世界*实验材料供应商 Lab on a Bead AB 上海金畔生物为其中国代理, Lab on a Bead AB 在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品,的服务, Lab on a Bead AB 就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获
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Bead AB实验室是一家瑞典生物技术公司,专注于研究和开发下一代高容量磁选分离平台,用于从低微升实验室规模纯化生物分子至高浓度的制备规模。
Bead AB的实验室由BjörnStrandwitz和Sven Oscarsson于2007年成立。不久之后,瑞典乌普萨拉大学的Angstrom实验室,该公司也在四个化学和磁性领域的研究人员中长大。今天,研发机构位于乌普萨拉商业园区。
LOABeads蛋白A
高容量蛋白A磁性琼脂糖珠使用磁选法提供快速和简单的抗体纯化。
LOABeads蛋白A具有60mg人或兔IgG / ml沉降磁珠的结合能力。黑色珠子清晰可见,容易吸引到外部磁体上,使得在几秒钟内能够进行明显的分离,从而导致zui小的样品损失。4%琼脂糖基质提供zui小的非特异性结合,并使珠子不粘附,使珠粒容易分散和重新悬浮而不使用去污剂。
净化设置可以轻松扩大或缩小,以匹配抗体浓度和样品量。LOABeads蛋白A可以使用合适的磁选机(如LOABeads MagSep系列),从微升到升级分离。
LOABeads蛋白A用于从血清和腹水液样品中纯化抗体,IgG血清消耗和免疫沉淀。该产品仅供研究使用,不适合人类使用或用于诊断应用。
LOABeads Protein A
High capacity protein A magnetic agarose beads provide rapid and simple purification of antibodies using magnetic separation techniques.
LOABeads Protein A have a binding capacity 60 mg human or rabbit IgG per ml settled magnetic beads. The black beads are clearly visible and easily attracted to external magnets, enabling distinct separation within seconds, which leads to minimal sample loss. The 4% agarose matrix offers minimal unspecific binding and makes the beads non-adherent, letting the beads be easily dispersed and resuspended without the use of detergents.
The purification setup can easily be scaled up or down to match antibody concentration and sample volumes. LOABeads Protein A work excellently for separations from microliter to liter scales using suitable magnetic separators, such as the LOABeads MagSep series.
LOABeads Protein A are intended for purification of antibodies from serum and ascites fluid samples, IgG serum depletion, and immunoprecipitation. The product is for research use only and not intended for human use or for use in diagnostic applications.
Material Supplied
- LOABeads Protein A supplied as a 10% bead suspension in PBS with 20% ethanol.
1 ml of 10% bead suspension contains 100 µl magnetic beads.
- Neodymium cube magnet (12 mm) suitable for separations in 0.5-5 ml volumes.
Product Data
Product name |
LOABeads™ Protein A |
Product number |
1001 |
Particle size |
45–165 µm |
Ligand |
Recombinant protein A |
Type magnetization |
Super-paramagnetic |
Matrix |
4% agarose |
Product form |
10% bead suspension in PBS with 20% ethanol |
Binding capacity* |
4 mg rabbit IgG/ml bead suspension |
Max. binding capacity** |
6 mg human IgG/ml bead suspension |
Binding buffer |
PBS |
Elution buffer |
60 mM citrate pH 3.0 |
Storage conditions |
+2 to +8°C in PBS with 20% ethanol |
Stability information*** |
24 months |
Regeneration |
Can be reused multiple times |
* 90% binding of rabbit IgG (2 mg/ml) after 60 min reaction.
** Determined in an overloading test at 5 mg IgG/ml after 60 min reaction.
*** Data of product stability is continuously updated based on ongoing stability studies.
Ordering Information
Product |
Quantity |
Product no. |
LOABeads Protein A |
2×1 ml 10% beads |
1001-2ml |
LOABeads Protein A |
5×1 ml 10% beads |
1001-5m |
LOABeads Protein A |
10 ml 10% beads |
1001-10ml |
LOABeads AffiAmino
用于偶联含氨基配体的活性磁珠。
LOABeads AffiAmino是使用珠子耦合化学实验室的表面活化磁性琼脂糖珠。任何含氨基的配体,例如蛋白质和抗体可以使用简单的方法与珠共价结合。所有步骤均在生理条件和室温下进行,不使用任何危险试剂或有机溶剂。配体结合后,磁珠可用于亲和纯化和免疫沉淀。
LOABeads AffiAmino的结合能力为0.5-1mg人IgG / ml珠悬浮液(5-10mg / ml沉降珠),0.5-0.75mg蛋白A / ml珠悬浮液(5-7.5mg / ml沉降珠)和相似量的其他蛋白质。该键非常稳定(蛋白A的6ppm泄漏),并且珠可与结合的配体一起储存。
黑色珠子清晰可见,容易吸引到外部磁体上,使得在几秒钟内能够进行明显的分离,从而导致zui小的样品损失。4%琼脂糖基质提供zui小的非特异性结合,并使珠子不粘附,使珠粒容易分散和重新悬浮而不使用去污剂。
净化设置可以轻松扩大或缩小,以匹配抗体浓度和样品量。LOABeads AffiAmino使用合适的磁选机,例如LOABeads MagSep系列,可以很好的从微升到升级的分离。
供应材料
- LOABeads AffiAmino作为10%珠悬浮液在含20%乙醇的PBS中提供。
- 激活缓冲区
- 钕立方磁铁(12毫米),适用于0.5-5毫升体积分离。
产品资料
产品名称 |
LOABeads™AffiAmino |
产品编号 |
1003 |
粒度大小 |
45-165微米 |
耦合到 |
伯氨基 |
型磁化 |
超顺磁性 |
矩阵 |
4%琼脂糖 |
产品形式 |
10%珠悬浮液在含20%乙醇的PBS中 |
耦合能力* |
0.5-1mg IgG / ml珠悬浮液
5-10mg IgG / ml沉淀珠
|
绑定缓冲区 |
PBS含0.1%吐温 |
储存条件 |
在含20%乙醇的PBS中+2至+ 8℃ |
稳定性信息** |
12个月 |
*孵育60分钟后,兔IgG(1 mg / ml)的90%偶联。
**根据持续的稳定性研究,产品稳定性数据不断更新。
订购信息
产品 |
数量 |
产品编号 |
LOABeads AffiAmino |
2x1ml 10%珠 |
1003-2ml |
LOABeads AffiAmino |
5×1ml 10%珠 |
1003-5m |
LOABeads AffiAmino |
10毫升10%珠 |
1003-10ml |