Polyscience rhodamine B


Polyscience rhodamine B

简要描述:甲基丙烯酰氧,乙基硫代氨基甲酰,罗丹明B

详细介绍

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Methacryloxyethyl thiocarbamoyl rhodamine B

N-[9-(2-carboxy-x-methacryloxy-ethylthiocarbamoylphenyl)-6-diethylamino-3H-xanthen-3-ylidene]-N-ethyl-ethanaminium chloride; PolyFluor® 570

Fluorescent monomer. 

Ex. max: 548nm    Em. max: 570nm

分子量: 683.24
外观: 紫色晶体
操作: 手套,通风柜
存储: 避免光照,常温保存。

PEI与Polyscience 24765-1效果对比

 

 上海金畔生物自产BioHuB PEI与Polyscience 24765-1效果对比

 

实验结果反馈表

单位

**大学模式生物研究所

实验组名称

***实验室

实验室研究方向

神经发育及再生

试用人

**

 

转染细胞种类

293T

现用转染试剂品牌

Polyscience 24765-1

 

转染选用质粒

 

pLKO.1

 

现用转染试剂价格

 

1g/****

转染后观察时间

24/48hr

现用转染试剂规格及

平均可用时长

1g  2-3

 

相同转染条件下, BioHuB PEI与现用转染试剂效果对比

 

BioHuB PEI转染效率:70-90%

现用转染试剂转染效率:70-90%

试用结果图片展示:

 

具体试用效果及建议:

PEIMAX-40k 相比上代PEI-25k 整体转染效率增强,且在含血清培养基条件下仍可有效转染,且转染比例较高。对常用易转染细胞系实验,相比lipo系列,有较高的性价比。在一定条件下,适当增加transfer DNA量,可明显提高细胞转染效率及蛋白表达,且细胞活力正常。值得注意的是,过量PEIMAX 转染可能会产生细胞毒性,需对实验细胞进行预测试。

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Polyscience

简要描述:

我们的所有产品-冷冻循环器、加热循环器、冷冻机、循环冷却器、水浴和定制设备-都是由美国训练有素的工程团队设计和开发的,符合我们自己严格的标准和ISO 9001质量要求。

我们的所有产品-冷冻循环器、加热循环器、冷冻机、循环冷却器、水浴和定制设备-都是由美国训练有素的工程团队设计和开发的,符合我们自己严格的标准和ISO 9001质量要求。五十年后,我们不再是一家初创企业。但是,对创新的热情,对质量的承诺,以及用户关注继续推动着我们设计和制造优秀产品的热情。让我们成为你液体温度控制的搭档。


Back in 1963, we were a startup. And like many global companies, we started out small but kept thinking big. Along the way, we pioneered innovations ranging from the first zero-switching circulators to the first refrigeration systems for DNA amplification to an award-winning line of culinary products. Over one million temperature control solutions have been sold under our brand name or as private label for large, well-respected manufacturers and distributors. Our dedication to our customers, and their needs, has helped us become a global company that spans six continents and most major cities.


All of our products—Refrigerated Circulators, Heated Circulators, Chillers, Recirculating Coolers, Water Baths, and Custom Equipment—are designed and developed by a highly trained engineering team in the U.S.A. to our own rigid standards as well as ISO 9001 quality requirements. After five decades, we're no longer a startup. But the passion for innovation, commitment to quality, and unmatched user-focus continue to drive our enthusiasm for designing and manufacturing superior products. Making us your perfect partner for liquid temperature control.


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