上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Nuclease-free Water
- Ideal for the preparation of reagents and for use in enzymatic reactions
- No toxic agents, such as DEPC, are used in production, so as to avoid inhibition in enzymatic reactions
- Available in 25 ml and 100 ml sizes
Micrococcal Nuclease |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Micrococcal Nuclease
- DNA and RNA endonuclease
- Degrades double-stranded and single-stranded DNA and RNA
- Products are mononucleotides and oligonucleotides with terminal 3′-phosphates
Micrococcal Nuclease is ideal for:
- Preparation of double-stranded DNA fragments with 5′-OH and 3′-phosphate
- Studies of chromatin structure
- Degradation of nucleic acids in crude cell-free extracts
- Preparation of rabbit reticulocyte
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Nuclease P1 |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Nuclease P1
- Single-stranded (ssDNA or RNA) specific endonuclease
Nuclease P1 is ideal for:
- Conversion of single-stranded DNA or RNA to 5′-mononucleotides
- Analysis of the base composition of nucleic acids
- Studies of the potential damage and modification in DNA
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Need a custom/large volume order? Contact Us
Bulk packaging may also be available and requested for large recurring orders.
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Nuclease BAL-31 | NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Nuclease BAL-31
Please contact [email protected] to discuss alternatives.
- Catalog # M0213 was discontinued on November 09, 2021
- DNA and RNA nuclease
- Degrades linear double-stranded DNA from both 5′ and 3′ termini
- Acts as an endonuclease that cleaves at nicks, gaps, and single-stranded regions of double-stranded DNA and RNA
Nuclease BAL-31 is ideal for:
- Progressive shortening of double-stranded DNA
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产品信息
B) Lambda DNA-HaeIII digest after 2 minute incubation with one unit of Nuclease BAL-31.
C) As in (B) followed by incubation with T4 DNA Ligase.
产品来源
Purified from the culture medium of Alteromonas espejiana BAL-31. Contains a mixture of “fast” and “slow” species of the enzyme (3).
- 产品类别:
- Discontinued Products
- 特性和用法
单位定义
One unit is defined as the amount of enzyme required to remove 200 base pairs from each end of linearized double-stranded ΦX174 DNA (40 µg/ml) in a total reaction volume of 50 μl in 10 minutes at 30°C in 1X Nuclease BAL-31 Reaction Buffer.
反应条件
1X Nuclease BAL-31 Reaction Buffer
Incubate at 30°C1X Nuclease BAL-31 Reaction Buffer
20 mM Tris-HCl
600 mM NaCl
12 mM MgCl2
12 mM CaCl2
1 mM EDTA
(pH 8 @ 25°C)贮存溶液
0.25 mM EDTA
10 mM Tris-HCl
50 mM NaCl
1.5 mM CaCl2
1.5 mM MgCl2
200 µg/ml BSA
50% Glycerol
pH 8 @ 25°C热失活
65°C for 20 min
in the presence of 30mM EGTA
- 优势和特性
应用特性
- Progressive shortening of double-stranded DNA fragments at both termini (4)
- Restriction site mapping (2)
- 相关产品
相关产品
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
- 注意事项
- Duplex products of the exonuclease are a mixture of blunt and staggered ends. This mixture can be cloned directly, although maximal ligation efficiency requires repairing the staggered ends with a suitable DNA polymerase.
- If necessary, the enzyme may be diluted in reaction buffer prior to use.
- Activity is linear with enzyme concentration.
- Heat Inactivation will only work in the presence of 30mM EGTA.
- 参考文献
- Gray, H.B. et al. (1975). Nucl.Acids Res.. 2, 1459-1492.
- Legerski, R.J. et al. (1978). Nucl.Acids Res.. 5, 1445-1463.
- Wei, C.-F. et al. (1983). J. Biol.Chem.. 258, 13506-13512.
- Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual(2nd Ed.). 5.73-5.75.
操作说明、说明书 & 用法
- 操作说明
- Protocol for Nuclease BAL-31
工具 & 资源
- Web 工具
- Exo Selector
FAQs & 问题解决指南
- FAQs
- Can Nuclease BAL-31 be used to remove 10 base pairs from the end of a DNA fragment?
- Why does all of the DNA get degraded when I use Nuclease BAL-31?
- Can Nuclease BAL-31 be heat inactivated?
- Is Nuclease BAL-31 active in other NEBuffers?
- Can Nuclease BAL-31 treated DNA be cloned?
- What is a good control for the BAL-31 nuclease?
- Will Nuclease BAL-31 degrade RNA?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
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质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- M0213S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- M0213S_v0_10016795
- M0213S_v0_10034567
- M0213S_v1_10036382
- M0213S_v1_10049151
- M0213S_v1_10084119
- M0213S_v1_10109043
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
Nuclease BAL-31
-