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Hypoxyprobe™ Omni Kit
HPI Catalog # HP3-XXX
Kit contents: Solid pimonidazole HCl (Hypoxyprobe™-1) and affinity purified rabbit anti- pimonidazole antibody (PAb2627AP)(ca 0.5 mg/mL IgG)
Applications: Immunochemical detection of cell and tissue hypoxia through immunofluorescence or immunoperoxidase staining, western blotting, or flow cytometry. Very low background in mouse tissues when the Hypoxyprobe™-1 Omni Kit is combined with an anti-rabbit secondary reagent (secondary reagent not supplied in the Omni Kit).
Quantities: a. Hypoxyprobe™-1 Omni Kits contain100 mg, 200 mg or 1000 mg of Hypoxyprobe™- 1 (pimonidazole HCl). Typical doses are 60mg/kg body weight for small animal studies and 14mg/kg body weight for human studies
b. One vial (100 and 200 mg Kits) or two vials (1000 mg Kit) of 200 microliters of affinity purified anti-pimonidazole rabbit antisera containing 0.09% sodium azide and 1% BSA as stabilizers. Optimal dilution of PAb2627AP is to be determined by each investigator but a 1/50 -1/200 dilution has been found to give strong immunostaining in mouse tumor tissue when combined with peroxidase conjugated goat anti-rabbit antisera.
Not supplied: Secondary anti-rabbit reagent and standard IHC reagents such as buffers, substrate chromogens, etc.
Storage: a. Store Hypoxyprobe™-1 solid at room temperature in the dark (can also be stored at 2- 8 degrees C).
b. Store rabbit PAb2627AP at 2-8 degrees C. Do not freeze
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