| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRO0352 | pSUPER.Retro-GFP/Neo | Oligoengine | RNAi载体 |
Concentration: 0.5 mg/ml
Volume: 12 µl
Buffer: 10mM Tris-HCI pH 7.4, 1mM EDTA
Storage: Store at 4°C
Shipping: pSUPER.retro. is shipped to customers at ambient temperature to reduce shipping and
handling costs without affecting product quality and effectiveness.
Ambion RNAi载体，RNAi载体，pSilencer5.1-U6 Retro载体
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRA0580 | pSilencer5.1-U6 Retro | Ambion | RNAi载体 |
pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
pSilencer 5.1-U6 Retro uses the U6 promoter.
RNAi载体,RNAi-Ready pSIREN-Retro Q-ZsGreen载体
| 型号 | 载体名称 | 出品公司 | 载体用途 |
| VRC0347 | RNAi-Ready pSIREN-Retro Q-ZsGreen | Clontech | RNAi载体 |
Promoter: Human U6
Reporter: ZsGreen
Selection: prokaryotic—ampicillin
Replication: prokaryotic—Col E1 ori （low copy）, viral—SV40 ori
RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.