上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NEBNext® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1)
Next® Multiplex Small RNA Library Prep Set for SOLiD™ (Set 1) |NEB酶试剂 New England Biolabs
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Next® Multiplex Small RNA Library Prep Set for Illumina® (Set 1) |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 1)
The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
- Minimized adaptor-dimer contamination
- High yields
- Input RNA can be Total RNA
- Suitable for methylated small RNAs (e.g., piRNAs) as well as unmethylated small RNAs
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Need a custom/large volume order? Contact Us
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Next® Multiplex Small RNA Library Prep Set for Illumina® (Set 2) |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
NEBNext® Multiplex Small RNA Library Prep Set for Illumina® (Set 2)
The unique workflow of the NEBNext® Small RNA library prep kits addresses the challenge of minimization of adaptor-dimers while achieving production of high-yield, diverse multiplex libraries in a simple protocol.
- Minimized adaptor-dimer contamination
- High yields
- Input RNA can be Total RNA
- Suitable for methylated small RNAs (e.g. piRNAs) as well as unmethylated small RNAs
精选视频
观看其他视频
Need a custom/large volume order? Contact Us
Bulk packaging may also be available and requested for large recurring orders.
Learn More
Acyclonucleotide Set | NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Acyclonucleotide Set
Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP).
Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5).
Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5).
产品信息
- 产品类别:
- Nucleotide Solutions Products
- 应用:
- DNA Sequencing,
- PCR,
- PCR
- 试剂盒组成
试剂盒组成
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
N0460S -20 AcycloATP N0461AVIAL -20 1 x 0.5 µmol 不适用 AcycloCTP N0462AVIAL -20 1 x 0.5 µmol 不适用 AcycloGTP N0463AVIAL -20 1 x 0.5 µmol 不适用 AcycloTTP N0464AVIAL -20 1 x 0.5 µmol 不适用
-
- 特性和用法
- 相关产品
相关产品
- Therminator™ DNA Polymerase
- 注意事项
- Thermo Sequenase™ is a trademark of GE Healthcare.
- Addition of 50 μl of distilled or deionized (Milli-Q™) water will result in a final concentration of 10 mM acyNTP. The hydrated acyNTP solution should subsequently be stored at -20°C.
- 参考文献
- Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
- Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
- Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
- Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2553.
- Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res.. 30(2), 605-613.
FAQs & 问题解决指南
- FAQs
- Are substrates terminated with 3′ acyclonucleosides resistant to 3′-5′ exonuclease activity?
- Which DNA polymerases incorporate acyclonucleotides?
- Can acyclonucleotides be used in Sanger sequencing?
- Can acyclonucleotides be used for sequencing-by-synthesis?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz - 出版物
- Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019) Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci; 6, 28. PubMedID: 31069234, DOI: 10.3389/fmolb.2019.00028
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- N0460S_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- N0460S_v0_10013356
- N0460S_v1_10013356
- N0460S_v1_10022005
- N0460S_v1_10048656
- N0460S_v1_10092480
- N0460S_v1_10123991
- N0460S_v1_10151064
- N0460S_v1_10154923
- N0460S_v1_10157552
- N0460S_v1_10163039
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
AcycloATP
-
AcycloCTP
-
AcycloGTP
-
AcycloTTP
-
Acyclonucleotide Set |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。
Acyclonucleotide Set
Acyclonucleotide Set contains four separate tubes of acyNTPs (acyATP, acyCTP, acyGTP and acyTTP).
Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5).
Acyclonucleotides (acyNTPs) act as chain terminators and are thus useful in applications that normally employ dideoxynucleotides such as DNA sequencing (1,2) and SNP detection (3). AcyNTPs are especially useful in applications with archaeon DNA Polymerases, more specifically with Therminator™ DNA Polymerase. Therminator DNA Polymerase is an engineered enzyme with an increased capacity to incorporate analogs with altered sugars, such as ribonucleotides, dideoxynucleotides, 2´ deoxynucleotides and especially acyclo-base analogs (4,5).
产品信息
- 产品类别:
- Nucleotide Solutions Products
- 应用:
- DNA Sequencing,
- PCR,
- PCR
- 试剂盒组成
试剂盒组成
本产品提供以下试剂或组分：
NEB # 名称 组分货号 储存温度 数量 浓度 -
N0460S -20 AcycloATP N0461AVIAL -20 1 x 0.5 µmol 不适用 AcycloCTP N0462AVIAL -20 1 x 0.5 µmol 不适用 AcycloGTP N0463AVIAL -20 1 x 0.5 µmol 不适用 AcycloTTP N0464AVIAL -20 1 x 0.5 µmol 不适用
-
- 特性和用法
- 相关产品
相关产品
- Therminator™ DNA Polymerase
- 注意事项
- Thermo Sequenase™ is a trademark of GE Healthcare.
- Addition of 50 μl of distilled or deionized (Milli-Q™) water will result in a final concentration of 10 mM acyNTP. The hydrated acyNTP solution should subsequently be stored at -20°C.
- 参考文献
- Sanger, F., Nicklen, S. and Coulson, A.R. (1977). Proc. Natl. Acad. Sci. USA. 74, 5463-5467.
- Trainor, G.L. (1996). U.S. Patent No. 5,558,991.
- Haff, L.A. and Simirnov, I.P. (1997). Genome Methods. 7, 378-388.
- Gardner, A.F. and Jack, W.E. (1999). Nucl. Acids Res.. 27, 2545-2553.
- Gardner, A.F. and Jack, W.E. (2002). Nucl. Acids Res.. 30(2), 605-613.
FAQs & 问题解决指南
- FAQs
- Are substrates terminated with 3′ acyclonucleosides resistant to 3′-5′ exonuclease activity?
- Which DNA polymerases incorporate acyclonucleotides?
- Can acyclonucleotides be used in Sanger sequencing?
- Can acyclonucleotides be used for sequencing-by-synthesis?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz - 出版物
- Gardner, A.F., Jackson, K.M., Boyle, M.M., Buss, J.A., Potapov, V., Gehring, A.M., Zatopek, K.M., Correa, I.R., Jr., Ong, J.L., Jack. W.E. (2019) Therminator DNA polymerase: Modified nucleotides and unnatural substrates Front Mol Biosci; 6, 28. PubMedID: 31069234, DOI: 10.3389/fmolb.2019.00028
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- N0460S_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- N0460S_v0_10013356
- N0460S_v1_10013356
- N0460S_v1_10022005
- N0460S_v1_10048656
- N0460S_v1_10092480
- N0460S_v1_10123991
- N0460S_v1_10151064
- N0460S_v1_10154923
- N0460S_v1_10157552
- N0460S_v1_10163039
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
AcycloATP
-
AcycloCTP
-
AcycloGTP
-
AcycloTTP
-