上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
K. lactis GG799 Competent Cells
Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC1-based expression vector. GG799 cells are characterized by very high cell density growth and efficient expression of foreign proteins.
Sterile – competent cells are free of bacterial and fungal contamination.
Verified Transformation Efficiency – competent cells are tested for transformation efficiency as defined by the number of colony forming units (cfu) produced by transforming 1 μg of plasmid into a given volume of competent cells.
Optimal stability and efficiency if stored at -80C for up to 12 months.
GG799 cells have no genetic modifications.
GG799 is the basic strain provided in the K. lactis Protein Expression Kit (NEB# E1000S)
NEB does not add a dry ice surcharge to our competent cell shipments.
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
K. lactis Protein Expression Kit
The K. lactis Protein Expression Kit provides an easy method for expressing a gene of interest in the yeast Kluyveromyces lactis. Proteins may be produced intracellularly or secreted using an integrative expression vector (pKLAC2). To achieve protein secretion, a gene of interest is cloned downstream of the K. lactis α-mating factor secretion domain, which is eventually processed in the Golgi and results in secretion of the desired protein.
Supplied with competent K. lactis cells (NEB# C1001)
Allows cloning and expression of genes toxic to E. coli
Rapid high cell density growth results in high yield protein expression
Easy-to-use protocols for those inexperienced with yeast systems