标签归档：ligase

T4 DNA Ligase Reaction Buffer |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T4 DNA Ligase Reaction Buffer

T4 DNA Ligase is the industry standard for performance and quality.

At a 1X concentration this reaction buffer assures optimal activity of the enzyme. 

精选视频

T4 DNA Ligase Reaction Buffer |

Quick Tips – Why is there precipitate in my T4 DNA ligase buffer?

观看其他视频

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
B0202S 10 X 5 ml ¥359.00 有 无 有 无 无
如何订购
Please enter a quantity for at least one size

T4 DNA Ligase Reaction Buffer | Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
Learn More

库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 B0202S 10 X 5 ml ¥359.00 有 无 有 无 无

T4 RNA Ligase 1 (ssRNA Ligase) |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T4 RNA Ligase 1 (ssRNA Ligase)

There is also a high concentration (30,000 units/ml) version of this enzyme available, T4 RNA Ligase 1 (ssRNA Ligase), High Concentration (NEB #M0437M).

T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.

精选视频

T4 RNA Ligase 1 (ssRNA Ligase) |

Avoiding RNase Contamination

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0204S 10,000 units/ml 1,000 units ¥729.00 有 有 有 有 有
M0204L 10,000 units/ml 5,000 units ¥2,939.00 有 有 有 有 有
如何订购
Please enter a quantity for at least one size

T4 RNA Ligase 1 (ssRNA Ligase) | Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
Learn More

库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0204S 10,000 units/ml 1,000 units ¥729.00 有 有 有 有 有 M0204L 10,000 units/ml 5,000 units ¥2,939.00 有 有 有 有 有

T4 RNA Ligase 1 (ssRNA Ligase), High Concentration |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T4 RNA Ligase 1 (ssRNA Ligase), High Concentration

There is also a regular concentration (10,000 units/ml) version of this enzyme available, T4 RNA Ligase 1 (ssRNA Ligase) (NEB #M0204).

T4 RNA Ligase 1 catalyzes the ligation of a 5´ phosphoryl-terminated nucleic acid donor to a 3´ hydroxyl-terminated nucleic acid acceptor through the formation of a 3´ → 5´ phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.

精选视频

T4 RNA Ligase 1 (ssRNA Ligase), High Concentration |

Avoiding RNase Contamination

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0437M 30,000 units/ml 5,000 units ¥3,129.00 有 有 有 无 有
如何订购
Please enter a quantity for at least one size

库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0437M 30,000 units/ml 5,000 units ¥3,129.00 有 有 有 无 有

T4 RNA Ligase 2 (dsRNA Ligase) |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T4 RNA Ligase 2 (dsRNA Ligase)

T4 RNA Ligase 2 ligates nicks in dsRNA and can also be used to ligate the 3´ OH of RNA to the 5´ phosphate of DNA in a double stranded structure.

  • Best choice for ligating nicks in dsRNA
  • Suitable for ligating 3′ OH of RNA to 5′ phosphate of DNA in a DNA/RNA hybrid
  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of endonucleases, exonucleases and RNases
  • Not sure which ligase to choose? Refer to our DNA and RNA Ligase Properties Chart 

精选视频

T4 RNA Ligase 2 (dsRNA Ligase) |

Avoiding RNase Contamination

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0239S 10,000 units/ml 150 units ¥889.00 有 无 无 无 无
M0239L 10,000 units/ml 750 units ¥3,549.00 无 无 无 无 有
如何订购
Please enter a quantity for at least one size

T4 RNA Ligase 2 (dsRNA Ligase) | Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
Learn More

库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0239S 10,000 units/ml 150 units ¥889.00 有 无 无 无 无 M0239L 10,000 units/ml 750 units ¥3,549.00 无 无 无 无 有

T4 RNA Ligase 2, truncated |NEB酶试剂 New England Biolabs

发表于由

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

T4 RNA Ligase 2, truncated

  • T4 RNA Ligase 2, truncated (T4 Rnl2tr) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.
  • The enzyme does not require ATP for ligation but does need the pre-adenylated substrate.
  • T4 Rnl2tr is expressed from a plasmid in E. coli, which encodes the first 249 amino acids of the full length T4 RNA Ligase 2.
  • Unlike the full length ligase, T4 Rnl2tr cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA.
  • This enzyme, also known as Rnl2 (1–249) has been used for optimized linker ligation for the cloning of microRNAs.
  • This enzyme reduces background ligation because it can only use pre-adenylated linkers.

精选视频

T4 RNA Ligase 2, truncated |

Avoiding RNase Contamination

库存
货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0242S 200,000 units/ml 2,000 units ¥799.00 有 无 无 无 无
M0242L 200,000 units/ml 10,000 units ¥3,219.00 有 有 无 无 无
如何订购
Please enter a quantity for at least one size

T4 RNA Ligase 2, truncated | Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
Learn More

库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0242S 200,000 units/ml 2,000 units ¥799.00 有 无 无 无 无 M0242L 200,000 units/ml 10,000 units ¥3,219.00 有 有 无 无 无