Unstained Protein Standard, Broad Range (10-200 kDa)

	库存
B9014S	10 X	6 ml	¥329.00	无	无	无	无	无
如何订购
Please enter a quantity for at least one size

The Unstained Protein Standard, Broad Range is a mixture of highly pure, recombinant proteins that resolves into 13 sharp bands from 10-200 kDa when electrophoresed and stained with Coomassie Brilliant Blue R-250 or other commercially available, ready-to-use protein stains.

Allows accurate molecular weight determination when performing SDS-PAGE analysis.
Direct loading, additional loading buffer and heat incubation not required
Recombinant proteins with no detectable protease contaminating activities
Optimal stability for up to 24 months

Unstained Protein Standard, Broad Range (10-200 kDa) |

Trypsin-digested BSA MS Standard (CAM-modified)

	库存
P7717S	不适用	150 gel lanes	¥1,619.00	有	无	无	无	无
P7717L	不适用	750 gel lanes	¥6,449.00	无	无	无	无	无
如何订购
Please enter a quantity for at least one size

This standard is a complex mixture of peptides produced by the tryptic digest of reduced and alkylated Bovine Serum Albumin (BSA).

Used to standardize MALDI-TOF or ESI mass spectrometers (TOF, Q-TOF, Ion Trap, or Orbitrap)
Standardization range of 500-2400 Da
Free of salts, glycerol and detergents
Supplied in dry format
Optimal stability for up to 24 months

Trypsin-digested BSA MS Standard (CAM-modified) |

	库存
P8108S	不适用	500 pmol	¥899.00	有	无	有	无	无
如何订购
Please enter a quantity for at least one size

hpc-standard2023年价格表–上海金畔生物

hpc-standards成立于2022年主要生产农药、兽药及其代谢物和稳定同位素标记衍生物的参考物质

特别告知：为保证产品质量与良好性能，所以在购买相应产品的同时我司会收取一定量的运输费用！！！本次报价有效时间为2023年5月12日—2023年12月31日。

货号	品名	规格	价格	品牌	货期	价格来源
672808	Acetamiprid	1x100mg	1889.8	hpc-standards	4-6周	上海金畔生物
672809	Chlorsulfuron	1x100mg	1647.8	hpc-standards	4-6周	上海金畔生物
672810	Ametryn	1x250mg	1229.8	hpc-standards	4-6周	上海金畔生物
672811	Amitraz	1x250mg	1229.8	hpc-standards	4-6周	上海金畔生物
672812	Amitrole	1x250mg	1273.8	hpc-standards	4-6周	上海金畔生物
672814	Atrazine	1x250mg	1097.8	hpc-standards	4-6周	上海金畔生物
672815	Benomyl	1x250mg	1361.8	hpc-standards	4-6周	上海金畔生物
672816	Bensulfuron-methyl	1x100mg	1647.8	hpc-standards	4-6周	上海金畔生物
672817	Benzylbenzoate	1x1000mg	1097.8	hpc-standards	4-6周	上海金畔生物
672819	Captan	1x250mg	1361.8	hpc-standards	4-6周	上海金畔生物
672820	Carbaryl	1x250mg	1097.8	hpc-standards	4-6周	上海金畔生物
672821	Carbendazim	1x250mg	921.8	hpc-standards	4-6周	上海金畔生物
672822	Carbofuran	1x250mg	1097.8	hpc-standards	4-6周	上海金畔生物
672824	Flutriafol	1x100mg	1735.8	hpc-standards	4-6周	上海金畔生物
672825	Chlorothalonil	1x250mg	1229.8	hpc-standards	4-6周	上海金畔生物
672826	Chlorpropham	1x250mg	921.8	hpc-standards	4-6周	上海金畔生物
672827	Oxyfluorfen	1x100mg	1361.8	hpc-standards	4-6周	上海金畔生物
672828	Tralkoxydim	1x250mg	1537.8	hpc-standards	4-6周	上海金畔生物
672829	Thifensulfuron-methyl	1x100mg	1889.8	hpc-standards	4-6周	上海金畔生物
672830	Triasulfuron	1x100mg	1097.8	hpc-standards	4-6周	上海金畔生物
672831	Tefluthrin	1x100mg	3187.8	hpc-standards	4-6周	上海金畔生物
672832	Mesotrione	1x100mg	1647.8	hpc-standards	4-6周	上海金畔生物
672833	Bispyribac sodium salt	1x100mg	3187.8	hpc-standards	4-6周	上海金畔生物
672834	Cymoxanil	1x100mg	1405.8	hpc-standards	4-6周	上海金畔生物
672835	Cypermethrin	1x100mg	987.8	hpc-standards	4-6周	上海金畔生物
672836	Cyromazine	1x250mg	921.8	hpc-standards	4-6周	上海金畔生物
672837	Diafenthiuron	1x250mg	1603.8	hpc-standards	4-6周	上海金畔生物
672838	Dichlobenil	1x250mg	921.8	hpc-standards	4-6周	上海金畔生物
672839	Dichlorvos	1x250mg	657.8	hpc-standards	4-6周	上海金畔生物
672840	Dicloran	1x250mg	613.8	hpc-standards	4-6周	上海金畔生物
672841	Difenoconazole	1x250mg	1471.8	hpc-standards	4-6周	上海金畔生物
672843	Dimethyl phthalate	1x1000mg	789.8	hpc-standards	4-6周	上海金畔生物
672845	Diphenylamine	1x250mg	415.8	hpc-standards	4-6周	上海金畔生物
672846	Sulfometuron-methyl	1x100mg	2923.8	hpc-standards	4-6周	上海金畔生物
672847	Diuron	1x250mg	921.8	hpc-standards	4-6周	上海金畔生物
672849	Fenobucarb	1x250mg	921.8	hpc-standards	4-6周	上海金畔生物
672850	Fenoxaprop-P-ethyl	1x250mg	1405.8	hpc-standards	4-6周	上海金畔生物
672851	Fenoxycarb	1x250mg	1471.8	hpc-standards	4-6周	上海金畔生物
672853	Fluazifop-P-butyl	1x100mg	1735.8	hpc-standards	4-6周	上海金畔生物
672854	Fluroxypyr	1x100mg	1471.8	hpc-standards	4-6周	上海金畔生物
672856	Furazolidone	1x250mg	811.8	hpc-standards	4-6周	上海金畔生物
672857	Glyphosate	1x250mg	1229.8	hpc-standards	4-6周	上海金畔生物
672858	Haloxyfop-P-methyl	1x100mg	2923.8	hpc-standards	4-6周	上海金畔生物
672859	Hexaconazole	1x100mg	1471.8	hpc-standards	4-6周	上海金畔生物
672860	Imazethapyr	1x100mg	1735.8	hpc-standards	4-6周	上海金畔生物
672861	Imidacloprid	1x100mg	1097.8	hpc-standards	4-6周	上海金畔生物
672862	Iprodione	1x100mg	921.8	hpc-standards	4-6周	上海金畔生物
672863	Isoprocarb	1x250mg	1097.8	hpc-standards	4-6周	上海金畔生物
672864	Linuron	1x100mg	657.8	hpc-standards	4-6周	上海金畔生物

关键字：672864 672863 672859 672862 672850

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer | NEB酶试剂 New England Biolabs

发表于2024年4月5日由Amresco中国官网

上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

The industry standard

Ideal for routine PCR applications
Exceptional value in terms of cost per unit
Mg-free buffer offers flexibility to adjust magnesium concentrations for use with microarray or DHPLC applications


货号	浓度	规格	目录价	北京	上海	广州	成都	苏州
M0320S	5,000 units/ml	400 units	¥669.00	有	无	有	无	无
M0320L	5,000 units/ml	2,000 units	¥2,699.00	无	无	无	无	有
如何订购
Please enter a quantity for at least one size

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer | NEB酶试剂 New England Biolabs Need a custom/large volume order? Contact Us

Bulk packaging may also be available and requested for large recurring orders.
Learn More

产品信息

Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity (1,2,3) and a 5´ flap endonuclease activity (4,5).

It is supplied with 10X Standard Taq (Mg-free) Reaction Buffer and MgCl₂. 10X Standard Taq (Mg-free) Reaction Buffer is detergent-free and designed to be compatible with existing assay systems.

重点

Isolated from a recombinant source
Supplied with 10X Reaction Buffer
Robust and reliable reactions
Tolerates a wide range of templates
Incorporates dUTP, dITP and fluorescently-labeled nucleotides

产品来源

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

产品类别:: Taq DNA Polymerase Products

应用:: Colony PCR,; A-tailing,; Multiplex PCR,; Specialty PCR,; Routine PCR,

产品组分信息

本产品提供以下试剂或组分：

NEB #

名称

组分货号

储存温度

数量

浓度

M0320S

-20

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer	M0320SVIAL	-20	1 x 0.08 ml	5,000 units/ml
Standard Taq (Mg-free) Reaction Buffer Pack	B9015SVIAL	-20	2 x 1.5 ml	10 X
Magnesium Chloride (MgCl₂) Solution	B9021SVIAL	-20	1 x 1.5 ml	25 mM

M0320L

-20

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer	M0320LVIAL	-20	1 x 0.4 ml	5,000 units/ml
Standard Taq (Mg-free) Reaction Buffer Pack	B9015SVIAL	-20	4 x 1.5 ml	10 X
Magnesium Chloride (MgCl₂) Solution	B9021SVIAL	-20	5 x 1.5 ml	25 mM

特性和用法
单位定义

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

反应条件

1X Standard Taq (Mg-free) Reaction Buffer Pack
Supplement with 1.5 mM MgCl₂ solution

1X Standard Taq (Mg-free) Reaction Buffer Pack
10 mM Tris-HCl
50 mM KCl
(pH 8.3 @ 25°C)

贮存溶液

10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C

热失活

否

分子量

理论上的: 94000 daltons

5′ – 3′ 核酸外切酶

Yes

3′ – 5′ 核酸外切酶

No

链置换

+

产物末端
- Single-base 3´ Overhangs
单位活性检测条件

1X ThermoPol^® Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 15 nM primed M13 DNA.

错配率

~ 285×10^-6bases
优势和特性
应用特性
- PCR
- Primer Extension
- DHPLC
- Microarray Analysis
- Colony PCR
相关产品
相关产品
- Taq 5X Master Mix
- Taq 2X Master Mix
- Taq PCR Kit
- Deoxynucleotide (dNTP) Solution Mix
- Deoxynucleotide (dNTP) Solution Set
- Standard Taq Reaction Buffer Pack
- Quick-Load® Taq 2X Master Mix
单独销售的组分
- Standard Taq (Mg-free) Reaction Buffer Pack
- Magnesium Chloride (MgCl₂) Solution
注意事项
1. 5’→3′ flap endonuclease destroys displaced strand.
参考文献
1. Chien, A., Edgar, D.B. and Trela, J.M. (1976). J. Bact.. 127, 1550-1557.
2. Kaledin, A.S., Sliusarenko, A.G. and Gorodetskii, S.I. (1980). Biokhimiya. 45, 644-651.
3. Lawyer, F.C. et al. (1993). PCRMethods and Appl.. 2, 275-287.
4. Longley, M.J., Bennett, S.E. and Mosbaugh D.W. (1990). NucleicAcids Res.. 18, 7317-7322.
5. Lyamichev, V., Brow, M.A. and Dahlberg, J.E. (1993). Science. 260, 778-783.
6. Saiki R.K. et al. (1985). Science. 230, 1350-1354.
7. Powell, L.M. et al. (1987). Cell. 50, 831-840.
8. Sun, Y., Hegamyer, G. and Colburn, N. (1993). Biotechniques. 15, 372-374.
9. Sarkar, G., Kapelner, S. and Sommer, S.S. (1990). NucleicAcids Res.. 18, 7465.

操作说明、说明书 & 用法

操作说明
1. PCR Protocol for Taq DNA Polymerase with Standard Taq (Mg-free) Buffer (M0320)
使用指南
- Activity of Restriction Enzymes in PCR Buffers
- Guidelines for PCR Optimization with Taq DNA Polymerase
- Guidelines for PCR Optimization with Thermophilic DNA Polymerases

工具 & 资源

选择指南
- DNA Polymerase Selection Chart
- Taq Buffer Selection Chart
- Thermophilic DNA Polymerases
Web 工具
- Tm Calculator

FAQs & 问题解决指南

FAQs
1. Does the presence of Ca²⁺ inhibit PCR?
2. Can Taq DNA Polymerase be used in other buffers?
3. When should Taq DNA Polymerase be used in a primer extension reaction or for PCR?
4. Why is the product a smear when visualized on an agarose gel?
5. Why is there no product when visualized on an agarose gel?
6. The product sequence doesn’t completely match the expected sequence. How can this result be improved?
7. Will the 5’→3′ flap endonuclease activity of Taq DNA Polymerase degrade primers?
8. How should I set up an amplification reaction using Taq DNA Polymerase?
9. What type of DNA ends result from a primer extension reaction or a PCR using Taq DNA Polymerase?
10. Can Taq DNA Polymerase be used for nick translation?
问题解决指南
- PCR Troubleshooting Guide
- Taq PCR Kit Troubleshooting Guide
实验技巧

Did you know most Taq reactions amplify more efficiently and robustly when you use a 68°C extension temperature?

引用 & 技术文献

引用文献

产品引用文献查找工具

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质控、安全 & 法规

质控分析

每一新批次的 NEB 产品都要经过质控，以满足指定的质量标准，对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息，可从此处查阅。
产品说明与变更通知
产品说明与变更通知

产品说明表包含产品的储存温度、有效期和规格，这些文件的命名规则如下：[货号]_[规格]_[版本]
- M0320S_L_v1
- M0320S_L_v2
CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控，这些文件的命名规则如下： [货号]_[规格]_[版本]_[Lot#]
- M0320S_L_v1_0141606
- M0320S_L_v1_0141612
- M0320S_L_v1_0141712
- M0320S_v1_10010817
- M0320L_v1_10013987
- M0320L_v1_10029609
- M0320L_v1_10033367
- M0320S_v1_10034143
- M0320L_v1_10040504
- M0320S_v1_10042497
- M0320S_v1_10054143
- M0320L_v1_10054144
- M0320S_v1_10055878
- M0320L_v1_10055893
- M0320S_v1_10063187
- M0320L_v2_10068564
- M0320S_v2_10074207
- M0320L_v2_10074205
- M0320S_v2_10095815
- M0320L_v2_10108347
- M0320S_v2_10116767
- M0320L_v2_10120896
- M0320L_v2_10122835
- M0320L_v2_10140673
- M0320S_v2_10146686
- M0320L_v2_10162786
SDS
以下 SDS 文件可以帮助您安全地使用该产品
- Taq DNA Polymerase with Standard Taq (Mg-free) Buffer
- Standard Taq (Mg-free) Reaction Buffer Pack
- Magnesium Chloride (MgCl₂) Solution

Amresco官网

Biolabs（NEB)酶 |Sigma-aldrich VWR 代理商Amresco中国代理商

标签归档：standard

Standard Taq Reaction Buffer Pack |NEB酶试剂 New England Biolabs

Standard Taq Reaction Buffer Pack

Unstained Protein Standard, Broad Range (10-200 kDa) |NEB酶试剂 New England Biolabs

Unstained Protein Standard, Broad Range (10-200 kDa)

Trypsin-digested BSA MS Standard (CAM-modified) |NEB酶试剂 New England Biolabs

Trypsin-digested BSA MS Standard (CAM-modified)

hpc-standard2023年价格表

hpc-standard2023年价格表–上海金畔生物

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer | NEB酶试剂 New England Biolabs

Taq DNA Polymerase with Standard Taq (Mg-free) Buffer

重点

产品来源

产品组分信息

单位定义

反应条件

贮存溶液

热失活

分子量

5′ – 3′ 核酸外切酶

3′ – 5′ 核酸外切酶

链置换

产物末端

单位活性检测条件

错配率

应用特性

相关产品

单独销售的组分

产品引用文献查找工具

产品说明与变更通知

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