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Next® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 2) |NEB酶试剂 New England Biolabs

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NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 2)

NEBNext® Multiplex Oligos for Illumina® are an essential component of the NEBNext suite of library preparation products, and are available in several different configurations for use with NEBNext products or other standard Illumina-compatible library preparation protocols. This set was designed and optimized for use in DNA sequencing workflows, including PCR-free. These unique dual index adaptors also include a 12-nt unique molecular identifier (UMI) sequence to enable either the PCR-free sample preparation for NGS, or the identification and removal of PCR errors or duplicates from amplified libraries. Supplied as 96 unique, pre-annealed index adaptors with UMIs, packaged in a single-use 96-well plate with a pierceable foil seal. Universal primers are included for applications requiring PCR amplification.

Additional NEBNext Unique Dual Index UMI Adaptors for DNA-seq are available (NEB #E7395, #E7876, #E7878) for multiplexing up to 384 samples. NEBNext Unique Dual Index UMI Adaptors for RNA-seq (NEB # E7416) are also available.

  • Index adaptors enable PCR-free library preparation
  • Increased sample identification specificity
  • Superior and reliable ligation efficiency
  • More-sensitive detection of low-frequency single-nucleotide variants (SNVs)
  • Supports multiplexing for higher throughputs