标签归档：rnai

pSilencer5.1-U6 Retro RNAi载体说明书

发表于由

 Ambion RNAi载体，RNAi载体，pSilencer5.1-U6 Retro载体

pSilencer5.1-U6 Retro

型号 载体名称 出品公司 载体用途
VRA0580 pSilencer5.1-U6 Retro Ambion RNAi载体
载体描述: 

pSilencer 5.1 Retro vectors harness retroviruses’ ability to stably integrate into the host genome of nearly all mitotically active cells and include a puromycin resistance gene for selection of cells that express the recombinant DNA. The pSilencer 5.1 Retro vectors enable researchers to study the long-term effects of reducing the expression of specific genes in cell culture models. This technology expands the capabilities of RNA interference research to address long-term alteration of gene expression.
pSilencer 5.1-U6 Retro uses the U6 promoter.

质粒图谱: 

RNAi-Ready pSIREN-Retro Q-ZsGreen

发表于由

 RNAi载体,RNAi-Ready pSIREN-Retro Q-ZsGreen载体

RNAi-Ready pSIREN-Retro Q-ZsGreen

型号 载体名称 出品公司 载体用途
VRC0347 RNAi-Ready pSIREN-Retro Q-ZsGreen Clontech RNAi载体
产品参数: 

Promoter: Human U6
Reporter: ZsGreen
Selection: prokaryotic—ampicillin
Replication: prokaryotic—Col E1 ori （low copy）, viral—SV40 ori

载体抗性: 
氨苄青霉素（Ampicillin）
载体描述: 

RNAi-Ready pSIREN-RetroQ-ZsGreen derives from our original gene silencing retroviral vector RNAi-Ready pSIREN-RetroQ. pSIREN-RetroQ-ZsGreen is a self-inactivating retroviral expression vector designed to express a small hairpin RNA （shRNA） using the human U6 promoter. This vector is provided as a linearized vector digested with BamH I and EcoR I. It is used for targeted gene silencing when an annealed DNA oligonucleotide encoding an appropriate shRNA is ligated into the vector. The pSIREN-RetroQ-ZsGreen construct can be transfected as a plasmid expression vector. For generation of active retrovirus for infecting hard-to-transfect cell lines, transfect the pSIREN-RetroQ-ZsGreen construct into a retroviral packaging cell line and collect the viral supernatant.

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