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pUC19 Vector
- View sequence details
- pUC19 is a commonly used cloning vector that conveys the Amp resistance.
- The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.
- pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1).
- NEB offers a selection of common cloning plasmids and DNAs for use as substrates.
精选视频
Traditional Cloning Workflow
产品信息
DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.
产品来源
pUC19 is isolated from E. coli ER2272 (dam+ dcm+ EcoK M–) by a standard plasmid purification procedure.
DNA 多聚接头序列
GENBANK 登录号
L09137
- 产品类别:
- DNA Plasmids & Substrates Products
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
N3041S -20 pUC19 Vector N3041SVIAL -20 1 x 0.05 ml 1 mg/ml
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N3041L -20 pUC19 Vector N3041LVIAL -20 1 x 0.25 ml 1 mg/ml
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- 特性和用法
应用
Cloning
拷贝数
High (100+)
复制起点
- pMB1 (high-copy mutant)
贮存溶液
10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C - 相关产品
相关产品
- pBR322 Vector
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
- 参考文献
- Yanisch-Perron, C., Vieira, J. and Messing, J. (1985). Gene. 33, 103-119.
工具 & 资源
- Web 工具
- DNA Sequences and Maps Tool
FAQs & 问题解决指南
- FAQs
- How homogeneous is the DNA/ how much is supercoiled?
- Are the plasmids cesium purified?
- What is the DNA sequence of this product?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
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质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- N3041S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- N3041S_L_v1_0381407
- N3041S_L_v1_0381412
- N3041S_L_v1_0381505
- N3041S_L_v1_0381510
- N3041S_L_v1_0391603
- N3041S_L_v1_0401608
- N3041S_L_v1_0401612
- N3041S_L_v1_0401704
- N3041S_L_v1_0411708
- N3041S_L_v1_0411802
- N3041L_v1_10017494
- N3041S_v1_10017495
- N3041L_v1_10035343
- N3041S_v1_10035341
- N3041L_v1_10050292
- N3041S_v1_10053892
- N3041S_v1_10062047
- N3041L_v1_10063250
- N3041S_v1_10081822
- N3041L_v1_10081821
- N3041S_v1_10096870
- N3041L_v1_10105200
- N3041S_v1_10121094
- N3041L_v1_10121097
- N3041S_v1_10132756
- N3041L_v1_10132755
- N3041L_v1_10144210
- N3041S_v1_10156442
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
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pUC19 Vector
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