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System Biosciences（SBI）开发创新的研究工具为载体传递技术，microRNA研究exosome研究，基因组工程工具，包括smartnuclease™CRISPR-Cas9系统和precisionx™HR打靶载体。

System Biosciences (SBI) develops innovative research tools for Vector delivery technologies, MicroRNA research, Exosome research, and Genome Engineering tools, including the CRISPR-Cas9 SmartNuclease™ system and PrecisionX™ HR targeting vectors. Highlights: Vector Technologies: • Powerful lentivector systems • One-step transfection piggyBac transposons • Single-copy gene additions with phiC31 integrase vectors • Sustained expression without integration with Minicircle DNA plasmids Exosome Research Tools: • Isolate exosomes with ExoQuick and ExoQuick-TC • Exo-Flow- Immunopurify exosomes on magentic beads and anlayze by FACS • Culture cells in exosome-depleted FBS • Verify exosome recovery with antibodies • Quantitate exosomes with ELISAs Genome Engineering: • RNA-guided Cas9 SmartNuclease system • Fast and efficient mutation correction / gene editing • HR donor vectors for knock-in, knock-out & tagging • High-throughput, targeted integration with PinPoint technology Stem Cell Research: • iPSC reprogramming systems • Growth factors and media • Pluripotency confirmation kits and antibodies • iPS celllines, normal and disease models • Promoter lineage reporters in lentivectors Non-coding RNA: • LncRNA qPCR profiling kits • miRNome and OncoMir qPCR profiling systems • Lenti-miR Precursor clone libraries – arrayed and pooled • miRZip anti-miR clones for stable miRNA inhibition Exo-NGS Custom Service: • Provide serum, tissue culture media, or other patient biofluids to SBI • SBI isolates exosomes, purifies RNA and generates high quality NGS libraries • Sequencing performed using Illumina MiSeq and HiSeq platforms • Comprehensive data analysis, including generation of heat maps and top hits, provided • NGS data loaded onto a custom UCSC Genome Browser for convenient viewing

SBI的产品组合

• 慢病毒技术
• 外来体工具
• MicroRNA研究
• 基因组工程
• 干细胞技术

Vibrant Blue and Red Detection Systems

Alkaline phosphatase (AP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides and proteins under alkaline conditions. The Dual-Color Alkaline Phosphatase Staining Kit is a histochemical assay for use on cells grown in tissue culture wells or dishes. It uses a solution containing substrate and color dye as the colorimetric read-out for the enzymatic activity. The AP-positive, undifferentiated stem cells stain red or blue. The stain develops quickly, does not readily fade, and can be analyzed 15 to 30 minutes after application to the cultured cells. For added convenience, the stained samples can be kept at 4°C for up to one week for analysis.

T cell research tools

• The Lentivector constructs can be packaged with SBI's pPACK-H1 packaging mix (cat#LV500A-1) and virus concentrated using PEG-it (cat#LV810A-1).
• The piggyBac transposon constructs can be integrated into target cells using the Super PiggyBac transposase (cat#PB200PA-1).
• To turn on the the Cumate Switch constructs, use the 10,000x Cumate solution (cat#PBQM100A-1).

T细胞研究工具产品信息如下：