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EnGen® Spy Cas9 HF1
- High-fidelity variant of Spy Cas9 nuclease differing by four point mutations (N497A/R661A/Q695A/Q926A) reduces off-target cleavage
- Ideal for direct introduction of Cas9/sgRNA complexes
- Compatible with EnGen sgRNA Synthesis Kit, S. pyogenes (NEB #E3322S) and the EnGen Mutation Detection Kit (NEB #E3321S)