The pSilencer 4.1-CMV siRNA Expression vectors are linearized with both BamH 1 and Hind III to facilitate directional cloning. They are also purified to remove the digested insert so that it cannot re-ligate with the vector. This greatly increases the percentage of clones bearing the hairpin siRNA template insert after ligation, reducing the time and effort required to screen clones.
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRA0341 | pSilencer 3.1-H1 neo | Ambion | RNAi载体 |
The pSilencer vectors employ RNA polymerase III （pol III） promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRA0340 | pSilencer 3.1-H1 hygro | Ambion | RNAi载体 |
The pSilencer vectors employ RNA polymerase III （pol III） promoters which generate large amounts of small RNA using relatively simple promoter and terminator sequences. They also include an antibiotic resistance gene that provides a mechanism to select for transfected cells that express the introduced DNA.
This Ambion® vector is for the expression of siRNA. It has an antibiotic resistance gene （hygromycin） to enable selection of transfected cells and features the human RNA polymerase III promoter （U6）. Sufficient reagents are provided for 20 reactions.
• Select transfected cells to enrich the population of cells expressing your siRNA
• Eliminate the need to synthesize RNA oligonucleotides for RNAi experiments
• Supplied linearized and ready for ligation
Compensate for Low Transfection Efficiencies and Perform Long-Term Studies
The use of mammalian siRNA expression vectors with antibiotic selectable markers conveys many benefits. Selectable markers can help compensate for poor plasmid transfection efficiencies seen with some cell lines. In these cases, only a fraction of the transfected cells express the siRNA, and reduction in target gene expression with even a potent siRNA can be difficult to detect. Use of a selectable marker and transient antibiotic selection permits.
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRA0337 | pSilencer1.0 | Ambion | RNAi载体 |
One of the biggest challenges in the use of RNA interference （RNAi） to
study gene function in mammalian systems is delivering the small interfering RNAs （siRNA） or siRNA expression vector into cells. The
pSilencer™ ade.0-CMV System employs adenoviral mediated gene
transfer—a well characterized and useful tool for the delivery of DNA
sequences both in vivo and in vitro. Once inside a mammalian cell, a
modified CMV promoter drives the expression of a hairpin siRNA for
RNAi studies. Using this kit, researchers can produce a replication-deficient adenovirus containing a hairpin siRNA template that targets their
gene of interest. It has been shown that adenoviral vectors are capable of
expressing functional siRNAs that enter the RNAi pathway and reduce
target gene expression in both tissue culture cells and animals （Xia 2002）.