pTYB21 is an E. coli expression N-terminal fusion vector designed for in-frame insertion of a target gene into the polylinker downstream of the intein tag. This allows the N-terminus of the target protein to be fused to the intein tag. The self-cleavage activity of the intein allows the release of the target protein from the chitin-bound intein tag, resulting in a single column purification of the target protein.

• View sequence details
• Used in the IMPACT™ Protein Purification System
• Used in conjunction with a C-terminal fusion vector to test which fusion construction (N-terminal or C-terminal) maximizes the expression and yield of a target protein 
• 7,514 base pairs in length
• Ampicillin resistant