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pMAL-c6T Vector
This product is a replacement for NEB #N8108, pMAL™-c5X Vector
The pMAL-c6T Vector is designed to produce maltose-binding protein (MBP) fusions in the cytoplasm. The MBP has been engineered for tighter binding to amylose resin. The vector expresses an N-terminal his-tagged malE gene followed by a multiple cloning site containing a TEV protease recognition sequence, allowing MBP to be cleaved from the protein of interest after purification.
- View sequence details
- pMAL-c6T is the recommended vector for use with the NEBExpress® MBP Fusion and Purification System (NEB #E8201)
- Fusion to MBP significantly enhances the solubility and proper folding of proteins expressed in E. coli (1)
- Vector includes His-tag and TEV Protease recognition site, allowing the protein of interest to be cleaved from MBP using TEV Protease (NEB #P8112)
- Two-step purification: amylose resin elution followed by TEV Protease cleavage and Ni resin isolation results in a highly-pure, tag-free target protein
- MBP has been engineered for tighter binding to amylose resin
- Ampicillin resistant
Anatrace Amphipol PMAL-C8 货号：P5008 CAS号：869856-84-8
美国Anatrace是全球公认的膜蛋白研究产品专业供应商，为膜蛋白提取与纯化提供高纯度生化试剂，包括各种表面活性剂、去垢剂、磷脂、实验室通用试剂及各种研究用工具书等。
膜蛋白研究常用高纯度去垢剂（Detergent）
在蛋白纯化过程中需要选择合适的去垢剂使膜蛋白本身保持空间构象和活性。非离子型去垢剂较为温和，不易导致蛋白质变性，应用十分广泛；离子型去垢剂对于膜蛋白溶解非常有效；两性离子去垢剂，通常用于膜蛋白结晶，并结合了离子型和非离子型去垢剂的优点。
自20世纪80年代末以来，Anatrace一直是高纯度和高标准去垢剂生产领导者。自推出第一款去垢剂十二烷基麦芽糖（DDM）以来，其产品组合成长迅速，可提供超过50种独特的去垢剂产品。
品名：Amphipol PMAL-C8
货号：P5008
CAS号：869856-84-8
|
产品名称 |
描述 |
CAS号 |
货号 |
|
GDN |
毛地黄皂苷替代物 |
1402423-29-3 |
GDN101 |
|
DDM |
正十二烷基–β-D-吡喃麦芽糖苷 |
69227-93-6 |
D310 |
|
DM |
1-O-癸基–β-D-麦芽糖苷 |
82494-09-5 |
D322 |
|
Amphipol A8-35 |
两亲性表面活性剂 |
326856-53-5 |
A835 |
|
Amphipol PMAL-C8 |
PMAL是两性离子去垢剂 |
869856-84-8 |
P5008 |
|
LMNG |
月桂基麦芽糖新戊二醇 |
1257852-96-2 |
NG310 |
|
OG |
辛基–β-D-吡喃葡萄糖苷 |
29836-26-8 |
O311 |
|
LDAO |
月桂基二甲基氧化胺 |
1643-20-5 |
D360 |
|
LAPAO |
月桂酰胺丙基胺氧化物 |
61792-31-2 |
L360S |
|
CHAPS |
两性离子去垢剂 |
75621-03-3 |
C316 |
|
UDM |
十一烷基-BETA-D-麦芽糖苷 |
253678-67-0 |
U300 |
|
CHS |
胆甾醇琥珀酸酯 |
102601-49-0 |
CH210 |
|
CYMAL-6 |
6-环己基己基4-O-ALPHA-D-吡喃葡萄糖基-BETA-D-吡喃葡萄糖苷 |
228579-27-9 |
C326 |
|
DMNG |
癸基麦芽糖新戊二醇 |
1257852-99-5 |
NG322 |
|
OMNG |
辛基葡萄糖新戊二醇 |
1257853-32-9 |
NG311 |
|
L-(+)-Selenomethionine |
L-硒代蛋氨酸 |
3211-76-5 |
S2000 |
|
FLUORINATED FC-8 |
氟化 Fos-Choline-8 |
313997-22-7 |
F300F |
|
FLUORINATED OM |
氟化辛基麦芽糖苷 |
118680-70-9 |
O310F |
|
NAPol |
非离子型两亲性去垢剂 |
NAP |
|
|
DIBMA |
无去垢剂聚合物体系，模拟天然磷脂双分子层 |
BMA101 |
|
|
10:1 DDM/CHS |
配方成分：十二烷基麦芽苷（D310）和胆甾醇半胱氨酸（CH210） |
D310-CH210 |
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