标签归档：plvx

pLVX-IRES-ZsGreen1载体说明书

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 pLVX-IRES-ZsGreen1载体

型号 载体名称 出品公司 载体用途
VMC0380 pLVX-IRES-ZsGreen1 Clontech 慢病毒载体

Description

pLVX-IRES-ZsGreen1 is an HIV-1-based, lentiviral expression vector that allows the simultaneous 

expression of your protein of interest and ZsGreen1 in virtually any mammalian cell type, 

including primary cells. ZsGreen1 is a human codon-optimized variant of the reef coral 

Zoanthus sp. green fluorescent protein, ZsGreen. The vector expresses the two proteins from 

a bicistronic mRNA transcript, allowing ZsGreen1 to be used as an indicator of transduction 

efficiency and a marker for selection by flow cytometry.

Expression of the bicistronic transcript is driven by the constitutively active human 

cytomegalovirus immediate early promoter （P

CMV IE） located just upstream of the MCS. An 

encephalomyocarditis virus （EMCV） internal ribosome entry site （IRES）, positioned between 

the MCS and ZsGreen1, facilitates cap-independent translation of ZsGreen1 from an internal 

start site at the IRES/ZsGreen1 junction （1）

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pLVX-AcGFP1-N1载体说明书

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 pLVX-AcGFP1-N1载体

型号 载体名称 出品公司 载体用途
VMC0381 pLVX-AcGFP1-N1 Clontech 慢病毒载体

Description

pLVX-AcGFP1-N1 is an HIV-1-based, lentiviral expression vector that allows you to express 

your gene of interest fused to AcGFP1, a green fl uorescent protein derived from Aequorea 

coerulescens. Genes cloned into the multiple cloning site （MCS）, located upstream of the 

AcGFP1 coding sequence, are expressed as N-terminal fusions of the AcGFP1 protein. 

Expression of the fusion protein is driven by the constitutively active human cytomegalovirus

immediate early promoter （P

CMV IE） located just upstream of the MCS. Lentiviral particles 

derived from the vector allow the expression of AcGFP1 fusion proteins in virtually any cell 

type, including primary cells. The unmodifi ed vector expresses AcGFP1, and may be used to 

produce marker virus to optimize infection protocols.

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pLVX-DsRed-Monomer-N1载体

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 pLVX-DsRed-Monomer-N1载体

型号 载体名称 出品公司 载体用途
VMC0379 pLVX-DsRed-Monomer-N1 Clontech 慢病毒载体

Description

pLVX-DsRed-Monomer-N1 is an HIV-1-based, lentiviral expression vector that allows you 

to express your gene of interest fused to DsRed-Monomer, a monomeric mutant of the 

Discosoma sp. red fluorescent protein. Genes cloned into the multiple cloning site （MCS）, 

located upstream of the DsRed-Monomer coding sequence, are expressed as N-terminal 

fusions of the DsRed-Monomer protein. Expression of the fusion protein is driven by the 

constitutively active human cytomegalovirus immediate early promoter （P

CMV IE） located 

just upstream of the MCS. Lentiviral particles derived from the vector allow the expression 

of DsRed-Monomer fusion proteins in virtually any cell type, including primary cells. The 

unmodified vector expresses DsRed-Monomer, and may be used to produce marker virus to 

optimize infection protocols.

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pLVX-shRNA2载体说明书

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 pLVX-shRNA2载体

型号 载体名称 出品公司 载体用途
VMC0539 pLVX-shRNA2 Clontech 慢病毒载体
载体描述: 

pLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA （shRNA） for RNA interference （RNAi） studies. Expression of your shRNA is driven by the RNA Pol III-dependent, human U6 promoter （PU6）, located just upstream of the MCS. pLVX-shRNA2 can be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived from the vector allow the expression of shRNAs in virtually any cell type, including primary cells.

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pLVX-shRNA1载体说明书

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 pLVX-shRNA1载体说明书

型号 载体名称 出品公司 载体用途
VMC0538 pLVX-shRNA1 Clontech 慢病毒载体
载体描述: 

The pLVX-shRNA1 Vector is a lentiviral expression vector that can be used to generate high titers of lentivirus for transducing dividing or nondividing mammalian cells. Transduced target cells will express a user-generated shRNA from the U6 promoter for the purpose of RNAi-mediated gene silencing. pLVX-shRNA1 Vector can be packaged into high-titer, replication-incompetent, VSV-G-pseudotyped lentivirus upon transfection into 293T cells, using the Lenti-X™ HTX Packaging System. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.

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