上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NheI
This product has been discontinued and is replaced with the High-Fidelity version, NheI-HF (NEB #R3131)
Catalog # R0131 was discontinued on March 17, 2021
同裂酶 | 单字母标示 | Pronunciation:
Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF®) version available (NEB #R3131) supplied with CutSmart® Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Cut Site: G/CTAGC
精选视频
Reduce Star Activity with High-Fidelity Restriction Enzymes
观看其他视频
产品信息
NheI has a High Fidelity version NheI-HF® (NEB #R3131).
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
产品来源
An E. coli strain that carries the NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999).
产品类别:
Discontinued Products
特性和用法
单位定义
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
反应条件
1X NEBuffer™ 2.1 Incubate at 37°C
1X NEBuffer™ 2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml BSA (pH 7.9 @ 25°C)
10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C
热失活
65°C for 20 min
甲基化敏感性
dam 甲基化: Not Sensitive dcm 甲基化: Not Sensitive CpG甲基化: Blocked by Some Combinations of Overlapping
相关产品
相关产品
NheI-HF®
Monarch® Plasmid Miniprep Kit
Monarch® DNA Gel Extraction Kit
Monarch® PCR & DNA Cleanup Kit (5 μg)
Nuclease-free Water
单独销售的组分
Gel Loading Dye, Purple (6X)
NEBuffer 2.1
注意事项
Cleaves to leave a 5´ CTAG extension which can be efficiently ligated to DNA fragments generated by AvrII, SpeI or XbaI.
Inhibited by salt concentrations > 100 mM.
This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
Blocked by some combinations of overlapping CpG methylation.
操作说明、说明书 & 用法
操作说明
Optimizing Restriction Endonuclease Reactions
Restriction Digest Protocol
Double Digest Protocol with Standard Restriction Enzymes
使用指南
Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
Activity of Restriction Enzymes in PCR Buffers
Cleavage Close to the End of DNA Fragments
Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
Double Digests
Effects of CpG Methylation on Restriction Enzyme Cleavage
Heat Inactivation
NEBuffer Activity/Performance Chart with Restriction Enzymes
Optimizing Restriction Endonuclease Reactions
Restriction Endonucleases – Survival in a Reaction
Restriction Enzyme Diluent Buffer Compatibility
Restriction Enzyme Tips
Single Letter Codes
Site Preferences
Star Activity
Traditional Cloning Quick Guide
工具 & 资源
选择指南
Alphabetized List of Recognition Sequences
Cleavage of Supercoiled DNA
Compatible Cohesive Ends and Generation of New Restriction Sites
Dam-Dcm and CpG Methylation
Frequencies of Restriction Sites
Isoelectric Points (pI) for Restriction Enzymes
Isoschizomers
NEB Diluent and Buffer Table
Recleavable Filled-in 5′ Overhangs
Time-Saver™ Qualified Enzymes
Why Choose Recombinant Enzymes?
Web 工具
Competitor Cross-Reference Tool
DNA Sequences and Maps Tool
Double Digest Finder
Enzyme Finder
NEBcutter™ v3.0
NEBioCalculator®
REBASE®
FAQs & 问题解决指南
FAQs
Is NheI affected by methylation?
Are more units of NheI required to cut supercoiled DNA than lambda DNA?
Is NheI inhibited by salt?
Is NheI active at 25°C?
Does NheI produce commonly used compatible ends?
Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
Why is my Restriction Enzyme not cutting DNA?
Why do I see a DNA smear on an agarose gel after a restriction digest?
Why do I see additional DNA bands on my gel after a restriction digest?
How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NheI
This product has been discontinued and is replaced with the High-Fidelity version, NheI-HF (NEB #R3131)
Catalog # R0131 was discontinued on March 17, 2021
同裂酶 | 单字母标示 | Pronunciation:
Time-Saver™ qualified for digestion in 5-15 minutes
High Fidelity (HF®) version available (NEB #R3131) supplied with CutSmart® Buffer
Supplied with 1 vial of Gel Loading Dye, Purple (6X)
Cut Site: G/CTAGC
精选视频
Reduce Star Activity with High-Fidelity Restriction Enzymes
观看其他视频
产品信息
NheI has a High Fidelity version NheI-HF® (NEB #R3131).
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
产品来源
An E. coli strain that carries the NheI gene from Neisseria mucosa heidelbergensis (ATCC 25999).
产品类别:
Discontinued Products
特性和用法
单位定义
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl.
反应条件
1X NEBuffer™ 2.1 Incubate at 37°C
1X NEBuffer™ 2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml BSA (pH 7.9 @ 25°C)
10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C
热失活
65°C for 20 min
甲基化敏感性
dam 甲基化: Not Sensitive dcm 甲基化: Not Sensitive CpG甲基化: Blocked by Some Combinations of Overlapping
相关产品
相关产品
NheI-HF®
Monarch® Plasmid Miniprep Kit
Monarch® DNA Gel Extraction Kit
Monarch® PCR & DNA Cleanup Kit (5 μg)
Nuclease-free Water
单独销售的组分
Gel Loading Dye, Purple (6X)
NEBuffer 2.1
注意事项
Cleaves to leave a 5´ CTAG extension which can be efficiently ligated to DNA fragments generated by AvrII, SpeI or XbaI.
Inhibited by salt concentrations > 100 mM.
This enzyme has shown to have lower activity on some supercoiled plasmids, with more than 1 unit required to digest 1 μg plasmid DNA. For complete digestion of 1 μg of plasmid DNA please follow our recommended digestion protocol.
Blocked by some combinations of overlapping CpG methylation.
操作说明、说明书 & 用法
操作说明
Optimizing Restriction Endonuclease Reactions
Restriction Digest Protocol
Double Digest Protocol with Standard Restriction Enzymes
使用指南
Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures
Activity of Restriction Enzymes in PCR Buffers
Cleavage Close to the End of DNA Fragments
Digestion of Agarose-Embedded DNA: Info for Specific Enzymes
Double Digests
Effects of CpG Methylation on Restriction Enzyme Cleavage
Heat Inactivation
NEBuffer Activity/Performance Chart with Restriction Enzymes
Optimizing Restriction Endonuclease Reactions
Restriction Endonucleases – Survival in a Reaction
Restriction Enzyme Diluent Buffer Compatibility
Restriction Enzyme Tips
Single Letter Codes
Site Preferences
Star Activity
Traditional Cloning Quick Guide
工具 & 资源
选择指南
Alphabetized List of Recognition Sequences
Cleavage of Supercoiled DNA
Compatible Cohesive Ends and Generation of New Restriction Sites
Dam-Dcm and CpG Methylation
Frequencies of Restriction Sites
Isoelectric Points (pI) for Restriction Enzymes
Isoschizomers
NEB Diluent and Buffer Table
Recleavable Filled-in 5′ Overhangs
Time-Saver™ Qualified Enzymes
Why Choose Recombinant Enzymes?
Web 工具
Competitor Cross-Reference Tool
DNA Sequences and Maps Tool
Double Digest Finder
Enzyme Finder
NEBcutter™ v3.0
NEBioCalculator®
REBASE®
FAQs & 问题解决指南
FAQs
Is NheI affected by methylation?
Are more units of NheI required to cut supercoiled DNA than lambda DNA?
Is NheI inhibited by salt?
Is NheI active at 25°C?
Does NheI produce commonly used compatible ends?
Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer?
Why is my Restriction Enzyme not cutting DNA?
Why do I see a DNA smear on an agarose gel after a restriction digest?
Why do I see additional DNA bands on my gel after a restriction digest?
How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
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