pLVX-shRNA2 is an HIV-1-based, lentiviral expression vector designed to express a small hair-pin RNA （shRNA） for RNA interference （RNAi） studies. Expression of your shRNA is driven by the RNA Pol III-dependent, human U6 promoter （PU6）, located just upstream of the MCS. pLVX-shRNA2 can be used as a plasmid expression vector and transfected into cells, or it can be packaged into viral particles and transduced into cells. Lentiviral particles derived from the vector allow the expression of shRNAs in virtually any cell type, including primary cells.

The pLVX-shRNA1 Vector is a lentiviral expression vector that can be used to generate high titers of lentivirus for transducing dividing or nondividing mammalian cells. Transduced target cells will express a user-generated shRNA from the U6 promoter for the purpose of RNAi-mediated gene silencing. pLVX-shRNA1 Vector can be packaged into high-titer, replication-incompetent, VSV-G-pseudotyped lentivirus upon transfection into 293T cells, using the Lenti-X™ HTX Packaging System. The resulting lentivirus can then be used to transduce virtually any mammalian cell type.