pTWIN1 is an E. coli expression vector designed for protein purification or for the isolation of proteins with an N-terminal cysteine and/or a C-terminal thioester. A polylinker in the vector is designed for the in-frame fusion of a target gene between the modified Ssp DnaB and Mxe GyrA inteins. The presence of the chitin binding domain from Bacillus circulans facilitates purification.

• View sequence details
• Can be used with the IMPACT™ Protein Purification System
• Double-stranded vector
• 7,375 base pairs in length
• Ampicillin resistant

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