Visual Protein磷蛋白富集试剂盒


Visual Protein磷蛋白富集试剂盒PhosPROTM 磷蛋白富集试剂盒

 

PhosPROTM 磷蛋白富集试剂盒依据 IMAC 原理改良而成,可自各种生物样本分离大量磷酸化蛋白质,包含动物、植物与微生物。PhosPROTM 磷蛋白富集试剂盒可提高磷酸化蛋白质分离量,大量的磷酸化蛋白质可应用于后续的蛋白质印迹法实验。

产品特点:

 

增加产品运用灵活性

提供多样化的规格可供选择适合不同需求的样品族群

PhosPROTM 磷蛋白富集试剂盒有效分離且富集四種细胞株的磷酸化蛋白

HepaG2: Human hepatocellular liver carcinoma cell line NS-1: Mouse myeloma cell line NS-1

RMC: Rat mesangial cell

SP2: Mouse myeloma cell line SP2 F: Flow through

E: Eluent

 

VisPROTM 5 Minutes Protein Stain Kit (VisPROTM 蛋白质染色试剂盒) and Pro-Q Diamond stain (Pro-Q, Invitrogen) were used for developing total proteins or phosphoproteins.

 VisualProtein PP03-2C说明书

货号:PP03-2C

品名:PhosPROTM Phosphoprotein Enrichment Kit

规格:1 kit  2 pre-packed column 2mL Lysis buffer 250mL System buffer

           100mL Elution buffer

    100mL Stringent buffer stock

 

 

货号:PP03-6C

品名:PhosPROTM Phosphoprotein Enrichment Kit

规格:1 kit    6 pre-packed column 2mL Lysis buffer 250mL System buffer

      100mL Elution buffer

              100mL Stringent buffer stock

 

货号:PP03-5E

  品名:PhosPROTM Phosphoprotein Enrichment Kit

规格:1 kit     

3mL resin

2mL Lysis buffer

100mL Elution buffer

100mL Stringent buffer stock

250mL System buffer

 

Visual Protein化学发光检测试剂

Visual Protein根据实验解决问题的原则,我们提供高质量的产品:(1)蛋白质印迹法;(2)蛋白质纯化;(3)抗体生产;(4)蛋白质组学;(5)缓冲液。

 

 

LumiFlashTM Infinity 强效型化学发光检测试剂

LumiFlashTM Infinity 强效型化学发光检测试剂为 Lumi-

FlashTM 系列高阶产品,灵敏度高,侦测灵敏度为 femto- gram (10-15 g) 等级,适合用于侦测一般蛋白质印迹法不易侦测到的极度微量蛋白质。有些高灵敏度化学发光试剂,在进行蛋白质印迹法时若接触到目标蛋白质量很多,会造成发光试剂无法发光,并在转印膜上留下褐色的阴影,此现象称为burn,其原因为酵素量太多,造成发光试剂作用后于目标蛋白质位置产生沉淀现象而无法发光。LumiFlashTM强效型化学发光检测试剂经过实验测试,可避免 Burn 现象发生,能忠实呈现侦测到目标蛋白质的讯息。

产品特点:

灵敏度达 femtogram 等级

发光时间长,可配合长曝光实验可减少抗体用量

更高的灵敏度

LumiFlashTM Infinity 强效型化学发光检测试剂侦测灵敏度试验

Hela cell lysate with 1/2 serial dilution from 20 µg was separated by 12.5% SDS-PAGE and probed by anti-AMPKα1. All results were exposed to x-ray film for 5 minutes.

 

订购信息:

货号:LF16-100

  品名:LumiFlashTM Infinity Chemiluminescent Substrate

    规格:1 kit   50mL soultion A + 50mL solution B

 

货号:LF16-500

品名:LumiFlashTM Infinity Chemiluminescent Substrate

规格:1 kit 250mL soultion A + 250mL solution B

 

 

 

Visual Protein BP01-1L说明书

VisualProtein于2005年在中国台湾成立,我们致力于通过提供研究用的服务和创新产品来促进生物科学的发展。2015年,视觉蛋白与能源生物医学有限公司联手,在能源生物医学的资源和支持下,视觉蛋白将继续提供有效的产品,确保我们的客户获得可靠的结果。我们对未来的愿景是为先进的研究和开发提供更好的解决方案。

根据实验解决问题的原则,我们提供高质量的产品:(1)蛋白质印迹法;(2)蛋白质纯化;(3)抗体生产;(4)蛋白质组学;(5)缓冲液。

Visual Protein BP01-1L说明书

BlockPROTM 封闭缓冲液

BlockPROTM 封闭缓冲液成份单纯,能有效阻断实验中可能产生非专一性结合的位置,不会遮盖抗原,提高抗原与抗体结合之专一性。

 

产品特点

优异遮蔽:可降低背景噪声,使目标蛋白更清晰无 biotin:适用于 biotin-avidin 统

无磷酸化蛋白:适用于侦测磷酸化蛋白

 

    实验数据:

 

 

有效遮蔽

使用原子力显微镜观察封闭效果,BlockPROTM 封闭缓冲液能有效遮蔽非抗原区域

30 µg cell lysate (HepaG2) separated by 12.5% SDS-PAGE and blocking by skim milk or Block- PRO™ Blocking Buffer. Membranes detected by SEM. The SEM result showed that BlockPRO™ Blocking Buffer only blocking on excess binding site and reveal the position of antigen and therefore will not reduce the signal intensity.

 

更佳的目标讯号

在相同实验条件下透过化学发光系统侦测, BlockPROTM 封闭缓冲液表现更优异

Loading 30 µg cell lysate (HepaG2) and detect with anti-AMPK (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP 1:10,000.

Membrane: Hyond™ P. Detection : Hyperfilm™ ECL. All results were exposed for 30 seconds and capture by X-ray film.

 

订购信息:

货号:BP01-1L

 

品名:BlockPROTM Blocking Buffer

 

规格:1 kit   500mL x 2

 

 

 

Phosphosolutions公司Anti-ABCA4 (Rim Protein)产品代理


Phosphosolutions公司Anti-ABCA4 (Rim Protein)产品代理

简要描述:公司概况

背景
基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。
蛋白质体学项目:Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。
PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。

our focus 专业特色
PhosphoSolutions公司专注于蛋白质组学中的一个(10-20

详细介绍

产品咨询

 Antibodies 抗体

特异性磷抗体:Detection and quantitation of changes in the state of phosphorylation of specific proteins is of great utility in the quest to establish the function of a given protein and the consequences of its reversible phosphorylation. Two methods commonly used to measure protein phosphorylation and dephosphorylation in cell preparations employ prelabeling with 32Pi or back phosphorylation. These methods continue to be very effective and have advantages for many test systems, but they do have several practical and theoretical limitations (Nestler and Greengard, 1984). Based in large part on the successful use of short synthetic peptides to produce epitope-targeted antibodies (Lerner, 1982;Sutcliffe et al., 1983), an immunochemical approach became an attractive alternative for detecting changes in the state of phosphorylation of specific proteins at a specific site. The use of phosphorylation state-specific antibodies takes advantage of the sensitivity and selectivity afforded by immunochemical methodology, combined with relatively simple preparation and potentially broad applications.

The first report of phosphorylation-dependent antibodies appeared in 1981, when polyclonal antibodies that could detect phosphotyrosine-containing proteins were produced by immunization with benzyl phosphonate conjugated to keyhole limpet hemocyanin (KLH) (Ross et al., 1981). Shortly thereafter, Nairn and colleagues reported the production of serum antibodies that distinguished between the phospho- and dephospho-forms of G-substrate, a protein localized to cerebellar Purkinje cells and phosphorylated by cGMP-dependent protein kinase (Nairn et al., 1982). A synthetic heptapeptide, Arg-Lys-Asp-Thr-Pro-Ala-Leu, corresponding to a repeated sequence surrounding two phosphorylated threonyl residues in the intact protein, served as antigen. Rabbit antisera against a peptide-KLH conjugate were specific for the dephospho-form of G-substrate. Phospho-specific antibodies were prepared by immunization of rabbits with the purified phosphoprotein, phosphorylated in vitro to a stoichiometry of 2 mol/mol with cGMP-dependent protein kinase. Despite this initial success, other attempts in our laboratory to produce phospho-specific polyclonal antisera by immunization with the phospho-form of intact proteins were not very successful, probably because of two significant factors. First, many phosphorylated proteins are believed to undergo rapid dephosphorylation during immunization, regardless of the route of injection, leading to the loss of the desired phospho-epitope. Second, holoproteins generally contain multiple immunogenic epitopes; this decreases the probability that colonal dominance for a phospho-specific epitope will be obtained.

Taking a more direct approach utilizing phosphorylated and unphosphorylated forms of synthetic phosphopeptides, we developed a general protocol for the production of phosphorylation state-specific antibodies for substrates with established site(s) of phosphorylation (Czernik et al., 1991)). In early stages of our development of this methodology, phosphopeptides were routinely prepared by enzymatic phosphorylation (Czernik et al., 1991). Although this approach remains perfectly valid today, the preparation of synthetic phosphopeptides using Fmoc derivatives of phosphoamino acids has become the state-of-the-art (Czernik et al., 1995;Czernik et al., 1996). Likewise, we have examined the use of both polyclonal and monoclonal techniques for antibody production. Given the high success rate that we and others have obtained with the polyclonal technique, it has become the method of choice, because it is an easier and less costly method for the average laboratory. However, when appropriate, this approach can be readily adapted for monoclonal antibody production.

参考文献

1. Czernik AJ, Girault J-A, Nairn AC, Chen J, Snyder G, Kebabian J, Greengard P (1991) Production of phosphorylation state-specific antibodies. Methods Enzymol 201: 264-283.

2. Czernik AJ, Mathers J, Mische SM (1997) Phosphorylation state-specific antibodies. Neuromethods: Regulatory Protein Modification: Techniques & Protocols 30: 219-250.

3. Czernik AJ, Mathers J, Tsou K, Greengard P, Mische SM (1995) Phosphorylation state-specific antibodies: preparation and applications. Neuroprotocols 6: 56-61.

4. Lerner, R. A. Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature 299, 593-596. 1982.

5. Nairn AC, Detre JA, Casnellie JE, Greengard P (1982) Serum antibodies that distinguish between the phospho- and dephospho-forms of a phosphoprotein. Nature (Lond ) 299: 734-736.

6. Nestler, E. J. and Greengard, P. Protein Phosphorylation in the Nervous System. Nestler and Greengard. Protein Phosphorylation in the Nervous System. [8], 255-299. 1984. New York, Wiley. 

8. Sutcliffe JG, Shinnick TM, Green N, Lerner RA (1983) Antibodies that react with predetermined sites on proteins. Science 219: 660-666.

主营产品清单如下:

Item: Anti-ABCA4 (Rim Protein)
Category:  
Sub-Category:  
SKU/Catalog Number: 115-ABCA4
Datasheet:  click to view

 

SKU Price Formulation Applications Amount Qty
115-ABCA4 $325.00 Affinity purified, monoclonal antibody WB, IHC 100 µl

 

Phosphosolutions公司Anti-14-3-3 Protein产品代理


Phosphosolutions公司Anti-14-3-3 Protein产品代理

简要描述:公司概况

背景
基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。
蛋白质体学项目:Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。
PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。

our focus 专业特色
PhosphoSolutions公司专注于蛋白质组学中的一个(10-20

详细介绍

产品咨询

 公司概况

 
背景
基因工程– Phosphosolutions是*代可以完整描绘人体的遗传物质序列的企业。
蛋白质体学项目:Phosphosolutions是第二代试图将所有体内蛋白质表达出来的企业。
PhosphoSolutions公司—第三步我们将超越蛋白质体学 进而 专注于磷蛋白质。
 
our focus 专业特色
PhosphoSolutions公司专注于蛋白质组学中的一个(10-20%)含量的小部分磷蛋白质。磷蛋白是监管控制组蛋白质的关键,这一部分是被称为phosphosome蛋白质。磷蛋白被认为是在神经系统疾病如老年痴呆症和癌症方面的关键元素,实质上,phosphosome是蛋白质组学作物的精华。
 
公司目标
简明概述:我们要成为世界上的磷蛋白组的提供者。
方案#1, 特异性磷抗体:首先我们要准备磷蛋白组。在激活或磷酸化状态下磷蛋白组是蛋白质识别研究中的*关键工具。
Item: Anti-14-3-3 Protein
Category:  
Sub-Category:  
SKU/Catalog Number: 1433-P
Datasheet:  click to view

SKU Price Formulation Applications Amount Qty
1433-P $325.00 Affinity purified, polyclonal antibody WB 100 ul