents HTS CS-525

JBScreen Detergents contain 4 x 24 unique detergents that are compatible with most common crystallization reagents and are therefore perfectly suited for membrane protein solubilization:

  • Ionic detergents

  • Non-ionic detergents

  • Zwitterionic detergents

  • Non-detergent Sulfobetaines

  • Synthetic Lipids

JBScreen Detergents can be used throughout the protein purification process or can be added afterwards by dialysis or ion-exchange chromatography (detergent exchange). Detergent exchange can be vital for obtaining well-diffracting membrane-protein crystals [1].

JBScreen Detergents is also valuable for additive screening with detergents and detergent mixtures [2,3] in combination with the JBScreen Membrane. This combination will enable you to screen a broad range of detergents, while concentrating on the most successful crystallization conditions, making crystallization screening of membrane proteins much more efficient and less time consuming.









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JBScreen Membrane 1 – 4 CS-309 JBScreen Membrane HTS CS-310

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JBScreen Membrane 1 – 4 CS-309 JBScreen Membrane HTS CS-310

Jena Bioscience在结构学研究方面全面提供晶体筛选试剂盒、优化试剂、工具和各类耗材

JBScreen Membrane 1 CS-301L

JBScreen Membrane 2 CS-302L

JBScreen Membrane 3 CS-303L

JBScreen Membrane 4 CS-304L

JBScreen Membrane 1 – 4 CS-309

JBScreen Membrane HTS CS-310


JBScreen Membrane covers 96 of the most successful conditions for crystallization of membrane proteins. Each individual composition results from an extensive analysis of the crystallization conditions that have yielded membrane protein structures so far.

The JBScreen Membrane crystallization conditions are primarily ordered by type and concentration of the precipitant. This allows easy extraction of all relevant information for a straightforward refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. The subsequent fine tuning of preliminary hits will be much more efficient.

JBScreen Detergents perfectly complement JBScreen Membrane: This combination enables you to screen a broad range of detergents, while concentrating on the most successful crystallization conditions, making crystallization screening of membrane proteins much more efficient and less time consuming.

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Jena Bioscience JBScreen LCP CS-340 JBScreen LCP HTS CS-213L

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Jena Bioscience JBScreen LCP CS-340 JBScreen LCP HTS CS-213L

Jena Bioscience在结构学研究方面全面提供晶体筛选试剂盒、优化试剂、工具和各类耗材


JBScreen LCP CS-340 

JBScreen LCP HTS CS-213L


JBScreen LCP is a crystallization screen designed for efficient screening of crystallization conditions in the Lipidic Cubic Phase (LCP), which has become the method of choice for membrane protein crystallization in different types of LCP lipids.
The 96 conditions of JBScreen LCP result from data mining of 192 integral membrane proteins, that were successfully crystallized by the in meso method and have yielded structures [1].
The screen is ordered by type and concentration of the precipitant and is free of cacodylate.

Jena Bioscience JBScreen LCP CS-340 JBScreen LCP HTS CS-213L

Cartoon representation of the events proposed to take place during the crystallization of an integral membrane protein from the lipid cubic mesophase. Image from [1], used by courtesy of Prof. Martin Caffrey, Trinity College Dublin, Ireland.


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JBScreen Basic 1 – 4 CS-125 JBScreen Basic HTS CS-203L

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JBScreen Basic 1 – 4 CS-125 JBScreen Basic HTS CS-203L

Jena Bioscience在结构学研究方面全面提供晶体筛选试剂盒、优化试剂、工具和各类耗材

JBScreen Basic 1 CS-121

JBScreen Basic 2 CS-122

JBScreen Basic 3 CS-123

JBScreen Basic 4 CS-124

JBScreen Basic 1 – 4 CS-125

JBScreen Basic HTS CS-203L


Despite intensive research, the crystallization of biological macromolecules remains a process of trial and error. Nucleation and crystal growth are influenced by the interaction of many variables, such as temperature, pH, precipitant and salt concentration.
Testing all possible combinations would be too time consuming and would require enormous amounts of sample. One approach to find suitable crystallization conditions is the sparse-matrix method. This method involves screening with an intentional bias towards conditions which have been proven successful in the crystallization of biological macromolecules.

In 1991, Jancarik and Kim published 50 conditions, which were derived from previously crystallized proteins [1]. These and other conditions form the basis of the JBScreen Basic system [1,2]. However, JBScreen Basic is designed to fit the 24-well plate format and like in all other JBScreen crystallization kits, we abstained from the use of cacodylate buffers and replaced them with MES. JBScreen Basic contains 96 unique reagent mixtures for screening a wide range of pH and various salts and precipitants.


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Jena Bioscience JBScreen Classic JBScreen Classic HTS

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Jena Bioscience JBScreen Classic JBScreen Classic HTS

Jena Bioscience在结构学研究方面全面提供晶体筛选试剂盒、优化试剂、工具和各类耗材


JBScreen Classic 1 CS-101L

JBScreen Classic 2 CS-102L

JBScreen Classic 3 CS-103L

JBScreen Classic 4 CS-104L

JBScreen Classic 5 CS-105L

JBScreen Classic 6 CS-106L

JBScreen Classic 7 CS-107

JBScreen Classic 8 CS-108L

JBScreen Classic 9 CS-109L

JBScreen Classic 10 CS-110L

JBScreen Classic 1 – 5 CS-112L

JBScreen Classic 6 – 10 CS-113L

JBScreen Classic 1 – 10 CS-114L

JBScreen Classic HTS I CS-201L

JBScreen Classic HTS II CS-202L

Jena Bioscience JBScreen Classic JBScreen Classic HTS

JBScreen Classic is a crystallization kit designed for efficient and flexible screening of crystallization conditions for proteins, peptides, nucleic acids, macromolecular complexes and water-soluble small molecules.

The JBScreen Classic Kits 1-10 cover 240 of the most prominent buffers for protein crystallization. Their compositions result from data mining of several thousands of crystallized proteins. JBScreen Classic represents the statistically most successful buffers that yielded protein crystals suitable for X-ray diffraction.

The JBScreen Classic buffers are principally ordered by type and concentration of the precipitant. This allows easy extraction of all relevant information and is already a first step to a refinement: Once you get a hit, you immediately see the effects of the neighbouring conditions. Subsequent fine tuning of preliminary hits will be much more efficient.

JBScreen Classic comprises 10 kits of 24 unique reagents in the standard 10 ml bulk format.

JBScreen Classic HTS I+II contains the formulations of the JBScreen system, adopted to fit the 96-well format for high throughput crystallization applications. Each JBScreen Classic HTS deep-well block is pre-filled with 96 sterile conditions at 1.7 ml each.












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