PhyNexus 2017报价

发表于2024年11月1日由Amresco中国官网

世界*实验材料供应商 PhyNexus上海金畔生物为其中国代理， PhyNexus在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， PhyNexus就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

PhyNexus中国代理, PhyNexus上海代理， PhyNexus北京代理，PhyNexus广东代理， PhyNexus江苏代理PhyNexus湖北代理,PhyNexus天津,PhyNexus黑龙江代理,PhyNexus内蒙古代理,PhyNexus吉林代理,PhyNexus福建代理, PhyNexus江苏代理, PhyNexus浙江代理, PhyNexus四川代理,

Douglas Gjerde于2002年在加利福尼亚州的圣何塞市建立了PhyNexus公司，以调查蛋白质样品制备的需求。专栏和机器人被开发小型化和自动化过程，当保留蛋白质的自然状态时。2005年，引进了一整列的专栏和机器人。在接下来的几年中，柱子被修改为与所有主要的机器人液体处理器兼容。定期提供超过20种色谱柱的化学品，提供超过100种定制包装的产品。从2016年开始，引入了试剂盒和仪器，大大增加了瞬时转染质粒的可用性。PhyNexus客户是制药公司和学术界的生命科学家。

部分产品报价如下：

货号	品名	规格	目录价	品牌
PTR 41-10-01	PhyTip columns (1 mL volume) containing 10 µL of Protein A affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-01	PhyTip columns (1 mL volume) containing 20 µL of Protein A affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-01	PhyTip columns (1 mL volume) containing 40 µL of Protein A affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-01	PhyTip columns (1 mL volume) containing 80 µL of Protein A affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-01	PhyTip columns (1 mL volume) containing 160 µL of Protein A affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-01	PhyTip columns (1 mL volume) containing 320 µL of Protein A affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-01	PhyTip columns (1 mL volume) containing 10 µL of Protein A affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-01	PhyTip columns (1 mL volume) containing 20 µL of Protein A affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-01	PhyTip columns (1 mL volume) containing 40 µL of Protein A affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-01	PhyTip columns (1 mL volume) containing 80 µL of Protein A affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-01	PhyTip columns (1 mL volume) containing 160 µL of Protein A affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-01	PhyTip columns (1 mL volume) containing 320 µL of Protein A affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-02	PhyTip columns (1 mL volume) containing 10 µL of Protein G affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-02	PhyTip columns (1 mL volume) containing 20 µL of Protein G affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-02	PhyTip columns (1 mL volume) containing 40 µL of Protein G affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-02	PhyTip columns (1 mL volume) containing 80 µL of Protein G affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-02	PhyTip columns (1 mL volume) containing 160 µL of Protein G affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-02	PhyTip columns (1 mL volume) containing 320 µL of Protein G affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-02	PhyTip columns (1 mL volume) containing 10 µL of Protein G affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-02	PhyTip columns (1 mL volume) containing 20 µL of Protein G affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-02	PhyTip columns (1 mL volume) containing 40 µL of Protein G affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-02	PhyTip columns (1 mL volume) containing 80 µL of Protein G affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-02	PhyTip columns (1 mL volume) containing 160 µL of Protein G affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-02	PhyTip columns (1 mL volume) containing 320 µL of Protein G affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-03	PhyTip columns (1 mL volume) containing 10 µL of IMAC affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-03	PhyTip columns (1 mL volume) containing 20 µL of IMAC affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-03	PhyTip columns (1 mL volume) containing 40 µL of IMAC affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-03	PhyTip columns (1 mL volume) containing 80 µL of IMAC affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-03	PhyTip columns (1 mL volume) containing 160 µL of IMAC affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-03	PhyTip columns (1 mL volume) containing 320 µL of IMAC affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-03	PhyTip columns (1 mL volume) containing 10 µL of IMAC affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-03	PhyTip columns (1 mL volume) containing 20 µL of IMAC affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-03	PhyTip columns (1 mL volume) containing 40 µL of IMAC affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-03	PhyTip columns (1 mL volume) containing 80 µL of IMAC affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-03	PhyTip columns (1 mL volume) containing 160 µL of IMAC affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-03	PhyTip columns (1 mL volume) containing 320 µL of IMAC affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-04	PhyTip columns (1 mL volume) containing 10 µL of Glutathione affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-04	PhyTip columns (1 mL volume) containing 20 µL of Glutathione affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-04	PhyTip columns (1 mL volume) containing 40 µL of Glutathione affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-04	PhyTip columns (1 mL volume) containing 80 µL of Glutathione affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-04	PhyTip columns (1 mL volume) containing 160 µL of Glutathione affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-04	PhyTip columns (1 mL volume) containing 320 µL of Glutathione affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-04	PhyTip columns (1 mL volume) containing 10 µL of Glutathione affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-04	PhyTip columns (1 mL volume) containing 20 µL of Glutathione affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-04	PhyTip columns (1 mL volume) containing 40 µL of Glutathione affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-04	PhyTip columns (1 mL volume) containing 80 µL of Glutathione affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-04	PhyTip columns (1 mL volume) containing 160 µL of Glutathione affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-04	PhyTip columns (1 mL volume) containing 320 µL of Glutathione affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-05	PhyTip columns (1 mL volume) containing 10 µL of Streptavidin per column	Box of 48	9180	PhyNexus
PTR 41-20-05	PhyTip columns (1 mL volume) containing 20 µL of Streptavidin per column	Box of 48	13056	PhyNexus
PTR 41-40-05	PhyTip columns (1 mL volume) containing 40 µL of Streptavidin per column	Box of 48	15810	PhyNexus
PTR 41-80-05	PhyTip columns (1 mL volume) containing 80 µL of Streptavidin per column	Box of 48	19890	PhyNexus
PTR 41-16-05	PhyTip columns (1 mL volume) containing 160 µL of Streptavidin per column	Box of 48	29580	PhyNexus
PTR 41-32-05	PhyTip columns (1 mL volume) containing 320 µL of Streptavidin per column	Box of 48	35190	PhyNexus
PTR 91-10-05	PhyTip columns (1 mL volume) containing 10 µL of Streptavidin per column	Box of 96	13464	PhyNexus
PTR 91-20-05	PhyTip columns (1 mL volume) containing 20 µL of Streptavidin per column	Box of 96	15912	PhyNexus
PTR 91-40-05	PhyTip columns (1 mL volume) containing 40 µL of Streptavidin per column	Box of 96	23154	PhyNexus
PTR 91-80-05	PhyTip columns (1 mL volume) containing 80 µL of Streptavidin per column	Box of 96	36618	PhyNexus
PTR 91-16-05	PhyTip columns (1 mL volume) containing 160 µL of Streptavidin per column	Box of 96	52020	PhyNexus
PTR 91-32-05	PhyTip columns (1 mL volume) containing 320 µL of Streptavidin per column	Box of 96	63240	PhyNexus
PTR 41-10-07	PhyTip columns (1 mL volume) containing 10 µL of ProPlus affinty resin per column	Box of 48	9180	PhyNexus
PTR 41-20-07	PhyTip columns (1 mL volume) containing 20 µL of ProPlus affinty resin per column	Box of 48	13056	PhyNexus
PTR 41-40-07	PhyTip columns (1 mL volume) containing 40 µL of ProPlus affinty resin per column	Box of 48	15810	PhyNexus
PTR 41-80-07	PhyTip columns (1 mL volume) containing 80 µL of ProPlus affinty resin per column	Box of 48	19890	PhyNexus
PTR 41-16-07	PhyTip columns (1 mL volume) containing 160 µL of ProPlus affinty resin per column	Box of 48	29580	PhyNexus
PTR 41-32-07	PhyTip columns (1 mL volume) containing 320 µL of ProPlus affinty resin per column	Box of 48	35190	PhyNexus
PTR 91-10-07	PhyTip columns (1 mL volume) containing 10 µL of ProPlus affinty resin per column	Box of 96	13464	PhyNexus
PTR 91-20-07	PhyTip columns (1 mL volume) containing 20 µL of ProPlus affinty resin per column	Box of 96	15912	PhyNexus
PTR 91-40-07	PhyTip columns (1 mL volume) containing 40 µL of ProPlus affinty resin per column	Box of 96	23154	PhyNexus
PTR 91-80-07	PhyTip columns (1 mL volume) containing 80 µL of ProPlus affinty resin per column	Box of 96	36618	PhyNexus
PTR 91-16-07	PhyTip columns (1 mL volume) containing 160 µL of ProPlus affinty resin per column	Box of 96	52020	PhyNexus
PTR 91-32-07	PhyTip columns (1 mL volume) containing 320 µL of ProPlus affinty resin per column	Box of 96	63240	PhyNexus
PTR 41-10-09	PhyTip columns (1 mL volume) containing 10 µL of Normal Phase I resin per column	Box of 48	7140	PhyNexus
PTR 41-20-09	PhyTip columns (1 mL volume) containing 20 µL of Normal Phase I resin per column	Box of 48	10812	PhyNexus
PTR 91-10-09	PhyTip columns (1 mL volume) containing 10 µL of Normal Phase I resin per column	Box of 96	11628	PhyNexus
PTR 91-20-09	PhyTip columns (1 mL volume) containing 20 µL of Normal Phase I resin per column	Box of 96	13362	PhyNexus

PhyNexus 中国代理

发表于2024年11月1日由Amresco中国官网

部分产品报价如下：

货号	品名	规格	目录价	品牌
PTR 41-10-01	PhyTip columns (1 mL volume) containing 10 µL of Protein A affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-01	PhyTip columns (1 mL volume) containing 20 µL of Protein A affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-01	PhyTip columns (1 mL volume) containing 40 µL of Protein A affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-01	PhyTip columns (1 mL volume) containing 80 µL of Protein A affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-01	PhyTip columns (1 mL volume) containing 160 µL of Protein A affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-01	PhyTip columns (1 mL volume) containing 320 µL of Protein A affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-01	PhyTip columns (1 mL volume) containing 10 µL of Protein A affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-01	PhyTip columns (1 mL volume) containing 20 µL of Protein A affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-01	PhyTip columns (1 mL volume) containing 40 µL of Protein A affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-01	PhyTip columns (1 mL volume) containing 80 µL of Protein A affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-01	PhyTip columns (1 mL volume) containing 160 µL of Protein A affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-01	PhyTip columns (1 mL volume) containing 320 µL of Protein A affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-02	PhyTip columns (1 mL volume) containing 10 µL of Protein G affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-02	PhyTip columns (1 mL volume) containing 20 µL of Protein G affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-02	PhyTip columns (1 mL volume) containing 40 µL of Protein G affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-02	PhyTip columns (1 mL volume) containing 80 µL of Protein G affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-02	PhyTip columns (1 mL volume) containing 160 µL of Protein G affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-02	PhyTip columns (1 mL volume) containing 320 µL of Protein G affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-02	PhyTip columns (1 mL volume) containing 10 µL of Protein G affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-02	PhyTip columns (1 mL volume) containing 20 µL of Protein G affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-02	PhyTip columns (1 mL volume) containing 40 µL of Protein G affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-02	PhyTip columns (1 mL volume) containing 80 µL of Protein G affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-02	PhyTip columns (1 mL volume) containing 160 µL of Protein G affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-02	PhyTip columns (1 mL volume) containing 320 µL of Protein G affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-03	PhyTip columns (1 mL volume) containing 10 µL of IMAC affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-03	PhyTip columns (1 mL volume) containing 20 µL of IMAC affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-03	PhyTip columns (1 mL volume) containing 40 µL of IMAC affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-03	PhyTip columns (1 mL volume) containing 80 µL of IMAC affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-03	PhyTip columns (1 mL volume) containing 160 µL of IMAC affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-03	PhyTip columns (1 mL volume) containing 320 µL of IMAC affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-03	PhyTip columns (1 mL volume) containing 10 µL of IMAC affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-03	PhyTip columns (1 mL volume) containing 20 µL of IMAC affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-03	PhyTip columns (1 mL volume) containing 40 µL of IMAC affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-03	PhyTip columns (1 mL volume) containing 80 µL of IMAC affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-03	PhyTip columns (1 mL volume) containing 160 µL of IMAC affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-03	PhyTip columns (1 mL volume) containing 320 µL of IMAC affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-04	PhyTip columns (1 mL volume) containing 10 µL of Glutathione affinity resin per column	Box of 48	7140	PhyNexus
PTR 41-20-04	PhyTip columns (1 mL volume) containing 20 µL of Glutathione affinity resin per column	Box of 48	10812	PhyNexus
PTR 41-40-04	PhyTip columns (1 mL volume) containing 40 µL of Glutathione affinity resin per column	Box of 48	13430	PhyNexus
PTR 41-80-04	PhyTip columns (1 mL volume) containing 80 µL of Glutathione affinity resin per column	Box of 48	16830	PhyNexus
PTR 41-16-04	PhyTip columns (1 mL volume) containing 160 µL of Glutathione affinity resin per column	Box of 48	20910	PhyNexus
PTR 41-32-04	PhyTip columns (1 mL volume) containing 320 µL of Glutathione affinity resin per column	Box of 48	27030	PhyNexus
PTR 91-10-04	PhyTip columns (1 mL volume) containing 10 µL of Glutathione affinity resin per column	Box of 96	11628	PhyNexus
PTR 91-20-04	PhyTip columns (1 mL volume) containing 20 µL of Glutathione affinity resin per column	Box of 96	13362	PhyNexus
PTR 91-40-04	PhyTip columns (1 mL volume) containing 40 µL of Glutathione affinity resin per column	Box of 96	18870	PhyNexus
PTR 91-80-04	PhyTip columns (1 mL volume) containing 80 µL of Glutathione affinity resin per column	Box of 96	29886	PhyNexus
PTR 91-16-04	PhyTip columns (1 mL volume) containing 160 µL of Glutathione affinity resin per column	Box of 96	39780	PhyNexus
PTR 91-32-04	PhyTip columns (1 mL volume) containing 320 µL of Glutathione affinity resin per column	Box of 96	45900	PhyNexus
PTR 41-10-05	PhyTip columns (1 mL volume) containing 10 µL of Streptavidin per column	Box of 48	9180	PhyNexus
PTR 41-20-05	PhyTip columns (1 mL volume) containing 20 µL of Streptavidin per column	Box of 48	13056	PhyNexus
PTR 41-40-05	PhyTip columns (1 mL volume) containing 40 µL of Streptavidin per column	Box of 48	15810	PhyNexus
PTR 41-80-05	PhyTip columns (1 mL volume) containing 80 µL of Streptavidin per column	Box of 48	19890	PhyNexus
PTR 41-16-05	PhyTip columns (1 mL volume) containing 160 µL of Streptavidin per column	Box of 48	29580	PhyNexus
PTR 41-32-05	PhyTip columns (1 mL volume) containing 320 µL of Streptavidin per column	Box of 48	35190	PhyNexus
PTR 91-10-05	PhyTip columns (1 mL volume) containing 10 µL of Streptavidin per column	Box of 96	13464	PhyNexus
PTR 91-20-05	PhyTip columns (1 mL volume) containing 20 µL of Streptavidin per column	Box of 96	15912	PhyNexus
PTR 91-40-05	PhyTip columns (1 mL volume) containing 40 µL of Streptavidin per column	Box of 96	23154	PhyNexus
PTR 91-80-05	PhyTip columns (1 mL volume) containing 80 µL of Streptavidin per column	Box of 96	36618	PhyNexus
PTR 91-16-05	PhyTip columns (1 mL volume) containing 160 µL of Streptavidin per column	Box of 96	52020	PhyNexus
PTR 91-32-05	PhyTip columns (1 mL volume) containing 320 µL of Streptavidin per column	Box of 96	63240	PhyNexus
PTR 41-10-07	PhyTip columns (1 mL volume) containing 10 µL of ProPlus affinty resin per column	Box of 48	9180	PhyNexus
PTR 41-20-07	PhyTip columns (1 mL volume) containing 20 µL of ProPlus affinty resin per column	Box of 48	13056	PhyNexus
PTR 41-40-07	PhyTip columns (1 mL volume) containing 40 µL of ProPlus affinty resin per column	Box of 48	15810	PhyNexus
PTR 41-80-07	PhyTip columns (1 mL volume) containing 80 µL of ProPlus affinty resin per column	Box of 48	19890	PhyNexus
PTR 41-16-07	PhyTip columns (1 mL volume) containing 160 µL of ProPlus affinty resin per column	Box of 48	29580	PhyNexus
PTR 41-32-07	PhyTip columns (1 mL volume) containing 320 µL of ProPlus affinty resin per column	Box of 48	35190	PhyNexus
PTR 91-10-07	PhyTip columns (1 mL volume) containing 10 µL of ProPlus affinty resin per column	Box of 96	13464	PhyNexus
PTR 91-20-07	PhyTip columns (1 mL volume) containing 20 µL of ProPlus affinty resin per column	Box of 96	15912	PhyNexus
PTR 91-40-07	PhyTip columns (1 mL volume) containing 40 µL of ProPlus affinty resin per column	Box of 96	23154	PhyNexus
PTR 91-80-07	PhyTip columns (1 mL volume) containing 80 µL of ProPlus affinty resin per column	Box of 96	36618	PhyNexus
PTR 91-16-07	PhyTip columns (1 mL volume) containing 160 µL of ProPlus affinty resin per column	Box of 96	52020	PhyNexus
PTR 91-32-07	PhyTip columns (1 mL volume) containing 320 µL of ProPlus affinty resin per column	Box of 96	63240	PhyNexus
PTR 41-10-09	PhyTip columns (1 mL volume) containing 10 µL of Normal Phase I resin per column	Box of 48	7140	PhyNexus
PTR 41-20-09	PhyTip columns (1 mL volume) containing 20 µL of Normal Phase I resin per column	Box of 48	10812	PhyNexus
PTR 91-10-09	PhyTip columns (1 mL volume) containing 10 µL of Normal Phase I resin per column	Box of 96	11628	PhyNexus
PTR 91-20-09	PhyTip columns (1 mL volume) containing 20 µL of Normal Phase I resin per column	Box of 96	13362	PhyNexus

PhyNexus 产品报价

发表于2024年10月26日由Amresco中国官网

PhyNexus provides the power of chromatography in a pipette tip column for protein and plasmid purification. We provide life science researchers the solution to high throughput sample processing in drug discovery, screening, structural work etc – any process where very high concentrations of very pure product are desired and where it is important that the biological molecule remains undamaged and active. PhyTip® columns can be used for purification from culture and modifying reactions, expression analysis, immunogenicity screening, CoIP, IP, and ChIP, target immobilization, buffer exchange, glycosylation analysis, process development and biofunctional assays. Through our flexible manufacturing process, PhyNexus can also pack custom resins.

2017产品报价如下：

货号	品名	规格	价格	品牌
PTR 41-10-01	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of Protein A affinity resin per column	1 mL volume	7140	phynexus
PTR 41-20-01	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of Protein A affinity resin per column	1 mL volume	10812	phynexus
PTR 41-40-01	Box of 48 PhyTip columns (1 mL volume) containing 40 µL of Protein A affinity resin per column	1 mL volume	13430	phynexus
PTR 41-80-01	Box of 48 PhyTip columns (1 mL volume) containing 80 µL of Protein A affinity resin per column	1 mL volume	16830	phynexus
PTR 41-16-01	Box of 48 PhyTip columns (1 mL volume) containing 160 µL of Protein A affinity resin per column	1 mL volume	20910	phynexus
PTR 41-32-01	Box of 48 PhyTip columns (1 mL volume) containing 320 µL of Protein A affinity resin per column	1 mL volume	27030	phynexus
PTR 91-10-01	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of Protein A affinity resin per column	1 mL volume	11628	phynexus
PTR 91-20-01	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of Protein A affinity resin per column	1 mL volume	13362	phynexus
PTR 91-40-01	Box of 96 PhyTip columns (1 mL volume) containing 40 µL of Protein A affinity resin per column	1 mL volume	18870	phynexus
PTR 91-80-01	Box of 96 PhyTip columns (1 mL volume) containing 80 µL of Protein A affinity resin per column	1 mL volume	29886	phynexus
PTR 91-16-01	Box of 96 PhyTip columns (1 mL volume) containing 160 µL of Protein A affinity resin per column	1 mL volume	39780	phynexus
PTR 91-32-01	Box of 96 PhyTip columns (1 mL volume) containing 320 µL of Protein A affinity resin per column	1 mL volume	45900	phynexus
PTR 41-10-02	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of Protein G affinity resin per column	1 mL volume	7140	phynexus
PTR 41-20-02	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of Protein G affinity resin per column	1 mL volume	10812	phynexus
PTR 41-40-02	Box of 48 PhyTip columns (1 mL volume) containing 40 µL of Protein G affinity resin per column	1 mL volume	13430	phynexus
PTR 41-80-02	Box of 48 PhyTip columns (1 mL volume) containing 80 µL of Protein G affinity resin per column	1 mL volume	16830	phynexus
PTR 41-16-02	Box of 48 PhyTip columns (1 mL volume) containing 160 µL of Protein G affinity resin per column	1 mL volume	20910	phynexus
PTR 41-32-02	Box of 48 PhyTip columns (1 mL volume) containing 320 µL of Protein G affinity resin per column	1 mL volume	27030	phynexus
PTR 91-10-02	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of Protein G affinity resin per column	1 mL volume	11628	phynexus
PTR 91-20-02	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of Protein G affinity resin per column	1 mL volume	13362	phynexus
PTR 91-40-02	Box of 96 PhyTip columns (1 mL volume) containing 40 µL of Protein G affinity resin per column	1 mL volume	18870	phynexus
PTR 91-80-02	Box of 96 PhyTip columns (1 mL volume) containing 80 µL of Protein G affinity resin per column	1 mL volume	29886	phynexus
PTR 91-16-02	Box of 96 PhyTip columns (1 mL volume) containing 160 µL of Protein G affinity resin per column	1 mL volume	39780	phynexus
PTR 91-32-02	Box of 96 PhyTip columns (1 mL volume) containing 320 µL of Protein G affinity resin per column	1 mL volume	45900	phynexus
PTR 41-10-03	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of IMAC affinity resin per column	1 mL volume	7140	phynexus
PTR 41-20-03	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of IMAC affinity resin per column	1 mL volume	10812	phynexus
PTR 41-40-03	Box of 48 PhyTip columns (1 mL volume) containing 40 µL of IMAC affinity resin per column	1 mL volume	13430	phynexus
PTR 41-80-03	Box of 48 PhyTip columns (1 mL volume) containing 80 µL of IMAC affinity resin per column	1 mL volume	16830	phynexus
PTR 41-16-03	Box of 48 PhyTip columns (1 mL volume) containing 160 µL of IMAC affinity resin per column	1 mL volume	20910	phynexus
PTR 41-32-03	Box of 48 PhyTip columns (1 mL volume) containing 320 µL of IMAC affinity resin per column	1 mL volume	27030	phynexus
PTR 91-10-03	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of IMAC affinity resin per column	1 mL volume	11628	phynexus
PTR 91-20-03	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of IMAC affinity resin per column	1 mL volume	13362	phynexus
PTR 91-40-03	Box of 96 PhyTip columns (1 mL volume) containing 40 µL of IMAC affinity resin per column	1 mL volume	18870	phynexus
PTR 91-80-03	Box of 96 PhyTip columns (1 mL volume) containing 80 µL of IMAC affinity resin per column	1 mL volume	29886	phynexus
PTR 91-16-03	Box of 96 PhyTip columns (1 mL volume) containing 160 µL of IMAC affinity resin per column	1 mL volume	39780	phynexus
PTR 91-32-03	Box of 96 PhyTip columns (1 mL volume) containing 320 µL of IMAC affinity resin per column	1 mL volume	45900	phynexus
PTR 41-10-04	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of Glutathione affinity resin per column	1 mL volume	7140	phynexus
PTR 41-20-04	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of Glutathione affinity resin per column	1 mL volume	10812	phynexus
PTR 41-40-04	Box of 48 PhyTip columns (1 mL volume) containing 40 µL of Glutathione affinity resin per column	1 mL volume	13430	phynexus
PTR 41-80-04	Box of 48 PhyTip columns (1 mL volume) containing 80 µL of Glutathione affinity resin per column	1 mL volume	16830	phynexus
PTR 41-16-04	Box of 48 PhyTip columns (1 mL volume) containing 160 µL of Glutathione affinity resin per column	1 mL volume	20910	phynexus
PTR 41-32-04	Box of 48 PhyTip columns (1 mL volume) containing 320 µL of Glutathione affinity resin per column	1 mL volume	27030	phynexus
PTR 91-10-04	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of Glutathione affinity resin per column	1 mL volume	11628	phynexus
PTR 91-20-04	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of Glutathione affinity resin per column	1 mL volume	13362	phynexus
PTR 91-40-04	Box of 96 PhyTip columns (1 mL volume) containing 40 µL of Glutathione affinity resin per column	1 mL volume	18870	phynexus
PTR 91-80-04	Box of 96 PhyTip columns (1 mL volume) containing 80 µL of Glutathione affinity resin per column	1 mL volume	29886	phynexus
PTR 91-16-04	Box of 96 PhyTip columns (1 mL volume) containing 160 µL of Glutathione affinity resin per column	1 mL volume	39780	phynexus
PTR 91-32-04	Box of 96 PhyTip columns (1 mL volume) containing 320 µL of Glutathione affinity resin per column	1 mL volume	45900	phynexus
PTR 41-10-05	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of Streptavidin per column	1 mL volume	9180	phynexus
PTR 41-20-05	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of Streptavidin per column	1 mL volume	13056	phynexus
PTR 41-40-05	Box of 48 PhyTip columns (1 mL volume) containing 40 µL of Streptavidin per column	1 mL volume	15810	phynexus
PTR 41-80-05	Box of 48 PhyTip columns (1 mL volume) containing 80 µL of Streptavidin per column	1 mL volume	19890	phynexus
PTR 41-16-05	Box of 48 PhyTip columns (1 mL volume) containing 160 µL of Streptavidin per column	1 mL volume	29580	phynexus
PTR 41-32-05	Box of 48 PhyTip columns (1 mL volume) containing 320 µL of Streptavidin per column	1 mL volume	35190	phynexus
PTR 91-10-05	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of Streptavidin per column	1 mL volume	13464	phynexus
PTR 91-20-05	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of Streptavidin per column	1 mL volume	15912	phynexus
PTR 91-40-05	Box of 96 PhyTip columns (1 mL volume) containing 40 µL of Streptavidin per column	1 mL volume	23154	phynexus
PTR 91-80-05	Box of 96 PhyTip columns (1 mL volume) containing 80 µL of Streptavidin per column	1 mL volume	36618	phynexus
PTR 91-16-05	Box of 96 PhyTip columns (1 mL volume) containing 160 µL of Streptavidin per column	1 mL volume	52020	phynexus
PTR 91-32-05	Box of 96 PhyTip columns (1 mL volume) containing 320 µL of Streptavidin per column	1 mL volume	63240	phynexus
PTR 41-10-07	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of ProPlus affinty resin per column	1 mL volume	9180	phynexus
PTR 41-20-07	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of ProPlus affinty resin per column	1 mL volume	13056	phynexus
PTR 41-40-07	Box of 48 PhyTip columns (1 mL volume) containing 40 µL of ProPlus affinty resin per column	1 mL volume	15810	phynexus
PTR 41-80-07	Box of 48 PhyTip columns (1 mL volume) containing 80 µL of ProPlus affinty resin per column	1 mL volume	19890	phynexus
PTR 41-16-07	Box of 48 PhyTip columns (1 mL volume) containing 160 µL of ProPlus affinty resin per column	1 mL volume	29580	phynexus
PTR 41-32-07	Box of 48 PhyTip columns (1 mL volume) containing 320 µL of ProPlus affinty resin per column	1 mL volume	35190	phynexus
PTR 91-10-07	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of ProPlus affinty resin per column	1 mL volume	13464	phynexus
PTR 91-20-07	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of ProPlus affinty resin per column	1 mL volume	15912	phynexus
PTR 91-40-07	Box of 96 PhyTip columns (1 mL volume) containing 40 µL of ProPlus affinty resin per column	1 mL volume	23154	phynexus
PTR 91-80-07	Box of 96 PhyTip columns (1 mL volume) containing 80 µL of ProPlus affinty resin per column	1 mL volume	36618	phynexus
PTR 91-16-07	Box of 96 PhyTip columns (1 mL volume) containing 160 µL of ProPlus affinty resin per column	1 mL volume	52020	phynexus
PTR 91-32-07	Box of 96 PhyTip columns (1 mL volume) containing 320 µL of ProPlus affinty resin per column	1 mL volume	63240	phynexus
PTR 41-10-09	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of Normal Phase I resin per column	1 mL volume	7140	phynexus
PTR 41-20-09	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of Normal Phase I resin per column	1 mL volume	10812	phynexus
PTR 91-10-09	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of Normal Phase I resin per column	1 mL volume	11628	phynexus
PTR 91-20-09	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of Normal Phase I resin per column	1 mL volume	13362	phynexus
PTR 41-10-11	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of Strong Anion resin per column	1 mL volume	7140	phynexus
PTR 41-20-11	Box of 48 PhyTip columns (1 mL volume) containing 20 µL of Strong Anion resin per column	1 mL volume	10812	phynexus
PTR 41-40-11	Box of 48 PhyTip columns (1 mL volume) containing 40 µL of Strong Anion resin per column	1 mL volume	13430	phynexus
PTR 41-80-11	Box of 48 PhyTip columns (1 mL volume) containing 80 µL of Strong Anion resin per column	1 mL volume	16830	phynexus
PTR 41-16-11	Box of 48 PhyTip columns (1 mL volume) containing 160 µL of Strong Anion resin per column	1 mL volume	20910	phynexus
PTR 41-32-11	Box of 48 PhyTip columns (1 mL volume) containing 320 µL of Strong Anion resin per column	1 mL volume	27030	phynexus
PTR 91-10-11	Box of 96 PhyTip columns (1 mL volume) containing 10 µL of Strong Anion resin per column	1 mL volume	11628	phynexus
PTR 91-20-11	Box of 96 PhyTip columns (1 mL volume) containing 20 µL of Strong Anion resin per column	1 mL volume	13362	phynexus
PTR 91-40-11	Box of 96 PhyTip columns (1 mL volume) containing 40 µL of Strong Anion resin per column	1 mL volume	18870	phynexus
PTR 91-80-11	Box of 96 PhyTip columns (1 mL volume) containing 80 µL of Strong Anion resin per column	1 mL volume	29886	phynexus
PTR 91-16-11	Box of 96 PhyTip columns (1 mL volume) containing 160 µL of Strong Anion resin per column	1 mL volume	39780	phynexus
PTR 91-32-11	Box of 96 PhyTip columns (1 mL volume) containing 320 µL of Strong Anion resin per column	1 mL volume	45900	phynexus
PTR 41-10-12	Box of 48 PhyTip columns (1 mL volume) containing 10 µL of Weak Anion resin per column	1 mL volume	7140	phynexus

PhyNexus PTV 92-20-19说明书

发表于2024年10月13日由Amresco中国官网

美国phynexus公司是美国的分子生物学试剂耗材供应商，上海金畔生物科技有限公司为您带来phynexus公司的产品和服务

PhyNexus PTV 92-20-19说明书

C18 Mass Spectrometry PhyTip Columns

PhyNexus开发了一种自动化工艺，使用C18色谱柱和C4色谱柱对蛋白质/多肽进行脱盐或“清理” ，以简化样品制备，进行质谱分析（一次多96个）。当使用手动移液管进行反相纯化时，吸气溶液中的背压会给操作者带来不便，并导致结果不一致。PhyTip色谱柱具有与自动化相辅相成的一致性，易用性和可扩展性，可实现清理。凭借蛋白质结合的高容量和多功能性，PhyNexus C18色谱柱是开发流体MALDI或LC-MS工作流程的适选择。

由于PhyTip移液器吸头柱的床尺寸和容量增加，C18色谱柱与其他色谱柱相比，极性和非极性分子的选择性提高。这种较大的床尺寸将使捕获的平衡推动到增加量的捕获产物。当比较一些的C18的容量时，C18 PhyTip色谱柱的结合量是每毫升树脂蛋白质量的两倍多，同时具有相当的回收率和范围。

C18色谱柱比C4色谱柱更具疏水性，可提供出色的灵敏度和肽的结合。中等90Å和300Å孔均可用于肽和蛋白质片段的差异选择性。在C4和C18树脂之间进行选择时，主要区别在于C4由于其较短的长度和更接近极性二氧化硅基板而比C18稍微更具极性。因此，对于更多极性分子，C4树脂的选择性增加。

这些柱结合了多种蛋白质和多肽大小。结合能力高，较小或更极性的多肽损失较少。在普通的质谱兼容溶剂中回收样品。此外，凝胶过滤柱可用于进行快速且*自动化的96次缓冲液脱盐或缓冲液交换。

使用我们专有的双流色谱 C18提示完整的抗体结合和高终浓度。C18 PhyTip色谱柱兼容大多数8,12和96通道自动化液体处理机器人，包括 Agilent Technologies， Beckman Coulter， Dynamic Devices， Perkin Elmer， Hamilton和 Tecan。

PhyNexus开发了一种自动化工艺，使用C18PhyTip®色谱柱对蛋白质/多肽进行脱盐或“清理”，用于质谱（MS）分析。PhyTip柱通过反相固相萃取（SPE）结合蛋白质/肽，其利用长碳链C18树脂基于其疏水性捕获和分离肽。

PhyTip色谱柱由C18树脂组成，通过两个薄的惰性玻璃料屏幕保留在移液器吸头上。根据下游分析，PhyTip色谱柱可以在5μL床中使用90Å或300Å孔隙树脂制成，并且可以处理高达200μL的溶液。PhyTip色谱柱通过双流色谱处理肽，这是一种由控制体积和流速的液体处理器执行的来回流动的系统过程。

因此，该方法可用于清除中小规模的肽样品（每次多96个），用于基质辅助激光解吸电离（MALDI）或电喷雾电离（ESI）质谱。自动化过程的一部分。

PhyNexus MEA个人净化系统的样品处理

1.样品制备成0.1％三氟y酸（TFA）浓度

2.用100μL100％乙腈（ACN）润湿4个循环

3.用100μL0.1％TFA平衡1个循环

4.用185μL样品捕获4个循环

5.用100μL0.1％TFA洗涤1个循环

6.用100μL70％ACN + 0.1％TFA洗脱4个循环

每个循环包括一次抽吸和一次排出，流速为1 mL / min。每个洗脱抽吸/排出步骤以4个增量进行，暂停5秒，而所有其他抽吸/排出步骤以8个增量进行，暂停30秒。该处理旨在模拟手动移液，同时补偿背压。密封洗脱板以防止挥发性洗脱缓冲液在处理期间蒸发，并且将溶液真空脱气以进一步确保空气不进入柱。

质谱PhyTip色谱柱可用于这些机器人系统。

树脂	机器人系统	提示量	树脂量	数量/箱	零件号	描述
C18300Å	Agilent Velocity 11 Bravo和V-Prep	200	20	96	PTV 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Beckman FX＆NX 96孔	200	5	96	PTB 92-05-19	每盒含有5μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Beckman FX＆NX 96孔	200	20	96	PTB 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	5	96	PTZ 92-05-19	每盒含有5μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	20	96	PTZ 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	动态设备Oasis＆Lynx	200	5	96	PTD 92-05-19	每盒含有5μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	动态设备Oasis＆Lynx	1000	5	96	PTD 91-10-19	每盒含有10μLC18反相（300Å孔）树脂的96盒PhyTip柱（1 mL体积）
C18300Å	动态设备Oasis＆Lynx	1000	10	96	PTX 91-10-19	每盒含有10μLC18反相（300Å孔）树脂的96盒PhyTip柱（1 mL体积）
C18300Å	动态设备Oasis＆Lynx	200	20	96	PTD 92-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（200μL体积）
C18300Å	动态设备Oasis＆Lynx	1000	20	96	PTD 91-20-19	每盒含有20μLC18反相（300Å孔）树脂的96个PhyTip色谱柱（1 mL体积）

PhyNexus PTV 92-20-01说明书

发表于2024年10月13日由Amresco中国官网

美国phynexus公司是美国的分子生物学试剂耗材供应商，上海金畔生物科技有限公司为您带来phynexus公司的产品和服务

PhyNexus PTV 92-20-01说明书

PhyNexus开发了一种*的技术，用于工程蛋白和抗体的微量纯化，使研究人员能够定期纯化和富集高达1 mL的样品。PhyTip™色谱柱的*设计使洗脱体积低至10μL，从而产生高达50 x的富集因子，纯化蛋白浓度高达5 mg / ml。

纯化和富集的过程是简单的三步技术，其中首先捕获感兴趣的蛋白质，然后纯化并终富集。整个过程可能需要不到15分钟的时间来生成高浓度的全功能蛋白质，可供进一步分析。

IgG回收率为70-90％
获得高度浓缩的纯化蛋白质
蛋白质A PhyTip柱显示从不同水平的背景蛋白质中提取具有非常高纯度的IgG蛋白质

蛋白质A PhyTip柱显示出功能性抗体（IgG）的高结合亲和力和高容量亲和纯化。

蛋白A柱采用我们专有的双流色谱，可提供完整的抗体结合和高终浓度。PhyTip色谱柱兼容大多数8,12和96通道自动化液体处理机器人，包括 Agilent Technologies， Beckman Coulter， Dynamic Devices， Perkin Elmer， Hamilton和 Tecan。

具有蛋白A的PhyTip色谱柱已针对特定PhyNexus试剂和仪器流速/体积进行了优化，如下所示。使用PhyNexus纯化系统收集该信息。带有蛋白A的所有PhyTip色谱柱都配有推荐的PhyTip缓冲液，包括：捕获缓冲液 – 用于需要添加额外缓冲液以补充样品体积并确保捕获正确pH值的情况。洗涤缓冲液I – 磷酸盐缓冲溶液pH 7.4洗涤缓冲液II – 盐水溶液。注意：没有缓冲能力以确保有效洗脱富集缓冲液 – 用于终洗脱步骤 – 磷酸盐缓冲溶液pH 2.5中和缓冲液。 – Tris缓冲溶液pH 9.0注意：富集缓冲液以4 mL pH 2.5磷酸盐缓冲溶液的形式提供，

含有蛋白A树脂的200多个PhyTip色谱柱：

对于含有1mg BSA的5μgIgG2a（抗FITC MAb。）的200μL样品，使用下面显示的条件处理，在终样品体积中回收大于40％的原始IgG质量。此外，通过用考马斯检测的SDS-PAGE测定，回收的IgG纯度超过95％。

捕获：通过树脂床以250μL/分钟的流速通过树脂床捕获200μL样品。

纯化：200μLPhyNexus蛋白A洗涤缓冲液I，以500μL/ min的流速通过树脂床两个循环，然后用洗涤缓冲液II进行第二次洗涤，在树脂床上通过流动两个循环速率为500μL/ min。

富集：用10μLPhyNexusProtein A Enrichment Buffer将蛋白质洗脱到溶液中，以500μL/ min的流速通过树脂床5个循环。用3μLPhyNexus蛋白A中和缓冲液中和。

出厂时含有的蛋白G富集缓冲液含有：111mM NaH2PO4,140mM NaCl，14.8mM H3PO4，pH3.0

含有蛋白A树脂的1000多种PhyTip色谱柱：

对于含有5μgBSA的10μgIgG2a（抗FITC MAb。）的500μL样品，使用下面显示的条件处理，在终样品体积中回收大于40％的原始IgG质量。此外，通过用考马斯检测的SDS-PAGE测定，回收的IgG纯度超过95％。

捕获：通过树脂床以250μL/分钟的流速通过四个循环捕获500μL样品。

纯化：1000μLPhyNexusProtein G洗涤缓冲液I，以500μL/ min的流速通过树脂床两个循环，然后用洗涤缓冲液II进行第二次洗涤，以500的流速通过树脂床两个循环。 μL/分钟。

富集：用15mL PhyNexus蛋白A富集缓冲液将蛋白质洗脱到溶液中，以500μL/ min的流速通过树脂床5个循环。用5μLPhyNexus蛋白A中和缓冲液中和。

蛋白质APhyTip®色谱柱的性能特征和分析程序

介绍

PhyNexusPhyTip®色谱柱是创新的纯化工具，可从根本上简化各种来源的蛋白质的捕获，纯化和富集。这些净化工具成功的关键是设计保留亲和树脂床的机制，具有小的死体积和大的捕获潜力。一组PhyTipcolumns包含蛋白A，以在特定适条件下特异性结合来自不同来源的抗体（IgG），从而允许去除核酸和其他污染物。在快速洗涤步骤后，用常用的低pH缓冲液容易地洗脱纯化的抗体。该技术允许特别高产率的IgG，这取决于各种条件，并且在非常高浓度的载体蛋白如BSA的存在下提供高度选择性的纯化。PhyTip色谱柱具有*的结合能力，可以结合超过100μg的IgG，并且可以有效回收少至200 ng的IgG – 代表近三个数量级的范围。PhyTip色谱柱非常适用于从杂交瘤上清液中筛选抗体或用于高通量提取和纯化亲和标记蛋白。

绩效和分析程序

IgG以高亲和力和特异性结合蛋白A. 这种相互作用的强度和选择性使蛋白A能够有效地从复杂的蛋白质混合物中纯化IgG。为了检测PhyTip柱与蛋白A树脂的性能，测量了使用1000+蛋白A PhyTip柱的纯化IgG（抗FITC-MAb）的回收百分比。

材料和方法

样品：10μgmFITC-MAb（IgG2a亚类）在0.5ml
含有5mg BSA的PBS中，或
2.含有10％FBS（胎牛血清）的DMEM ，或含有5mg BSA的3.SFM
（无血清培养基）
样品处理：使用以下方案在半自动ME 1000平台上处理所有样品：

（1）捕获：通过将样品通过树脂床进行2个输入/输出循环（体积编程为0.6ml，0.25ml / min）来捕获特定蛋白质。
（2）纯化：通过用0.5mL PBS（洗涤缓冲液I）进行1个输入/输出循环（体积编程为0.6ml，0.5ml / min）洗涤结合的蛋白质/亲和树脂，然后进行1个输入/输出循环，去除未结合的蛋白质（体积为0.6ml，0.5ml / min），用0.5mL盐水溶液（洗涤缓冲液II）。
（3）富集：用15μLpH3.0洗脱缓冲液以4次进/出循环（体积编程为0.1-0.15ml，1ml / min）洗脱IgG2a。洗脱后，加入5μL中和缓冲液。注意，由PhyNexus提供的蛋白A和蛋白G PhyTip柱的当前洗脱缓冲液具有2.5的pH，并且当前的中和缓冲液的pH为9.0。

定量程序

（1）为了制备还原的IgG2a，使15μl终洗脱体积与5μl新制备的TCEP（15μl10mg/ ml TCEP在水中反应，终体积为30μl）反应。

（2）使用HP 1050 HPLC系统将20μl还原的IgG2a注入无孔聚苯乙烯二乙烯基苯反相（C-18）柱中。溶剂A（水中0.1％TFA）和溶剂B（ACN中0.1％TFA）之间的梯度为25％至75％，使用5分钟。检测：214和280nm的UV。

（3）T wo主要IgG2a峰在3.18和3.34分钟左右洗脱。在每种情况下，将这两个峰下面积从（3.3-13.5）min积分，并在214nm处记录相应的峰面积。
（4）将相同反应条件下的TCEP处理的IgG2a标准品加载到柱中，并用作恢复计算的输入或标准。

IgG2a恢复的结果

在DMEM + 10％FBS中的回收率= 77％SFM中的回收率+ 5mg BSA = 64％。

已知载体蛋白如白蛋白诱导免疫应答并有助于产生良好的抗体，因此当产生抗体时它通常以高量存在。因此，任何纯化和富集程序必须能够耐受高浓度的白蛋白。此外，上面显示的结果似乎表明高背景蛋白浓度（如BSA）的存在可能对回收率有益。

在载体蛋白存在下IgG2a回收率的再现性

为了验证载体蛋白对大IgG2a回收率的作用，并证明重现性，从多个样品（n = 4）中从PBS（以及含有5mg BSA的PBS）中纯化IgG2a。结果表明，在500倍过量的BSA浓度存在下，纯化的IgG2a的回收率提高（至少20％或更多），并且该方法也是高度可重复的。

PBS中的回收率= 42.25％（SD = 4.66％）PBS中的回收率+ 5mg BSA = 66.5％（SD = 7.5％）

选择性表现和分析程序

为了表征和验证蛋白A-IgG亲和相互作用的选择性，将5mg BSA作为载体蛋白添加至0.5μg样品体积（333倍过量）中的15μgIgG。使用Protein A PhyTip柱处理样品。

材料和方法

样品：在0.5ml含有5mg BSA（10mg / ml或1％w / v BSA）的PBS或PBS中的15μgmFITC-MAb（IgG2a亚类）。样品处理：如上文“恢复性能和分析程序”中所述。

IgG定量和纯度分析程序：

（1）将15μl终洗脱体积分成两部分：13μl与新制备的13μl10mg / ml TCEP（终体积=26μl和[TCEP] = 17.5 mM）在室温下反应~16在每个实验中将2μl加载到凝胶上。

（2）使用HP 1050 HPLC系统将20μl以上26μl还原的IgG2a注入无孔聚苯乙烯二乙烯基苯反相（C-18）柱中。溶剂A（水中0.1％TFA）和溶剂B（ACN中0.1％TFA）之间的梯度为25％至75％，使用5分钟。检测：214和280nm的UV。

（3）两个主要的IgG2a峰在3.17和3.3分钟左右洗脱。在这两个峰下面积在每种情况下从（3.13-3.5）min积分，并且在214nm处记录相应的mAU。
（4）将在相同反应条件下经TCEP处理的IgG2a标准品（注射量为1.08,2.6,4.3,6.48和8.64μgFITC-M Ab）装入柱中并用作恢复计算的标准曲线。

PhyNexus建议使用含有IgG的PhyTip入门试剂盒作为标准，以验证所有应用中PhyTip色谱柱的使用。

用于纯化和富集的样品必须清澈且无颗粒物质。在与PhyTip色谱柱一起使用之前，强烈建议离心样品并仅使用澄清的上清液。

运输和存储

每包PhyTip色谱柱均按照高标准生产和质量控制，并在保留盒中运输，以保持每个PhyTip色谱柱中特定亲和树脂的完整性。本产品在环境温度下运输，但收到后应存放在4到8℃的标准实验室冰箱中。

不要冷冻或储存冷冻。
不使用时，请将盒盖关闭并密封，存放在冰箱中。
在干燥环境中长时间储存，不要让亲和树脂干燥。

当从PhyNexus运输时，含有蛋白A的PhyTip柱存储在甘油中。

蛋白质A PhyTip色谱柱可用于这些机器人系统

机器人系统

Agilent Velocity 11 Bravo和V-Prep

Beckman FX＆NX 96孔

Caliper SciClone和Zephyr 96通道（现为Perkin Elmer）

动态设备Oasis＆Lynx

Hamilton STAR和Nimbus

Perkin Elmer Janus Varispan 4或8孔

Perkin Elmer Packard MiniTrack和Janus MDT 96频道

PhyNexus MEA或ME

Tecan Freedom Evo LiHa＆Tecan Te-MO

Tecan Freedom Evo MCA 96孔

树脂	机器人系统	提示量	树脂量	数量/箱	零件号	描述
蛋白质A.	Agilent Velocity 11 Bravo和V-Prep	200	20	96	PTV 92-20-01	一箱96个PhyTip柱（200μL体积）每柱20μL蛋白A亲和树脂
蛋白质A.	Beckman FX＆NX 96孔	200	五	96	PTB 92-05-01	一箱96个PhyTip柱（200μL体积）每柱5μL蛋白A亲和树脂
蛋白质A.	Beckman FX＆NX 96孔	200	20	96	PTB 92-20-01	一箱96个PhyTip柱（200μL体积）每柱20μL蛋白A亲和树脂
蛋白质A.	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	五	96	PTZ 92-05-01	一箱96个PhyTip柱（200μL体积）每柱5μL蛋白A亲和树脂
蛋白质A.	Caliper SciClone＆Zephyr 96 channel_now Perkin Elmer	200	20	96	PTZ 92-20-01	一箱96个PhyTip柱（200μL体积）每柱20μL蛋白A亲和树脂
蛋白质A.	动态设备Lynx	1000	10	96	PTX 91-10-01	每盒含有10μL蛋白A亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质A.	动态设备Lynx	1000	160	96	PTX 91-16-01	每盒含有160μL蛋白A亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质A.	动态设备Lynx	1000	20	96	PTX 91-20-01	每盒含有20μL蛋白A亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质A.	动态设备Lynx	1000	40	96	PTX 91-40-01	每盒含有40μL蛋白A亲和树脂的96个PhyTip柱（1 mL体积）
蛋白质A.	动态设备Lynx	1000	80	96	PTX 91-80-01	每盒含有80μL蛋白A亲和树脂的96个PhyTip柱（1 mL体积）

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