标签归档：stem

Stem Cell 2013价格表

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Stem Cell 10061 Magnetic Colloid, 6 mL ￥1,562.00
Stem Cell 11000 4 channel Peristaltic pump, each ￥62,957.00
Stem Cell 11030 Red Magnet ￥9,688.00
Stem Cell 11050 Green Magnet ￥68,661.00
Stem Cell 11060 Blue Magnet ￥88,252.00
Stem Cell 11070 Black Magnet ￥142,839.00
Stem Cell 12021 0.1in Column Gravity Feed Kit, 10/pk ￥1,877.00
Stem Cell 12022 0.1in Column Pump Feed Kit, 10/pk ￥1,877.00
Stem Cell 12031 0.3in Column Gravity Feed Kit, 10/pk ￥2,780.00
Stem Cell 12032 0.3in Column Pump Feed Kit, 10/pk ￥2,780.00
Stem Cell 12051 0.5in Column Gravity Feed Kit, 10/pk ￥4,156.00
Stem Cell 12052 0.5in Column Pump Feed Kit, 10/pk ￥4,156.00
Stem Cell 12061 0.6in Column Gravity Feed Kit, 10/pk ￥8,326.00
Stem Cell 12062 0.6in Column Pump Feed Kit, 10/pk ￥8,326.00
Stem Cell 12231 Stopcocks, 10/pk ￥817.00
Stem Cell 12470 1.0in Column Pump Feed Kit, 1/pk ￥2,121.00
Stem Cell 13050 Anti-Biotin Tetramer, 2 mL ￥7,639.00
Stem Cell 13051 StemSep Mouse T Cell Kit 1x10e9 ￥8,728.00
Stem Cell 13052 StemSep Mouse CD4+ T Cell Kit, 1x10e9  ￥8,728.00
Stem Cell 13053 StemSep Mouse CD8+ T Cell Kit 1x10e9 ￥8,728.00
Stem Cell 13054 StemSep Mouse B Cell Kit, 1x10e9 ￥8,728.00
Stem Cell 13055 StemSep Mouse NK Cell Kit, 1x10e9 ￥8,728.00
Stem Cell 13056 StemSep Mouse Progenitor Kit, 1x10e9 ￥8,728.00
Stem Cell 13057 StemSep Mouse Dendritic Kit, 1x10e9 ￥8,728.00
Stem Cell 13058 StemSep Mu-Hu Chimera Depletion Kit ￥8,728.00
Stem Cell 13060 Anti-Biotin Tetramer, 10 mL ￥33,578.00
Stem Cell 13061 StemSep Mouse T Cell Kit, 5x10e9 cells ￥37,920.00
Stem Cell 13062 StemSep Mouse CD4+ T Cell Kit, 5x10e9 ￥37,920.00
Stem Cell 13063 StemSep Mouse CD8+ T Cell Kit, 5x10e9 ￥37,920.00
Stem Cell 13064 StemSep Mouse B Cell Kit, 5x10e9 ￥37,920.00
Stem Cell 13065 StemSep Mouse NK Cell Kit, 5x10e9 ￥37,920.00
Stem Cell 13066 StemSep Mouse Progenitor Kit, 5x10e9 ￥37,920.00
Stem Cell 13067 StemSep Mouse Dendritic Kit, 5x10e9 ￥37,920.00
Stem Cell 13068 Mu-Hu Chimera Depletion Kit, 5x10e9 ￥37,920.00
Stem Cell 13550 StemSep Anti-Phycoerytherin TAC,  2 mL ￥8,025.00
Stem Cell 13551 Rat Serum,  2 mL ￥1,003.00
Stem Cell 13560 StemSep Anti-Phycoerytherin TAC,  10 mL ￥33,578.00
Stem Cell 14002 StemSep Colloid, Medium Kit ￥444.00
Stem Cell 14003 StemSep Colloid, Large Kit ￥1,662.00
Stem Cell 14022 Special Anti-Human CD34 TAC, 400 uL ￥6,091.00
Stem Cell 14050 Anti-Human CD41 TAC, 200 uL ￥3,998.00
Stem Cell 14051 StemSep Human T Cells, 2 mL Kit ￥10,261.00
Stem Cell 14052 StemSep Human CD4+ T Cells, 2 mL, Kit ￥10,261.00
Stem Cell 14053 StemSep Human CD8+ T Cells, 2 mL ￥10,261.00
Stem Cell 14054 Stemsep Human B Cells, 2 mL Kit ￥10,261.00
Stem Cell 14055 StemSep Human NK Cells, 2 mL, Kit ￥10,261.00
Stem Cell 14056 StemSep Human Progenitor Cells, 2 mL Kit ￥10,261.00
Stem Cell 14057 StemSep Hu Prim Progenitor, 2 mL Kit  ￥10,261.00
Stem Cell 14058 StemSep Human Monocytes, 2 mL Kit ￥10,261.00
Stem Cell 14059 Special StemSep Human Basophil Cells Kit ￥12,583.00
Stem Cell 14060 Anti-Human CD41 TAC, 1 mL ￥16,896.00
Stem Cell 14061 StemSep Human T Cells, 10 mL, Kit ￥41,962.00
Stem Cell 14062 StemSep Human CD4+ T Cells, 10 mL Kit ￥41,962.00
Stem Cell 14063 StemSep Human CD8+ T Cells, 10 mL Kit ￥41,962.00
Stem Cell 14064 StemSep Human B Cells, 10 mL, Kit ￥41,962.00
Stem Cell 14065 StemSep Human NK Cells, 10 mL, Kit ￥41,962.00
Stem Cell 14066 StemSep Human Progenitor Cells 10 mL Kit ￥38,723.00
Stem Cell 14067 StemSep Hu Prim Progenitor, 10mL Kit ￥41,962.00
Stem Cell 14068 StemSep Human Monocyte Cells, 10 mL Kit ￥41,962.00
Stem Cell 14069 StemSep Human Basophil Cells, 10 mL ￥51,377.00
Stem Cell 14151 Special StemSep Human Granulocyte Kit ￥14,131.00
Stem Cell 14152 Special StemSep Hu Epithelial Tumor Kit ￥16,022.00
Stem Cell 14153 StemSep Human Dendritic Cells, 2 mL ￥10,261.00
Stem Cell 14154 Special StemSep Human CML Debulking Kit ￥16,452.00
Stem Cell 14155 StemSep Human Naive CD4+ T Cells, 2 mL ￥11,422.00
Stem Cell 14156 StemSep Human Eosinophil Cells, 2 mL ￥9,301.00
Stem Cell 14161 Special StemSep Human Granulocyte Kit ￥57,568.00
Stem Cell 14162 Special StemSep Hu Epithelial Tumo,10 mL ￥65,350.00
Stem Cell 14163 StemSep Human Dendritic Cells, 10 mL ￥41,962.00
Stem Cell 14164 Special StemSep Hu CML Debulking Kit ￥67,027.00
Stem Cell 14165 StemSep Human Naive CD4+ T Cells, 10 mL ￥50,489.00
Stem Cell 14166 StemSep Human Eosinophil Cells, 10 mL ￥34,166.00
Stem Cell 14231 Anti-Human CD25 TAC, 100 uL ￥2,838.00
Stem Cell 14233 Anti-Human CD45RA TAC, 100 uL ￥2,838.00
Stem Cell 14236 Anti-Human HLA-DR TAC, 100 uL ￥2,838.00
Stem Cell 14237 Anti-Human TCRab TAC, 100 uL ￥4,987.00
Stem Cell 14251 Anti-Human CD25 TAC, 200 uL ￥5,632.00
Stem Cell 14253 Anti-Human CD45RA TAC, 200 uL ￥5,632.00
Stem Cell 14256 Anti-Human HLA-DR TAC, 200 uL ￥5,632.00
Stem Cell 14257 Anti-Human TCRab TAC, 200 uL ￥9,975.00
Stem Cell 14551 Anti-Human CD32 Blocker, Neg Selection  ￥6,019.00
Stem Cell 14556 Anti-Human CD45RO Biotin ￥6,277.00
Stem Cell 14561 StemSep anti-Human CD32 Blocking, 4 mL ￥24,621.00
Stem Cell 14562 StemSep Biotinylated anti-Hu CD15, 3 mL ￥25,266.00
Stem Cell 14566 Anti-Human CD45RO Biotin, 1.5 mL ￥25,266.00
Stem Cell 14651 Special StemSep CD3 + T Cell Purge Kit ￥9,473.00
Stem Cell 14652 Special StemSep CD8 + T Cell Purge, Kit ￥9,473.00
Stem Cell 14653 Special StemSep Brst Carcinoma Purge Kit ￥36,688.00
Stem Cell 14661 Special StemSep CD3+T Cell Purge, 10mL ￥37,920.00
Stem Cell 14662 Special StemSep CD8+ T Cell Purge, 10mL ￥37,920.00
Stem Cell 14751 StemSep Human Positive T Cells, 1 mL ￥8,742.00
Stem Cell 14752 StemSep Human Positive CD4+ Cell, 1.0 mL ￥8,742.00
Stem Cell 14753 StemSep Human Positive CD8+T Cells, 1 mL ￥8,742.00
Stem Cell 14754 StemSep Human Positive CD19 Cells, 1 mL ￥8,742.00
Stem Cell 14755 StemSep Human Positive CD56 Cells, 1 mL ￥8,742.00
Stem Cell 14756 StemSep Human Positive CD34+ Cells, 1 mL ￥9,444.00
Stem Cell 14757 StemSep Positive CD138+ Cocktail, 1mL ￥8,742.00
Stem Cell 14758 StemSep Human Positive CD14 Cells, 1 mL ￥8,742.00
Stem Cell 14766 StemSep Human Positive CD34+ Cells, 5 mL ￥35,943.00
Stem Cell 14850 StemSep Biotin Positive Selection kit ￥6,248.00
Stem Cell 14851 Special StemSep FITC Positive Kit ￥6,248.00
Stem Cell 14852 StemSep PE Positive Selection Kit ￥6,248.00
Stem Cell 15021 RosetteSep Human T Cells, 2 mL ￥2,193.00
Stem Cell 15022 RosetteSep Human CD4+ T Cells, KIT ￥2,193.00
Stem Cell 15023 RosetteSep Human CD8+ T Cells, KIT ￥2,193.00
Stem Cell 15024 RosetteSep Human B Cells, 2 mL ￥2,193.00
Stem Cell 15025 RosetteSep Human NK Cells, 2 mL vial（s） ￥2,193.00
Stem Cell 15026 RosetteSep Cord Blood Progenitors, 2 mL ￥2,193.00
Stem Cell 15027 Rosettesep Bone Marrow Progenitors, 2 mL ￥5,088.00
Stem Cell 15028 RosetteSep Human Monocyte Cells, 2 mL ￥2,193.00
Stem Cell 15061 RosetteSep Human T Cells, 10 mL ￥8,498.00
Stem Cell 15061HLA RosetteSep™ HLA T Enrich Cocktail, 10mL ￥8,240.00
Stem Cell 15062 RosetteSep Human CD4+ T Cells, 10 mL ￥8,498.00
Stem Cell 15063 RosetteSep Human CD8+ T Cells, 10 mL ￥8,498.00
Stem Cell 15064 RosetteSep Human B Cells, 10 mL ￥8,498.00
Stem Cell 15064HLA RosetteSep™ HLA B Enrich Cocktail,  10mL ￥8,240.00
Stem Cell 15065 RosetteSep Human NK Cells, 10 mL ￥8,498.00
Stem Cell 15066 RosetteSep Cord Blood Progenitors, 10 mL ￥8,498.00
Stem Cell 15067 RosetteSep Bone Marrow Progenitor, 10 mL ￥19,548.00
Stem Cell 15068 RosetteSep Human Monocyte Cells, 10 mL ￥8,498.00
Stem Cell 15081HLA RosetteSep™ HLA T Enrich Cocktail, 40mL ￥29,680.00
Stem Cell 15084HLA RosetteSep™ HLA B Enrich Cocktail, 40 mL ￥29,680.00
Stem Cell 15122 RosetteSep CD45 Depletion, 2 mL ￥2,193.00
Stem Cell 15126 RosetteSep Debulking for Freezing, 2 mL ￥2,193.00
Stem Cell 15127 RosetteSep Extensive Tumor Enrich, 2 mL ￥5,088.00
Stem Cell 15128 RosetteSep Human Mesenchymal, 2 mL ￥2,193.00
Stem Cell 15129 RosetteSep Multi Myeloma Enrch Cocktail ￥5,589.00
Stem Cell 15162 RosetteSep CD45 Depletion, 10 mL ￥8,498.00
Stem Cell 15166 RosetteSep Debulking for Freezing, 10mL ￥8,498.00
Stem Cell 15167 RosetteSep Extensive Tumor Enrich, 10 mL ￥19,548.00
Stem Cell 15168 RosetteSep Human Mesenchymal, 10 mL ￥8,498.00
Stem Cell 15169 RosetteSep Multiple Myeloma, 10ml ￥21,511.00
Stem Cell 15223 RosetteSep Total Lymphocyte Enrich, 2 mL ￥2,193.00
Stem Cell 15230 RosetteSep Anti-Human IgE, 200 ul ￥1,548.00
Stem Cell 15263 RosetteSep Total Lymphocyte Enrich, 10mL ￥8,498.00
Stem Cell 15263HLA RosetteSep™ HLA Total Lymph Cktl 10mL ￥8,240.00
Stem Cell 15271HLA RosetteSep HLA Lymphoid Cell Enrich Kit  ￥11,537.00
Stem Cell 15272HLA RosetteSep HLA Myeloid Cell Enrich Kit  ￥11,537.00
Stem Cell 15276 RosetteSep Cord Blood Kit, Kit ￥6,134.00
Stem Cell 15283HLA RosetteSep™ HLA Total Lymph Cktl, 40 mL ￥28,132.00
Stem Cell 15361 RosetteSep Hu CD4+CD127lo Cocktail, 10mL ￥10,003.00
Stem Cell 15621 RosetteSep Human CD3 Depletion, 2 mL ￥1,218.00
Stem Cell 15622 RosetteSep Human CD4 Depletion, 2 mL ￥1,218.00
Stem Cell 15623 RosetteSep Human CD8 Depletion, 2 mL ￥1,218.00
Stem Cell 15624 RosetteSep Granulocyte Depletion, 2 mL ￥1,949.00
Stem Cell 15628 RosetteSep Monocyte CD36 Depletion, 2 mL ￥1,218.00
Stem Cell 15631 RosetteSep Cord Bld CD34 Pre-Enrich Kit ￥1,218.00
Stem Cell 15661 RosetteSep Human CD3 Depletion, 10 mL ￥4,858.00
Stem Cell 15662 RosetteSep Human CD4 Depletion, 10 mL ￥4,858.00
Stem Cell 15663 RosetteSep Human CD8 Depletion, 10 mL ￥4,858.00
Stem Cell 15664 RosetteSep Granulocyte Depletion, 10 mL ￥7,524.00
Stem Cell 15664HLA RosetteSep™ HLA Gran Dpln Cktl, 10 mL ￥7,309.00
Stem Cell 15668 RosetteSep Monocyte CD36 Depletion, 10mL ￥4,858.00
Stem Cell 15684HLA RosetteSep™ HLA Gran Dpln Cktl, 40 mL ￥24,119.00
Stem Cell 15705 RosetteSep DML, 100 mL ￥502.00
Stem Cell 15725 RosetteSep DMM, 100 mL ￥588.00
Stem Cell 15861 Rose/Easy CD4+CD127loCD25+ Kit for 200mL ￥10,935.00
Stem Cell 15861RF Rose/Robo CD4+CD127loCD25+ FilterTip Kit ￥11,451.00
Stem Cell 15862 RosetteSep/EasySep CD4CD25 Kit for 200mL ￥10,132.00
Stem Cell 15862RF RosetteSep/RoboSep CD4CD25 FilterTip Kit ￥11,035.00
Stem Cell 15864 Rose/Easy CD4+CD127loCD49d-CD25+, 200mL  ￥10,935.00
Stem Cell 15864RF Rose/Robo CD4+CD127lowCD25+ FiltTip Kit  ￥11,451.00
Stem Cell 16051 Special StemSep Rat T Cells, 2 mL ￥10,290.00
Stem Cell 16052 Special StemSep Rat CD4+ T Cells, 2 mL ￥10,290.00
Stem Cell 16053 Special StemSep Rat CD8+ T Cells, 2 mL ￥10,290.00
Stem Cell 16122 Special StemSep Rhesus CD8+Tetramer0.4mL ￥6,478.00
Stem Cell 16123 Special StemSep Rhesus CD4+Tetramer0.4mL ￥9,530.00
Stem Cell 16124 Special StemSep Rhesus CD3+Tetramer0.4mL ￥6,478.00
Stem Cell 16151 Special StemSep Rhesus T Cells, 2 mL ￥10,777.00
Stem Cell 16152 Special StemSep Rhesus CD4+TCells2 mLKit ￥13,486.00
Stem Cell 16153 Special StemSep Rhesus CD8+TCells2 mLKit ￥16,194.00
Stem Cell 16251 Special StemSep Rhesus TCell Purging Kit ￥16,194.00
Stem Cell 17531 SpinSep Density Medium, 100 mL ￥516.00
Stem Cell 18000 EasySep Violet Magnet ￥11,236.00
Stem Cell 18001 Big EasySep Magnet ￥16,854.00
Stem Cell 18001R Big EasySep Magnet for RoboSep ￥16,854.00
Stem Cell 18002 Easy50 EasySep Magnet ￥18,387.00
Stem Cell 18004 EasySep Multistand with Magnets ￥55,619.00
Stem Cell 18010 EasySep MultiStand ￥15,019.00
Stem Cell 18020 Big Easy Magnet O Rings w Fingers （x4） ￥946.00
Stem Cell 18051 EasySep Human CD3+, 10e9 cells ￥9,538.00
Stem Cell 18051HLA EasySep HLA CD3 Positive Selection Kit ￥8,774.00
Stem Cell 18051HLARF RoboSepHLA CD3 Positive Selection F Kit  ￥9,290.00
Stem Cell 18051RF RoboSep Human CD3+, 10e9 cells ￥10,054.00
Stem Cell 18052 EasySep Human CD4+, 10e9 cells ￥9,538.00
Stem Cell 18052RF RoboSep Human CD4+, 10e9 cells ￥10,054.00
Stem Cell 18053 EasySep Human CD8+, 10e9 cells ￥9,538.00
Stem Cell 18053RF RoboSep Human CD8+, 10e9 cells ￥10,054.00
Stem Cell 18054 EasySep Human CD19+, 10e9 cells ￥9,538.00
Stem Cell 18054RF RoboSep Human CD19+, 10e9 cells ￥10,054.00
Stem Cell 18055 EasySep Human CD56+, 10e9 cells ￥9,538.00
Stem Cell 18055RF RoboSep Human CD56+, 10e9 cells ￥10,054.00
Stem Cell 18056 EasySep Human CD34+, 5x10e9 cells ￥9,538.00
Stem Cell 18056RF RoboSep Human CD34+, 5x10e9 cells ￥10,054.00
Stem Cell 18058 EasySep Human CD14+, 10e9 cells ￥9,538.00
Stem Cell 18058RF RoboSep Human CD14+, 10e9 cells ￥10,054.00
Stem Cell 18062 EasySep CD4+CD25 T Cell Isolation Kit ￥13,312.00
Stem Cell 18062RF RoboSep CD4+CD25 T Cell FiltTip Kit ￥14,344.00
Stem Cell 18081 Whole Blood EasySep Human CD3+ KIT ￥8,312.00
Stem Cell 18081HLA EasySepHLA WB CD3 Positive Selection Kit ￥8,073.00
Stem Cell 18081HLARF RoboSepHLA WB CD3 Positive Slctn F Kit ￥9,363.00
Stem Cell 18081RF RoboSep Human CD3 Selection Whole Blood ￥9,602.00
Stem Cell 18082 Whole Blood EasySep CD4 Pos, Kit ￥8,312.00
Stem Cell 18082RF RoboSep Human CD4 Selection Whole Blood ￥9,602.00
Stem Cell 18083 Whole Blood EasySep CD8 positive, Kit ￥8,312.00
Stem Cell 18083RF RoboSep Human CD8 Selection Whole Blood ￥9,602.00
Stem Cell 18084 Whole Blood EasySep Human CD19+ KIT ￥8,312.00
Stem Cell 18084RF RoboSep Human CD19 Selection Whole Blood ￥9,602.00
Stem Cell 18085 Buffy Coat EasySep CD56 Positive Kit ￥8,312.00
Stem Cell 18085HLA EasySep HLA BC CD56 Positive Slctn Kit ￥8,073.00
Stem Cell 18085HLARF RoboSepHLA BC CD56 Pos Slctn Fltr Kit ￥9,363.00
Stem Cell 18085RF RoboSep Human CD56 Selection Buffy Coat ￥9,602.00
Stem Cell 18086 Whole Blood EasySep Human CD34+ ￥8,312.00
Stem Cell 18086RF RoboSep Human CD34 Selection Whole Blood ￥9,602.00
Stem Cell 18088 EasySep Buffy coat Human CD14+ ￥8,312.00
Stem Cell 18088RF RoboSep Human CD14 Selection Buffy Coat ￥9,602.00
Stem Cell 18096 EasySep Hu Cord Blood CD34 Pos Slctn Kit ￥9,745.00
Stem Cell 18096RF RoboSep Hu Cord Bld CD34 Pos Fltr Kit ￥10,905.00
Stem Cell 18098 EasySep Do it Yourself Selection Kit ￥6,349.00
Stem Cell 18098RF RoboSep Do-it-Yourself Selection Other ￥6,865.00
Stem Cell 18099 EasySep Hu Do it Yourself Selection Kit ￥7,532.00
Stem Cell 18099RF RoboSep Human Do-it-Yourself Selection ￥8,048.00
Stem Cell 18100 EasySep Multistand Big Magnets ￥74,207.00
Stem Cell 18150 EasySep Magnetic Particles, 1 mL ￥416.00
Stem Cell 18162 EasySep Hu Th17Cell Enrichment Kit ￥12,771.00
Stem Cell 18162RF RoboSep Human Th17 Cell Enrichment Kit ￥13,803.00
Stem Cell 18164 EasySep Hum. Memory B Cell Isolation Kit ￥12,771.00
Stem Cell 18164RF RoboSep Hum. Memory B Cell Isolation Kit ￥13,803.00
Stem Cell 18165 EasySep hESC/hiPSC SSEA-4 Pos. Sel. Kit ￥10,494.00
Stem Cell 18167 Easysep CD34+ kit from hES cells ￥10,494.00
Stem Cell 18184HLA EasySep HLA WB B Cell  Pos. Sel. Kit ￥8,073.00
Stem Cell 18184HLARF Robosep HLA WB B Cell Pos. Sel.w/fil.tip ￥9,363.00
Stem Cell 18231 EasySep CD25 Positive Kit ￥3,024.00
Stem Cell 18231RF RoboSep Human CD25 Selection Kit ￥3,540.00
Stem Cell 18250 EasySep SA Magnetic Nanoparticles 1 mL ￥430.00
Stem Cell 18251 EasySep Human Pan-CD25 Positive Select. ￥9,538.00
Stem Cell 18251RF RoboSep Human Pan-CD25 Positive ￥10,054.00
Stem Cell 18257 EasySep Human CD33 Pos Selection Kit ￥9,538.00
Stem Cell 18257RF RoboSep Hu CD33 Pos Sel Kit w/Fil Tips  ￥10,054.00
Stem Cell 18259 EasySep Human CD45 Depletion, KIT ￥9,538.00
Stem Cell 18259RF RoboSep Filter Tip Human CD45 Depletion  ￥10,054.00
Stem Cell 18287 EasySep WB CD33 Pos Select Kit w/lysis ￥8,312.00
Stem Cell 18287HLA EasySep HLA WB CD33 Positive Slctn Kit ￥8,073.00
Stem Cell 18287HLARF RoboSepHLA WB CD33 Pos Slctn Fltr Kit ￥9,363.00
Stem Cell 18287RF RoboSep WB CD33 Pos Selection Filter Kit ￥9,602.00
Stem Cell 18289 CD45 Depletion Kit for Whole Blood ￥8,312.00
Stem Cell 18289RF CD45 Depletion Kit for Whole Blood ￥8,828.00
Stem Cell 18352 EasySep Human Glycophorin A, 1 mL ￥9,538.00
Stem Cell 18352RF RoboSep Human Glycophorin A, Kit ￥10,054.00
Stem Cell 18356 EasySep Human EpCAM, 1 mL ￥9,538.00
Stem Cell 18356RF RoboSep Filter Tip Human EpCAM, KIT ￥10,054.00
Stem Cell 18357 EasySep Hu CD138 Positive Selection Kit ￥9,538.00
Stem Cell 18357RF RoboSep  Human CD138, 1x10e9 cells ￥10,054.00
Stem Cell 18358 EasySep Human CD10 Cells, 1 mL ￥9,538.00
Stem Cell 18359 EasySep Human MUC1 Cell 1mL ￥9,538.00
Stem Cell 18387 EasySep H WB CD138 Positive ￥8,312.00
Stem Cell 18387RF RoboSep Hu CD138 Selection Whole Blood ￥9,602.00
Stem Cell 18451 EasySep Human APC Selection Kit ￥7,739.00
Stem Cell 18451RF RoboSep Human APC Selection Kit （Filter） ￥8,255.00
Stem Cell 18452 EasySep Mouse APC Selection Kit ￥5,732.00
Stem Cell 18452RF RoboSep Mouse APC Selection Kit （Filter） ￥6,635.00
Stem Cell 18453 EasySep Other APC Selection Kit ￥5,346.00
Stem Cell 18453RF RoboSep Other APC Selection Kit （Filter） ￥5,862.00
Stem Cell 18454HLA EasySep HLA B Cell Positive Sel. Kit ￥7,197.00
Stem Cell 18454HLARF RoboSep HLA B Cell Pos. Sel. Kit w/fil t ￥7,713.00
Stem Cell 18520 Anti-Human CD32 Blocker, Pos Selection ￥924.00
Stem Cell 18551 EasySep Human PE, 10e9 cells ￥8,296.00
Stem Cell 18551RF RoboSep PE Selection Kit for Human Cells ￥8,812.00
Stem Cell 18552 EasySep Human FITC, 10e9 cells ￥4,916.00
Stem Cell 18552RF RoboSep FITC Selection Kit Human Cells ￥5,432.00
Stem Cell 18553 EasySep Human Biotin, 10e9 cells ￥4,916.00
Stem Cell 18553RF RoboSep Biotin Selection Kit Human Cells ￥5,432.00
Stem Cell 18554 EasySep Mouse PE, 10e9 cells ￥5,732.00
Stem Cell 18554RF RoboSep PE Selection Kit for Mouse Cells ￥6,635.00
Stem Cell 18555 EasySep Mouse FITC, 10e9 cells ￥4,916.00
Stem Cell 18555RF RoboSep FITC Selection Kit Mouse Cells ￥5,819.00
Stem Cell 18556 EasySep Mouse Biotin, 10e9 cells ￥4,916.00
Stem Cell 18556RF RoboSep Biotin Selection Kit Mouse Cells ￥5,819.00
Stem Cell 18557 EasySep （Other） PE, 10e9 cells ￥5,346.00
Stem Cell 18557RF RoboSep PE Selection Kit for Other Cells ￥5,862.00
Stem Cell 18558 EasySep （Other） FITC, 10e9 cells ￥4,471.00
Stem Cell 18558RF RoboSep FITC  Selection Kit Other Cells ￥4,987.00
Stem Cell 18559 EasySep （Other） Biotin, 10e9 cells vial ￥4,471.00
Stem Cell 18559RF RoboSep Biotin Selection Kit Other Cells ￥4,987.00
Stem Cell 18561 EasySep Human PE, 5 x 10e9 ￥33,635.00
Stem Cell 18564 EasySep Mouse PE, 5 x 10e9 ￥22,127.00
Stem Cell 18564RF RoboSep Mouse PE, 5 x 10e9 with Fil. Tip ￥29,858.00
Stem Cell 18653 EasySep Human Myeloid Cell Pos. Sel. Kit ￥9,538.00
Stem Cell 18653RF RoboSep Hu Myeloid  Sel. Kit w/fil. Tip ￥10,054.00
Stem Cell 18657 EasySep Human CD2 Pos Sel kit ￥9,538.00
Stem Cell 18657HLA EasySep HLA CD2 Positive Selection Kit ￥9,538.00
Stem Cell 18657HLARF RoboSep HLA CD2 Positive Slctn Fltr Kit ￥10,054.00
Stem Cell 18657RF RoboSep Human CD2 Positive Slctn Flt Kit ￥10,054.00
Stem Cell 18659 EasySep Human CD271 Pos Selection Kit ￥8,758.00
Stem Cell 18661 Special EasySep Human CD71 Selection Kit ￥9,538.00
Stem Cell 18661RF RoboSep Human CD71 FilterTip Select Kit ￥10,054.00
Stem Cell 18681 Whole Blood EasySep Human CD15+ Kit ￥8,312.00
Stem Cell 18681HLA EasySep HLA WB CD15 Positive Slctn Kit ￥8,073.00
Stem Cell 18681HLARF RoboSep HLA WB CD15 Pos Slctn Fltr Kit ￥9,363.00
Stem Cell 18681RF RoboSep Human CD15 Selection Whole Blood ￥9,602.00
Stem Cell 18682 Whole Blood EasySep CD66b positive KIT ￥8,312.00
Stem Cell 18682RF RoboSep Hu CD66b Selection Whole Blood ￥9,602.00
Stem Cell 18683 EasySep Hu WB Myeloid Pos Sel Kit w/RBC ￥8,312.00
Stem Cell 18683HLA EasySep HLA WB Myeloid Pos Slctn Kit ￥8,073.00
Stem Cell 18683HLARF RoboSepHLA WB Myeloid Pos Slctn Fltr Kit ￥9,363.00
Stem Cell 18683RF RoboSep Hu WB Myeloid Pos Sel Filter Kit ￥9,602.00
Stem Cell 18684 Whole Blood Lymphoid Pos Selection Kit ￥8,312.00
Stem Cell 18684HLA EasySep HLA WB Lymphoid Pstve Slctn Kit ￥8,073.00
Stem Cell 18684HLARF RoboSepHLA WB Lymph Pstve Slctn Fltr Kit ￥9,363.00
Stem Cell 18684RF RoboSep Filter Tip Lymphoid  Whole Blood ￥9,602.00
Stem Cell 18685 EasySep Hu WB CD20 Pos. Sel. Kit ￥8,312.00
Stem Cell 18685RF RoboSep Hu WB CD20 Pos.Sel Kit w/FilTip ￥9,602.00
Stem Cell 18687 EasySep Human WB CD2 KIT ￥8,312.00
Stem Cell 18687HLA EasySepHLA WB CD2 Positive Selection Kit ￥7,946.00
Stem Cell 18687HLARF RoboSepHLA WB CD2 Positive Slctn F Kit ￥9,236.00
Stem Cell 18687RF RoboSep FilterTip Human Whole Blood CD2 ￥9,602.00
Stem Cell 18721 EasySep Mouse CD25 Positive Select Kit ￥3,749.00
Stem Cell 18751 EasySep Mouse CD90.2 Positive Slctn Kit ￥10,869.00
Stem Cell 18751RF RoboSep Mouse CD90, KIT 2x10e9 cells ￥11,772.00
Stem Cell 18752 EasySep Mouse CD4 Positive Slctn Kit ￥10,869.00
Stem Cell 18752RF RoboSep Mouse CD4 Pos Slctn Fltr Kit ￥11,772.00
Stem Cell 18753 EasySep Mouse CD8a PE Positive Slctn Kit ￥10,869.00
Stem Cell 18753RF RoboSep Mouse CD8a PE Pos Slctn Fltr Kit ￥11,772.00
Stem Cell 18754 EasySep Mouse CD19 Positive Slctn Kit ￥10,869.00
Stem Cell 18754RF RoboSep Mouse CD19 Pos Slctn Fltr Kit ￥11,772.00
Stem Cell 18755 EasySep Mouse CD49b Pos Sel Kit ￥10,869.00
Stem Cell 18755RF RoboSep Mouse CD49b PosSel Flt Kit ￥11,772.00
Stem Cell 18756 EasySep Mouse SCA1 Positive Slctn Kit ￥10,869.00
Stem Cell 18756RF RoboSep Mouse Sca-1 Positive Slctn Kit F ￥11,772.00
Stem Cell 18757 EasySep Mouse CD117 （cKit） Pos Slctn Kit ￥10,869.00
Stem Cell 18757RF RoboSep Mouse CD117 （cKit） Pos Sel F Kit ￥11,772.00
Stem Cell 18758 EasySep Mouse CD11c Positive Slctn Kit ￥10,869.00
Stem Cell 18758RF RoboSep Mouse CD11c Pos Sel Fltr Tip Kit ￥11,772.00
Stem Cell 18761 EasySep Mouse CD25 Positive Select Kit ￥11,897.00
Stem Cell 18762 EasySep Mouse AA4.1 Positive Slctn Kit ￥11,897.00
Stem Cell 18768 EasySep CD11c & Spleen Dissoc Med Kit ￥11,554.00
Stem Cell 18768RF RoboSep CD11c, Spleen Dissoc FltrTip Kit ￥12,457.00
Stem Cell 18770 EasySep Mouse CD11b Pos Selection Kit ￥10,869.00
Stem Cell 18856 EasySep Mouse SCA1 Biotin Pos Sel Kit ￥10,275.00
Stem Cell 18856RF RoboSep Mouse SCA1 Biotin Pos Sel Kit ￥11,178.00
Stem Cell 19051 EasySep Negative Human T Cell, Kit ￥8,766.00
Stem Cell 19051HLA EasySep HLA Human T Cell Enrichment Kit ￥8,766.00
Stem Cell 19051HLARF RoboSep HLA Human T Cell Enrich Fltr Kit ￥9,282.00
Stem Cell 19051RF RoboSep Negative Human T Cell, 1 mL ￥9,282.00
Stem Cell 19052 EasySep Negative Human CD4 Kit ￥8,766.00
Stem Cell 19052RF RoboSep Negative Human CD4 w/fil.tip ￥9,282.00
Stem Cell 19053 EasySep Negative Human CD8, Kit ￥8,766.00
Stem Cell 19053RF RoboSep Negative Human CD8, 1 mL ￥9,282.00
Stem Cell 19054 EasySep Negative Human B Cell, Kit ￥8,766.00
Stem Cell 19054HLA EasySep HLA Human B Cell Enrichment Kit ￥8,766.00
Stem Cell 19054HLARF RoboSep HLA Human B Cell Enrich Fltr Kit ￥9,282.00
Stem Cell 19054RF RoboSep Negative Human B Cell, 1 mL ￥9,282.00
Stem Cell 19055 EasySep Negative Human NK, 1 mL ￥8,766.00
Stem Cell 19055RF RoboSep Negative Human NK ￥9,282.00
Stem Cell 19056 EasySep Negative Human Progenitor ￥8,766.00
Stem Cell 19056RF RoboSep Negative Human Progenitor ￥9,282.00
Stem Cell 19058 EasySep Hum. Monocyte Enrch Kit w/o CD16 ￥8,766.00
Stem Cell 19058RF RoboSep  Negative Human Monocyte, KIT ￥9,282.00
Stem Cell 19059 EasySep Human Monocyte Enrichment Kit  ￥8,766.00
Stem Cell 19059RF RoboSep Monocyte Enrich Kit w/FilterTips ￥9,282.00
Stem Cell 19061 EasySep Hu Myeloid DC Negative Sel Kit ￥9,154.00
Stem Cell 19061RF RoboSep Human Myeloid DC Enrichment Kit ￥10,186.00
Stem Cell 19062RF RoboSep Human pDC Enrich Kit w/ FltrTips ￥12,372.00
Stem Cell 19069 EasySep Human Basophil Enrichment Kit ￥8,766.00
Stem Cell 19069RF RoboSep Hu Basophil Enrich Fltr Tip Kit ￥9,282.00
Stem Cell 19150 EasySep Magnetic Particles, 1 mL ￥545.00
Stem Cell 19150.1 EasySep Magnetic Particles, 1 mL ￥530.00
Stem Cell 19153 EasySep Biotin Negative Selection, 1mL ￥5,876.00
Stem Cell 19154 EasySep Neg Human B Cell w/o CD43 Kit ￥8,766.00
Stem Cell 19154RF RoboSep Fltr Tip Neg Hu B Cell w/o CD43 ￥9,282.00
Stem Cell 19155 EasySep Human Naive CD4TCell Enrich,Kit ￥8,766.00
Stem Cell 19155RF EasySep Human Naive CD4TCell Enrich,Kit ￥9,282.00
Stem Cell 19157 EasySep Human Memory CD4 TCell, Kit ￥8,766.00
Stem Cell 19157RF RoboSep  Human Memory CD4 TCell, Kit ￥9,282.00
Stem Cell 19158 EasySep Hu Naive CD8 Enrich. Kit ￥8,766.00
Stem Cell 19158RF RoboSep Hu Naive CD8 Enrichment Kit ￥9,282.00
Stem Cell 19159 EasySep Hu Memory CD8 T Cell Enrich Kit ￥8,766.00
Stem Cell 19159RF RoboSep Hu Memory CD8 T Cell Enrich Kit ￥9,282.00
Stem Cell 19231 EasySep Hu CD4+CD127Lo T cell Enrich.Kit ￥8,766.00
Stem Cell 19231RF RoboSep HuCD4CD127Lo T enrich w/fil tip ￥9,282.00
Stem Cell 19232 EasySep CD4+CD127loCD49d- Kit ￥10,396.00
Stem Cell 19232RF RoboSep CD4+CD127loCD49d- FiltTip Kit ￥10,912.00
Stem Cell 19250 EasySep D Magnetic Particles, 1 mL ￥908.00
Stem Cell 19251 EasySep Human Pan-DC Pre-enrich, Kit ￥8,766.00
Stem Cell 19251RF RoboSep Fltr Tip Human Pan-DC Pre-enrich ￥9,282.00
Stem Cell 19254 EasySep Human Naïve B Cell Enrichment Ki ￥8,766.00
Stem Cell 19254RF RoboSep Hu Naive B Cell Kit w FilterTip ￥9,282.00
Stem Cell 19256 EasySep Negative Human Eosinophil ￥8,766.00
Stem Cell 19256RF RoboSep FltrTip Neg Human Eosinophil Kit ￥9,282.00
Stem Cell 19257 EasySep Human Neutrophil Enrichment Kit ￥11,275.00
Stem Cell 19257RF RoboSep Human Neutrophil Enrich Fltr Kit ￥11,791.00
Stem Cell 19350 EasySep M Prog Magnetic Microparticles ￥908.00
Stem Cell 19356 EasySep Hu Progenitor w Plaet Deplete ￥8,766.00
Stem Cell 19356RF RoboSep Hu Progenitor w Plaet Deplete ￥9,282.00
Stem Cell 19550 EasySep D Magnetic Particles, 1 mL ￥908.00
Stem Cell 19650 EasySep D2 Magnetic Particles ￥908.00
Stem Cell 19751 EasySep Mouse T cell enrich kit ￥10,865.00
Stem Cell 19751RF RoboSep Mouse Tcell enrich kit w/fil.tip ￥11,583.00
Stem Cell 19752 EasySep Mouse CD4+ T Cell Enrichment Kit ￥10,865.00
Stem Cell 19752RF RoboSep Mouse CD4+ Enrichment Kit ￥11,583.00
Stem Cell 19753 EasySep Mouse CD8+ T Cell Enrichment Kit ￥10,865.00
Stem Cell 19753RF RoboSep Mouse CD8+ Enrichment Kit ￥11,583.00
Stem Cell 19754 EasySep Negative Mouse B Cell Kit ￥10,865.00
Stem Cell 19754RF RoboSep Negative Mouse B Cell w/ fil.Tip ￥11,583.00
Stem Cell 19755 EasySep Mouse NK Cell Enrichment Kit ￥10,865.00
Stem Cell 19755RF RoboSep  Neg Mouse NK Cell, 400 uL Kit ￥11,583.00
Stem Cell 19756 EasySep Mouse Hem Prog Enrichment Kit ￥10,865.00
Stem Cell 19756RF RoboSep Mouse Hem Prog Enrich Kit w/FT ￥11,583.00
Stem Cell 19757 EasySep Mu Mammary Stemcell Enrich. Kit ￥20,116.00
Stem Cell 19758 EasySep Mouse Epithelial Cell Enrich.Kit ￥10,865.00
Stem Cell 19761 EasySep Mouse Monocyte Enrich Kit ￥10,865.00
Stem Cell 19761RF RoboSepMouse Monocyte Enrich.Kit w/fil.t ￥11,583.00
Stem Cell 19762 Mouse Neutrophil Negative Selection Kit ￥13,836.00
Stem Cell 19762RF Mouse Neutophil Robosep Filter Tip Kit ￥14,554.00
Stem Cell 19763 EasySep™ Mouse Pan-DC Enrichment Kit ￥14,713.00
Stem Cell 19763RF RoboSep™ Mouse Pan-DC Enrichment Kit ￥16,148.00
Stem Cell 19771 EasySep Mu Mesen Progen Cell Enrich Kit ￥11,603.00
Stem Cell 19772 EasySep Mu CD4+ TCell Pre-Enrch Kit NRS ￥10,347.00
Stem Cell 19782 EasySep Mu CD4+/CD25+ T Reg Cell Iso Kit ￥12,660.00
Stem Cell 19792 EasySep Mu CD4+/CD25+ T Reg Cell Iso Kit ￥49,442.00
Stem Cell 19951HLA EasySep HLA WB T Cell Enrichment Kit ￥5,909.00
Stem Cell 19951HLARF RoboSep HLA WB TCell Enrich Fltr Tip Kit ￥6,927.00
Stem Cell 19954HLA EasySep HLA WB B Cell Enrichment Kit ￥5,909.00
Stem Cell 19954HLARF RoboSepHLA WB B Cell Enrichment Fltr Kit ￥6,927.00
Stem Cell 19961HLA EasySepHLA WB Ttl Lymphocyte Enrich Kit ￥5,909.00
Stem Cell 19961HLARF RoboSepHLA WB Ttl Lymphocyte Enrch F Kit ￥6,927.00
Stem Cell 20000 RoboSep ￥820,000.00
Stem Cell 20000R RoboSep Refurbished ￥656,000.00
Stem Cell 20101 RoboSep Service Rack ￥3,941.00
Stem Cell 20103 Nalgene 250 mL Flask for RoboSep ￥115.00
Stem Cell 20104 RoboSep Buffer, 250 mL ￥272.00
Stem Cell 20106 6 Ft Ethernet Cross-over Cable ￥158.00
Stem Cell 20110 EasySep RBC Lysis Buffer, 10 mL ￥516.00
Stem Cell 20119 RoboSep Tip Head Polishing Compound ￥358.00
Stem Cell 20120 EasySep RBC Lysis Buffer, 100 mL ￥3,712.00
Stem Cell 20124 5X RoboSep Buffer ￥889.00
Stem Cell 20125 RoboSep Filter Tip Racks （box of 8） ￥760.00
Stem Cell 20144 EasySep™ Buffer ￥1,089.00
Stem Cell 20164 RoboSep Buffer 2 ￥616.00
Stem Cell 20200 RoboSep Limited Warranty, 1 yr trm ￥82,000.00
Stem Cell 20202 RoboSep Ltd Warranty w/ PM Pkg, 1 yr trm ￥123,000.00
Stem Cell 20203 RoboSep PM Pkg, 1 yr trm ￥82,000.00
Stem Cell 20208 RoboSep Warranty Re-instatement Fee ￥4,244.00
Stem Cell 20500 RoboSep Barcode Scanner ￥6,220.00
Stem Cell 19062 EasySep Human pDC Enrichment Kit ￥11,340.00
Stem Cell 07050 Trypan Blue, 100 mL ￥559.00
Stem Cell 07060 3 % Acetic Acid + Methylene Blue, 100 mL ￥473.00
Stem Cell 07906 HetaSep Solution, 100 mL ￥1,046.00
Stem Cell 15705 RosetteSep DML, 100 mL ￥502.00
Stem Cell 15725 RosetteSep DMM, 100 mL ￥588.00
Stem Cell 17531 SpinSep Density Medium, 100 mL ￥516.00
 

active motif Stem Cell CDy1 Dye 说明书

发表于由

世界*实验材料供应商 Active Motif上海金畔生物为其中国代理， Active Motif在一直是行业的*,一直为广大科研客户提供zui为的产品和服务,上海金畔生物一直秉承为中国科研客户带来的产品，的服务， Active Motif就是为了给广大科研客户带来更加完善的产品和服务,您的满意将是我们zui大的收获

Active Motif中国代理, Active Motif上海代理， Active Motif北京代理，Active Motif广东代理， Active Motif江苏代理Active Motif湖北代理,Active Motif天津,Active Motif黑龙江代理,Active Motif内蒙古代理,Active Motif吉林代理,Active Motif福建代理, Active Motif江苏代理, Active Motif浙江代理, Active Motif四川代理,

 

美国Active Motif公司是一家致力于细胞生物学和分子生物学研究用试剂的开发和生产的专业公司，是世界上的转录因子试剂生产商。其细胞生物学产品涉及：蛋白质转染试剂、一氧化氮定量试剂盒、转录因子ELISA试剂盒、细胞核提取试剂盒、染色试剂、细胞提取物、抗体等；分子生物学产品涉及：mRNA和总RNA的提取、E.coli表达系统、蛋白纯化系统、基因快速富集系统、DNA分离纯化系统、cDNA合成、RT-PCR试剂盒以及其他辅助试剂。

 

主要产品如下：

转录调控：

免疫沉淀试剂盒、DNA修复蛋白ELISA试剂盒、荧光素酶报告基因检测、核受体ELISA试剂盒、Gelshift 化学发光EMSA(电泳迁移率变动实验)、TransAM 转录因子ELISA试剂盒

表观遗传与染色质：

染色质组装试剂盒、染色质免疫沉淀试剂盒、DNA甲基化与富集试剂盒、组蛋白分析系列产品

 

Stem Cell CDy1 Dye

干细胞CDy1染料

活的染色成像和分选多能干细胞

 

活性基元干细胞CDy1染料为荧光显微镜和流式细胞仪提供了一种快速简便的方法，可选择性地对活的多能干细胞进行染色。由于CDy1是一种活细胞染料，因此不需要为了测试而牺牲珍贵的干细胞样品，与其他方法（如需要细胞固定的免疫染色）相比具有显着的优势。与其他活细胞染色技术相比，CDy1对多能干细胞具有高度特异性，在其他细胞（如饲养细胞或分化细胞）中不产生背景信号，如活碱性磷酸酶染色方法。染色是可逆的，不会影响细胞生长，形态或分化，因此非常适合干细胞富集和分选，或进行其他实验或复染方法。

与碱性磷酸酶活染色不同，CDy1对多能干细胞是特异性的。

科学技术与研究机构（A * STAR）和新加坡国立大学的科学家首先发现了这种染料作为多能干小鼠和人胚胎干细胞（mESCs，hESCs）的选择性染色，使得能够从饲养细胞中鉴定干细胞或混合文化。在这些研究中，在逆转录病毒感染Oct4，Sox2，Klf4和c-Myc转录因子之后，还使用CDy1染料来确定诱导性多能干细胞（iPSC）的重编程。他们的研究发表在2011年的自然协议1。随后的研究验证了使用干细胞CDγ1染料来鉴定和选择其他干细胞类型也已经发表。昆士兰大学Geoffrey W. Osborne实验室zui近发表的一篇文章中，干细胞CDy1染料用于富集小鼠大脑的神经干细胞2。另外，来自Robert Hawley实验室的研究表明使用干细胞CDy1染料来鉴定被认为是癌症干细胞的耐药性多发性骨髓瘤（MM）细胞的亚群2。要查看使用CDy1染料的已发布数据的详细信息，请单击下面的“ 参考”选项卡。

  1. Kang，NY 等人 （2011）Nat。Protoc。6，1044-1052。
  2. Vukovic，J。等人 （2013）Stem Cells Dev。DOI：10.1089 / scd.2012.0660。
  3. 霍利，TS 等。（2013）Am J Hematol。88，265-272。

图1：由干细胞CDy1染料染色的mESCs和MEFs的FACS分析。

将小鼠胚胎干细胞（mESC）和小鼠胚胎成纤维细胞（MEF）用干细胞CD11染料染色，然后使用侧向散射（SSC）和PE-德克萨斯 – 红色通道通过流式细胞术（FACS）进行分析。CDy1使得多能mESC（红色）与MEF（蓝色）明显分化和分离。用于CDy1染色的mESC的流式细胞术分析的协议信息在Kang N.-Y中被引用。等人。（2011）Nat Protoc。

图2：CDy1染色符合干细胞标记的表达。

将在小鼠胚胎成纤维细胞（MEF）饲养层上生长的小鼠胚胎干细胞（mESC）染色而不用Stem Cell CDy1染料固定。通过荧光显微镜观察CDy1染色。然后将细胞固定在4％多聚甲醛中，用Oct4抗体染色并用FITC缀合的二抗显色。图像显示mESC聚集体，但不是MEF饲养细胞，对CDy1（红色）和Oct4（绿色）都染色呈阳性。比例尺，100微米。图片由新加坡共和国新加坡国立大学YT Chang博士友情提供。

 

Name Format Cat No.

Stem Cell CDy1 Dye

干细胞CDy1染料

 50 µl 14001

 

 

zenogenpharma STEM-CELLBANKER说明书

发表于由

 

zenogenpharma STEM-CELLBANKER说明书

STEM-CELLBANKER ® EX GMP 级
（化学定义的冷冻保存溶液

 

    • 描述：

    • STEM-CELLBANKER ® EX GMP 级 (SCB EX) 是一种细胞冷冻保存解决方案，可扩展细胞治疗的可能性。SCB EX 是一种简单的成分，由经静脉注射证明的成分组成。它是一种含有 DMSO 的冷冻保存溶液和一种无异种物质的产品，配方不含血清和动物成分。SCB EX 还可以保存人类造血干细胞和人类脂肪来源的基质/干细胞。SCB EX 的制造符合日本、欧盟、美国和 PIC/S GMP 指南。所有将产品暴露在环境中的操作均在 A 级区域进行，以确保无菌。

      ※本产品已在 PMDA 内的 MF 中注册。
      ※本产品成分与HSC-BANKER ® GMP等级相同。

    • 应用：

    • 使用脂肪衍生基质/干细胞 (ADSC) 进行冷冻保存性能测试

      测试：EMO生物医学

    • 1. 细胞活力测试

      ADSC 与 SCB EX 一起冷冻保存 4、12、24 和 48 周。解冻后立即评估细胞活力（左图）和解冻后过夜（右图）。用 SCB EX 冷冻保存的细胞即使在长期冷冻保存后也显示出高活力，即使在过夜休息后也保持高活力。

      2. 细胞恢复测试

      ADSC 与 SCB EX 一起冷冻保存 4、12、24 和 48 周。然后，我们评估解冻后过夜的细胞附着（细胞恢复；左图）和解冻和培养 72 小时后的细胞生长速率（倍增时间；右图）。结果表明，即使经过长期冷冻保存，细胞恢复和细胞生长速度也没有受到影响。

      3. 小区身份测试

      ADSC 与 SCB EX 一起冷冻保存 4、12、24 和 48 周。然后，我们使用流式细胞仪的阳性和阴性标记评估解冻后的细胞身份。结果表明，即使在长期冷冻保存后，用每种标记物标记的细胞百分比也不受影响。

      阳性标记；CD29、CD73、CD90 和 CD105
      阴性标记；CD11b、CD19、CD34、CD45 和 HLA-DR

      阳性标记数据

      4. 细胞功能测试
      　（IDO 量化和抑制 T 细胞增殖测定）

      ADSC 与 SCB EX 一起冷冻保存 4、12、24 和 48 周。然后，我们评估了解冻后 ADSC 的细胞功能。结果表明，即使经过长期冷冻保存，在MSCs存在下IDO表达和T细胞增殖抑制率均不受影响。

      ※吲哚胺-2,3-双加氧酶（IDO）

    • 协议（CELLBANKER ®系列）

    • 冷冻

      为获得最佳结果，冷冻保存的细胞应处于对数生长期。可以替代类似或标准的冷冻方案。

      重要提示：最佳方案可能会随细胞类型而变化。

      解冻

      ※如果成活率低，请尝试使用冷冻箱等慢速冷冻方法。

      • 从储存中取出冷冻细胞，在 37 ℃ 振荡水浴中快速解冻。

      • 立即将每 1ml 细胞与 10ml *细胞培养基稀释并轻轻混合。

      • 轻轻离心沉淀细胞（3-5 分钟，1,000~2,000rpm，4 ℃）。取出上清液抽吸器。

      • 用适当体积的*细胞培养基轻轻悬浮细胞。并放入培养瓶中。

      • 根据标准协议继续进一步的培养程序。

      • 检查并确保细胞培养物无污染、健康状况和适当融合等。

      • 进行细胞计数以确定细胞的活力

      • 轻轻离心沉淀细胞（3-5 分钟，1,000~2,000rpm，4℃）。使用吸气器去除上清液。

      • 用 CELLBANKER 冷冻保存培养基轻轻悬浮细胞（1 ml 用于 5×105 – 5×106 细胞）。

      • 将细胞悬液以 1ml 等分试样分装到已标有细胞系名称、细胞浓度、传代日期和其他重要信息的冷冻保存小瓶中。

      • 将小瓶直接置于-80℃保存。如有必要，在小瓶冷冻至少 24 小时后，将冷冻小瓶转移到液氮储罐中。

    • 储存和稳定性：

    • 储存于 2 至 8 或低于 -20 摄氏度。该产品在此储存温度下自生产之日起可保持稳定 3 年。