上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)
The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
Minimized adaptor-dimer contamination
High yields
Input RNA can be Total RNA
Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs
精选视频
A Breakthrough Method of Small RNA Sample Preparation for Next Gen Sequencing
观看其他视频
Need a custom/large volume order? Contact Us
Bulk packaging may also be available and requested for large recurring orders. Learn More
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)
The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
Minimized adaptor-dimer contamination
High yields
Input RNA can be Total RNA
Suitable for methylated small RNAs (e.g. piRNAs) as well as unmethylated small RNAs
精选视频
A Breakthrough Method of Small RNA Sample Preparation for Next Gen Sequencing
观看其他视频
Need a custom/large volume order? Contact Us
Bulk packaging may also be available and requested for large recurring orders. Learn More
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Acyclonucleotide Set
Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP). Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5).
产品信息
When used with Therminator DNA Polymerase (NEB #M0261), the concentration of acyclo-base analog is roughly equivalent to dideoxynucleotide concentrations used with Thermo Sequenase™ (see figure).
(50 ng/µl single-stranded M13mp18 DNA, 0.05 µM [32P]-S1224S primer, 1X ThermoPol Buffer, 50 µM dNTP, varying acyGTP and 0.05 units/µl Therminator) were mixed, incubated at 94°C for 5 minutes and then thermal cycled for 25 cycles at 94°C (30 seconds); 55°C (30 seconds); 72°C (30 seconds). Reaction products were separated on a denaturing polyacrylamide gel (QuickPoint, NOVEX, Inc.) and analyzed by autoradiography. Numbers above each lane give the molar ratio of acyGTP to dNTP in each lane (i.e., 50, 17, 5,6 and 1.9 µM acyGTP, respectively). Numbers to the side of the gel indicate the length of the primer extension product added to the primer.
产品类别:
Nucleotide Solutions Products
应用:
DNA Sequencing,
PCR,
PCR
试剂盒组成
试剂盒组成
本产品提供以下试剂或组分：
NEB #
名称
组分货号
储存温度
数量
浓度
N0460S
-20
AcycloATP
N0461AVIAL
-20
1 x 0.5 µmol
不适用
AcycloCTP
N0462AVIAL
-20
1 x 0.5 µmol
不适用
AcycloGTP
N0463AVIAL
-20
1 x 0.5 µmol
不适用
AcycloTTP
N0464AVIAL
-20
1 x 0.5 µmol
不适用
特性和用法
相关产品
相关产品
Therminator™ DNA Polymerase
注意事项
Thermo Sequenase™ is a trademark of GE Healthcare.
Addition of 50 μl of distilled or deionized (Milli-Q™) water will result in a final concentration of 10 mM acyNTP. The hydrated acyNTP solution should subsequently be stored at -20°C.
参考文献
Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Acyclonucleotide Set
Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP). Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5).
产品信息
When used with Therminator DNA Polymerase (NEB #M0261), the concentration of acyclo-base analog is roughly equivalent to dideoxynucleotide concentrations used with Thermo Sequenase™ (see figure).
(50 ng/µl single-stranded M13mp18 DNA, 0.05 µM [32P]-S1224S primer, 1X ThermoPol Buffer, 50 µM dNTP, varying acyGTP and 0.05 units/µl Therminator) were mixed, incubated at 94°C for 5 minutes and then thermal cycled for 25 cycles at 94°C (30 seconds); 55°C (30 seconds); 72°C (30 seconds). Reaction products were separated on a denaturing polyacrylamide gel (QuickPoint, NOVEX, Inc.) and analyzed by autoradiography. Numbers above each lane give the molar ratio of acyGTP to dNTP in each lane (i.e., 50, 17, 5,6 and 1.9 µM acyGTP, respectively). Numbers to the side of the gel indicate the length of the primer extension product added to the primer.
产品类别:
Nucleotide Solutions Products
应用:
DNA Sequencing,
PCR,
PCR
试剂盒组成
试剂盒组成
本产品提供以下试剂或组分：
NEB #
名称
组分货号
储存温度
数量
浓度
N0460S
-20
AcycloATP
N0461AVIAL
-20
1 x 0.5 µmol
不适用
AcycloCTP
N0462AVIAL
-20
1 x 0.5 µmol
不适用
AcycloGTP
N0463AVIAL
-20
1 x 0.5 µmol
不适用
AcycloTTP
N0464AVIAL
-20
1 x 0.5 µmol
不适用
特性和用法
相关产品
相关产品
Therminator™ DNA Polymerase
注意事项
Thermo Sequenase™ is a trademark of GE Healthcare.
Addition of 50 μl of distilled or deionized (Milli-Q™) water will result in a final concentration of 10 mM acyNTP. The hydrated acyNTP solution should subsequently be stored at -20°C.
参考文献
Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
Need a custom/large volume order? Contact Us 
Need a custom/large volume order? Contact Us 
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