上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG
Supplied as a lyophilized cake, the LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG enables sensitive detection of target RNA sequences in a room temperature-stable format. This product contains the same versatile features and strong performance as the liquid version: Luna® Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019).
- Simply add nuclease-free water for rapid rehydration (view protocol)
- Store at room temperature for up to 2 years prior to rehydration
- Eliminate cold chain shipping requirements
- Multiplex up to 5 targets to increase throughput
- Increase reaction specificity and robustness with our unique pairing of Luna WarmStart® RT and Hot Start Taq
- Prevent carryover contamination with Thermolabile UDG and dUTP included in optimized mix
- Maintain RNA integrity with Murine RNase Inhibitor included in optimized mix
- Eliminate pipetting errors with non-fluorescent blue tracking dye
- Use with a variety of instrument platforms, including those that require a high or low ROX reference signal
- Developed jointly by New England Biolabs and Fluorogenics™ Limited, which is now a subsidiary of New England Biolabs, Inc.
精选视频
LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG
Need a custom/large volume order? Contact us
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
The mix contains the necessary components for one-step RT-qPCR, including Luna WarmStart RT, Hot Start Taq DNA Polymerase, dNTPs, and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start Taq DNA Polymerase with a WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps prevent undesirable non-specific priming and extension prior to thermocycling, enabling room temperature reaction set-up after rehydration. The engineered Luna WarmStart RT also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. Additionally, the mix is formulated with a unique passive reference dye that is compatible across a variety of instrument platforms, including those that require a high or low ROX reference signal.
The sensitivity of RT-qPCR makes it important to minimize DNA contamination wherever possible. The inclusion of dUTP and thermolabile UDG prevents carryover contamination, where a product of a previous amplification can serve as the substrate of a subsequent reaction. Unlike E.coli UDG, the thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on qPCR performance.
A non-fluorescent visible blue tracking dye is included to avoid pipetting errors. The tracking dye does not overlap spectrally with fluorophores commonly used in qPCR and does not interfere with real-time detection.
To learn about the lyophilization process, or to inquire about customizing this product to meet your assay needs, please visit www.neb.com/lyoprime.
- 产品类别:
- Luna® qPCR & RT-qPCR Products
- 应用:
- qPCR & RT-qPCR,
- DNA Amplification, PCR & qPCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
L4001S 15 to 25 LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG L4001SVIAL 15 to 25 2 x 60 reactions 不适用
-
- 特性和用法
- 优势和特性
Features
- Can be reconstituted at either 2X or 4X if desired to permit higher sample volumes
- Same versatile features and strong performance of the Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019) in a lyophilized format
- Includes aptamer technology to control RT and DNA polymerase enzyme activity
- 相关产品
相关产品
- Nuclease-free Water
- Luna® Probe One-Step RT-qPCR 4X Mix with UDG
- Luna® Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)
- Monarch® Total RNA Miniprep Kit
操作说明、说明书 & 用法
- 操作说明
- Protocol for LyoPrime Luna One-Step RT-qPCR Mix with UDG (NEB #L4001)
- 应用实例
- Key considerations for optimal lyophilized reagent development
- Optimized conditions for the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay using Luna One-Step RT-qPCR Reagents
FAQs & 问题解决指南
- FAQs
- How should the LyoPrime Luna mix be stored?
- How do I rehydrate the LyoPrime Luna mix? Can it be resuspended at a higher concentration?
- Are reaction conditions the same for lyophilized and liquid Luna mixes?
- When I dissolve the lyophilized product, the solution at first appears turbid. Is this normal?
- Can I set up my Luna® RT-qPCR at room temperature?
- Why is the Luna® qPCR Mix blue? Will this dye interfere with detection?
- Can I run the Luna® qPCR Mix on my qPCR instrument?
- Do I need to add ROX?
- What RNA samples can be used in RT-qPCR with the Luna® Mix?
- How much RNA template should I use in my RT-qPCR reaction?
- Can I run multiplex reactions with the Luna® Probe One-Step RT-qPCR Kits? Do I need to change my reaction conditions?
- Can I use a ROX-labeled probe with the Luna® Probe Mix that contain a reference dye?
- Other companies recommend a 2-min incubation at 25°C for carryover prevention, is a 30-sec incubation at 25°C sufficient?
- Can I use the Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019) product for viral detection?
- What are the differences between the Luna One-Step RT-qPCR products (NEB #E3006, #E3007 and #M3019)?
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- L4001S_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- L4001S_v1_10133633
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG
-
LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG
Supplied as a lyophilized cake, the LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG enables sensitive detection of target RNA sequences in a room temperature-stable format. This product contains the same versatile features and strong performance as the liquid version: Luna® Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019).
- Simply add nuclease-free water for rapid rehydration (view protocol)
- Store at room temperature for up to 2 years prior to rehydration
- Eliminate cold chain shipping requirements
- Multiplex up to 5 targets to increase throughput
- Increase reaction specificity and robustness with our unique pairing of Luna WarmStart® RT and Hot Start Taq
- Prevent carryover contamination with Thermolabile UDG and dUTP included in optimized mix
- Maintain RNA integrity with Murine RNase Inhibitor included in optimized mix
- Eliminate pipetting errors with non-fluorescent blue tracking dye
- Use with a variety of instrument platforms, including those that require a high or low ROX reference signal
- Developed jointly by New England Biolabs and Fluorogenics™ Limited, which is now a subsidiary of New England Biolabs, Inc.
精选视频
LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG
Need a custom/large volume order? Contact us
Bulk packaging may also be available and requested for large recurring orders.
Learn More
产品信息
The mix contains the necessary components for one-step RT-qPCR, including Luna WarmStart RT, Hot Start Taq DNA Polymerase, dNTPs, and Murine RNase Inhibitor in an optimized buffer. Combining Hot Start Taq DNA Polymerase with a WarmStart-activated reverse transcriptase allows for dual control of enzyme activity via reversible, aptamer-based inhibition. This temperature-dependent activation helps prevent undesirable non-specific priming and extension prior to thermocycling, enabling room temperature reaction set-up after rehydration. The engineered Luna WarmStart RT also possesses higher thermostability than many other RTs, allowing an optimal reaction temperature of 55°C. Additionally, the mix is formulated with a unique passive reference dye that is compatible across a variety of instrument platforms, including those that require a high or low ROX reference signal.
The sensitivity of RT-qPCR makes it important to minimize DNA contamination wherever possible. The inclusion of dUTP and thermolabile UDG prevents carryover contamination, where a product of a previous amplification can serve as the substrate of a subsequent reaction. Unlike E.coli UDG, the thermolabile UDG is completely inactivated at temperatures above 50°C, thereby having no effect on qPCR performance.
A non-fluorescent visible blue tracking dye is included to avoid pipetting errors. The tracking dye does not overlap spectrally with fluorophores commonly used in qPCR and does not interfere with real-time detection.
To learn about the lyophilization process, or to inquire about customizing this product to meet your assay needs, please visit www.neb.com/lyoprime.
- 产品类别:
- Luna® qPCR & RT-qPCR Products
- 应用:
- qPCR & RT-qPCR,
- DNA Amplification, PCR & qPCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
L4001S 15 to 25 LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG L4001SVIAL 15 to 25 2 x 60 reactions 不适用
-
- 特性和用法
- 优势和特性
Features
- Can be reconstituted at either 2X or 4X if desired to permit higher sample volumes
- Same versatile features and strong performance of the Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019) in a lyophilized format
- Includes aptamer technology to control RT and DNA polymerase enzyme activity
- 相关产品
相关产品
- Nuclease-free Water
- Luna® Probe One-Step RT-qPCR 4X Mix with UDG
- Luna® Probe One-Step RT-qPCR 4X Mix with UDG (No ROX)
- Monarch® Total RNA Miniprep Kit
操作说明、说明书 & 用法
- 操作说明
- Protocol for LyoPrime Luna One-Step RT-qPCR Mix with UDG (NEB #L4001)
- 应用实例
- Key considerations for optimal lyophilized reagent development
- Optimized conditions for the CDC Influenza SARS-CoV-2 (Flu SC2) Multiplex Assay using Luna One-Step RT-qPCR Reagents
FAQs & 问题解决指南
- FAQs
- How should the LyoPrime Luna mix be stored?
- How do I rehydrate the LyoPrime Luna mix? Can it be resuspended at a higher concentration?
- Are reaction conditions the same for lyophilized and liquid Luna mixes?
- When I dissolve the lyophilized product, the solution at first appears turbid. Is this normal?
- Can I set up my Luna® RT-qPCR at room temperature?
- Why is the Luna® qPCR Mix blue? Will this dye interfere with detection?
- Can I run the Luna® qPCR Mix on my qPCR instrument?
- Do I need to add ROX?
- What RNA samples can be used in RT-qPCR with the Luna® Mix?
- How much RNA template should I use in my RT-qPCR reaction?
- Can I run multiplex reactions with the Luna® Probe One-Step RT-qPCR Kits? Do I need to change my reaction conditions?
- Can I use a ROX-labeled probe with the Luna® Probe Mix that contain a reference dye?
- Other companies recommend a 2-min incubation at 25°C for carryover prevention, is a 30-sec incubation at 25°C sufficient?
- Can I use the Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019) product for viral detection?
- What are the differences between the Luna One-Step RT-qPCR products (NEB #E3006, #E3007 and #M3019)?
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- L4001S_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- L4001S_v1_10133633
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
LyoPrime Luna™ Probe One-Step RT-qPCR Mix with UDG
-