上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
T7 DNA Polymerase (unmodified)
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The high polymerization rate of the enzyme makes long incubations unnecessary.
- Second strand synthesis in site-directed mutagenesis protocols
- Gap filling reaction (no strand displacement)
- T7 DNA Polymerase is not suitable for DNA sequencing
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
产品信息
产品来源
T7 DNA Polymerase consists of two subunits: T7 gene 5 protein (84 kilodaltons) and E.coli thioredoxin (12 kilodaltons) (1,4-7). Each protein is cloned and overexpressed in a T7 expression system in E. coli (4).
- 产品类别:
- DNA Manipulation Products
- 应用:
- Isothermal Amplification,
- PCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0274S -20 T7 DNA Polymerase (unmodified) M0274SVIAL -20 1 x 0.03 ml 10,000 units/ml T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
-
M0274L -20 T7 DNA Polymerase (unmodified) M0274LVIAL -20 1 x 0.15 ml 10,000 units/ml T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
-
- 特性和用法
单位定义
One unit is defined as the amount of enzyme that will incorporate10 nmoles of dNTP into acid insoluble material in 30 minutes at 37°C .
反应条件
1X T7 DNA Polymerase Reaction Buffer
Supplement with Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C1X T7 DNA Polymerase Reaction Buffer
20 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.5 @ 25°C)贮存溶液
50 mM KPO4
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7 @ 25°C热失活
75°C for 20 min
5′ – 3′ 核酸外切酶
No
3′ – 5′ 核酸外切酶
Yes
链置换
No
单位活性检测条件
100 mM KCl, 20 mM Tris-HCl (pH 7.6), 6 mM MgCl2 , 0.5 mM DTT, 0.1 mM EDTA, 50 ug/ml Recombinant Albumin, 150 μM dNTPs including [3H]-dTTP and 70 µg/ml denatured herring sperm DNA.
错配率
~ 15×10-6bases
- 优势和特性
应用特性
- Second strand synthesis in site-directed mutagenesis protocols (8).
- 相关产品
相关产品
- Deoxynucleotide (dNTP) Solution Mix
- Deoxynucleotide (dNTP) Solution Set
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
单独销售的组分
- 重组白蛋白, 分子生物学级(不含动物成分)
- 注意事项
- The high polymerization rate of the enzyme makes long incubations unnecessary.
- T7 DNA Polymerase is not suitable for DNA sequencing.
- 参考文献
- Hori, K. et al. (1979). J. Biol. Chem.. 254, 11598-11604.
- Engler, M.J. et al. (1983). J. Biol. Chem.. 258, 11165-11173.
- Nordstrom, B. et al. (1981). J. Biol. Chem.. 256, 3112-3117.
- Studier, F.W. et al. (1990). Goeddel, D.V.(Ed.), In Methods Enzymol.. 185, 60-89. San Diego: Academic Press.
- Grippo, P. and Richardson, C.C. (1971). J. Biol. Chem. 246, 6867-6873.
- Modrich, P. and Richardson, C.C. (1975). J. Biol. Chem. 250, 5515-5522.
- Adler, S. and Modrich, P. (1979). J. Biol. Chem. 254, 11605-11614.
- Bebenek, K. and Kunkel, T.A. (1989). Nucl. Acids Res.. 17, 5408.
- Mattila, P., et al. (1991). Nucleic Acids Res.. 19, 4967-4973.
操作说明、说明书 & 用法
- 使用指南
- Activity of DNA Modifying Enzymes in rCutSmart™ Buffer
工具 & 资源
- 选择指南
- DNA Polymerase Selection Chart
FAQs & 问题解决指南
- FAQs
- Can T7 DNA Polymerase be used in other NEBuffers, including rCutSmart?
- Can T7 DNA Polymerase be used to blunt DNA?
- Can T7 DNA Polymerase be used to fill in 3′ overhangs?
- Can T7 DNA Polymerase be used to remove 5′ overhangs?
- Can T7 DNA Polymerase be heat inactivated?
- Are deoxynucleotides needed to remove a 3′ overhang using T7 DNA Polymerase?
- When should native T7 DNA Polymerase be used?
- What is the oligonucleotide-directed mutagenesis without phenotypic selection method?
- Can the native T7 DNA Polymerase be used for sequencing?
- Does NEB carry modified T7 Polymerase?
- Can T7 DNA Polymerase be used in labeling reactions and partial fill in reactions?
- Are NEB DNA Polymerases supplied with dNTPs?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- M0274S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- M0274S_L_v1_0111508
- M0274S_L_v1_0111603
- M0274S_L_v1_0111609
- M0274S_L_v1_0111703
- M0274S_L_v1_0111803
- M0274L_v1_10012703
- M0274S_v1_10021765
- M0274L_v1_10021767
- M0274L_v1_10039918
- M0274S_v1_10040917
- M0274L_v1_10057374
- M0274S_v1_10059218
- M0274L_v1_10075649
- M0274L_v1_10081788
- M0274S_v1_10091672
- M0274L_v1_10091094
- M0274S_v1_10107595
- M0274S_v1_10114913
- M0274S_v1_10123097
- M0274L_v1_10131164
- M0274S_v1_10135910
- M0274L_v1_10139934
- M0274L_v1_10152153
- M0274S_v1_10161568
- M0274L_v1_10161623
- M0274S_v1_10163746
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
T7 DNA Polymerase (unmodified)
-
T7 DNA Polymerase Reaction Buffer
-
重组白蛋白, 分子生物学级
-
T7 DNA Polymerase (unmodified) |NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
T7 DNA Polymerase (unmodified)
T7 DNA Polymerase catalyzes the replication of T7 phage DNA during infection. The high polymerization rate of the enzyme makes long incubations unnecessary.
- Second strand synthesis in site-directed mutagenesis protocols
- Gap filling reaction (no strand displacement)
- T7 DNA Polymerase is not suitable for DNA sequencing
- Isolated from a recombinant source
- Supplied with 10X Reaction Buffer
产品信息
产品来源
T7 DNA Polymerase consists of two subunits: T7 gene 5 protein (84 kilodaltons) and E.coli thioredoxin (12 kilodaltons) (1,4-7). Each protein is cloned and overexpressed in a T7 expression system in E. coli (4).
- 产品类别:
- DNA Manipulation Products
- 应用:
- Isothermal Amplification,
- PCR
- 产品组分信息
产品组分信息
本产品提供以下试剂或组分:
NEB # 名称 组分货号 储存温度 数量 浓度 -
M0274S -20 T7 DNA Polymerase (unmodified) M0274SVIAL -20 1 x 0.03 ml 10,000 units/ml T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
-
M0274L -20 T7 DNA Polymerase (unmodified) M0274LVIAL -20 1 x 0.15 ml 10,000 units/ml T7 DNA Polymerase Reaction Buffer B0274AVIAL -20 1 x 1.5 ml 10 X Recombinant Albumin, Molecular Biology Grade B9200SVIAL -20 1 x 0.6 ml 20 mg/ml
-
- 特性和用法
单位定义
One unit is defined as the amount of enzyme that will incorporate10 nmoles of dNTP into acid insoluble material in 30 minutes at 37°C .
反应条件
1X T7 DNA Polymerase Reaction Buffer
Supplement with Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C1X T7 DNA Polymerase Reaction Buffer
20 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.5 @ 25°C)贮存溶液
50 mM KPO4
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7 @ 25°C热失活
75°C for 20 min
5′ – 3′ 核酸外切酶
No
3′ – 5′ 核酸外切酶
Yes
链置换
No
单位活性检测条件
100 mM KCl, 20 mM Tris-HCl (pH 7.6), 6 mM MgCl2 , 0.5 mM DTT, 0.1 mM EDTA, 50 ug/ml Recombinant Albumin, 150 μM dNTPs including [3H]-dTTP and 70 µg/ml denatured herring sperm DNA.
错配率
~ 15×10-6bases
- 优势和特性
应用特性
- Second strand synthesis in site-directed mutagenesis protocols (8).
- 相关产品
相关产品
- Deoxynucleotide (dNTP) Solution Mix
- Deoxynucleotide (dNTP) Solution Set
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
单独销售的组分
- 重组白蛋白, 分子生物学级(不含动物成分)
- 注意事项
- The high polymerization rate of the enzyme makes long incubations unnecessary.
- T7 DNA Polymerase is not suitable for DNA sequencing.
- 参考文献
- Hori, K. et al. (1979). J. Biol. Chem.. 254, 11598-11604.
- Engler, M.J. et al. (1983). J. Biol. Chem.. 258, 11165-11173.
- Nordstrom, B. et al. (1981). J. Biol. Chem.. 256, 3112-3117.
- Studier, F.W. et al. (1990). Goeddel, D.V.(Ed.), In Methods Enzymol.. 185, 60-89. San Diego: Academic Press.
- Grippo, P. and Richardson, C.C. (1971). J. Biol. Chem. 246, 6867-6873.
- Modrich, P. and Richardson, C.C. (1975). J. Biol. Chem. 250, 5515-5522.
- Adler, S. and Modrich, P. (1979). J. Biol. Chem. 254, 11605-11614.
- Bebenek, K. and Kunkel, T.A. (1989). Nucl. Acids Res.. 17, 5408.
- Mattila, P., et al. (1991). Nucleic Acids Res.. 19, 4967-4973.
操作说明、说明书 & 用法
- 使用指南
- Activity of DNA Modifying Enzymes in rCutSmart™ Buffer
工具 & 资源
- 选择指南
- DNA Polymerase Selection Chart
FAQs & 问题解决指南
- FAQs
- Can T7 DNA Polymerase be used in other NEBuffers, including rCutSmart?
- Can T7 DNA Polymerase be used to blunt DNA?
- Can T7 DNA Polymerase be used to fill in 3′ overhangs?
- Can T7 DNA Polymerase be used to remove 5′ overhangs?
- Can T7 DNA Polymerase be heat inactivated?
- Are deoxynucleotides needed to remove a 3′ overhang using T7 DNA Polymerase?
- When should native T7 DNA Polymerase be used?
- What is the oligonucleotide-directed mutagenesis without phenotypic selection method?
- Can the native T7 DNA Polymerase be used for sequencing?
- Does NEB carry modified T7 Polymerase?
- Can T7 DNA Polymerase be used in labeling reactions and partial fill in reactions?
- Are NEB DNA Polymerases supplied with dNTPs?
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- M0274S_L_v1
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- M0274S_L_v1_0111508
- M0274S_L_v1_0111603
- M0274S_L_v1_0111609
- M0274S_L_v1_0111703
- M0274S_L_v1_0111803
- M0274L_v1_10012703
- M0274S_v1_10021765
- M0274L_v1_10021767
- M0274L_v1_10039918
- M0274S_v1_10040917
- M0274L_v1_10057374
- M0274S_v1_10059218
- M0274L_v1_10075649
- M0274L_v1_10081788
- M0274S_v1_10091672
- M0274L_v1_10091094
- M0274S_v1_10107595
- M0274S_v1_10114913
- M0274S_v1_10123097
- M0274L_v1_10131164
- M0274S_v1_10135910
- M0274L_v1_10139934
- M0274L_v1_10152153
- M0274S_v1_10161568
- M0274L_v1_10161623
- M0274S_v1_10163746
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
T7 DNA Polymerase (unmodified)
-
T7 DNA Polymerase Reaction Buffer
-
重组白蛋白, 分子生物学级
-
apohtech MP10042-50T说明书
ApoH-Technologies提出样品预处理溶液,以加强对感染因子的检测。该技术基于载脂蛋白H(ApoH蛋白)与任何种类的生物样品中的微生物结合的能力。一旦与固体支持物结合,ApoH就能够:
- 捕获:与细菌,病毒,真菌,寄生虫结合……
- 浓缩物:来自大量样品的微生物;
- 纯化:去除分析干扰抑制剂。
这种*,快速和可靠的方法与大多数现有检测方法兼容,并提高了诊断灵敏度。
该技术可应用于许多领域:
- 人类健康和动物护理,
- 食品工业,
- 环境安全。
apohtech MP10042-50T说明书
PEPS6-CAPTOVIR KIT
PEPS6-CAPTOVIR试剂盒
说明:
Peps6-CaptoVIR试剂盒可在大多数样品中进行病毒捕获。它包括涂有Peps6的磁珠和一组专门开发的缓冲液,以确保宜捕获病毒。根据试剂盒说明书用缓冲液稀释样品,然后加入珠子。在搅拌下短暂温育后,除去上清液,同时用磁铁(未提供)保持珠子向下。然后将病毒浓缩在珠子上并且没有潜在的抑制剂。可以使用常规检测方法对它们进行分析。该产品的保质期为2年,温度为2-8°C。该产品有20种,50种或100种测试形式。
参考:MP10042-50T
- 数量:50次测试
- 价钱:419€HT
组成:
- 0.5 mL Peps6磁珠(参考:MP20006-0.5ML)
- 3 x 1 mL Buffer TAS 20X(Ref:TP10002-1ML)
- 2 x 1单位添加剂FS 100X(参考:TP10007-1U)
- 1.5 mL溶剂FS(参考:TP10008-1.5ML)
T5 Exonuclease | NEB酶试剂 New England Biolabs
上海金畔生物科技有限公司代理New England Biolabs(NEB)酶试剂全线产品,欢迎访问官网了解更多产品信息和订购。
T5 Exonuclease
This product is discontinued and has been replaced by T5 Exonuclease (NEB #M0663).
- Catalog # M0363 was discontinued on October 02, 2020
- Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease
- Initiates at the 5′ termini of linear or nicked double-stranded DNA
- Cleaves linear or nicked double-stranded DNA in the 5′ to 3′ direction
T5 Exonuclease is ideal for:
- Removal of incomplete ligation products from ligated circular double-stranded DNA
- Degradation of denatured DNA from alkaline-based plasmid purification methods for improved DNA cloning
- Degradation of contaminating linear and nicked DNA in plasmid samples
Storage Temperature: -20°C
产品信息
产品来源
An E. coli strain that carries a plasmid with the T5 phage D15 gene.
- 产品类别:
- Discontinued Products
- 特性和用法
单位定义
One unit is defined as the amount of enzyme required to produce 1 nmol of acid soluble deoxyribonucleotide from double-stranded DNA in 30 minutes at 37°C in a total reaction volume of 50 μl.
反应条件
1X NEBuffer™ 4
Incubate at 37°C1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)贮存溶液
50 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.5 @ 25°C热失活
否
单位活性检测条件
1X NEBuffer 4, 0.15 mM sonicated duplex [3H]-DNA.
- 优势和特性
应用特性
- Degradation of linear ssDNA, dsDNA or nicked plasmid DNA while maintaining supercoiled plasmid DNA.
- Remove denatured DNA isolated during alkaline lysis-based plasmid purification procedure (5).
- Enhance the transfection efficiency of miniprep from plasmid cDNA libraries (6).
- 相关产品
相关产品
- Monarch® Plasmid Miniprep Kit
- Monarch® DNA Gel Extraction Kit
- Monarch® PCR & DNA Cleanup Kit (5 μg)
单独销售的组分
- NEBuffer 4
- 参考文献
- Sayers, J.R. et al. (1991). Nucleic Acids Res. 19, 4127-4132.
- Sayers, J.R. et al. (1990). J. of Biol. Chem. 265, 18311-18317.
- Kaliman, A.V. et al. (1986). FEBS. 195, 61-64.
- Frenkel, G.D. et al. (1971). J. of Biol. Chem. 246, 4839-4847.
- Sayers, J.R. et al. (1996). Analytical Biochem. 241, 186-189.
- Kiss-Toth, E. et al. (2001). Analytical Biochem. 288, 230-232.
操作说明、说明书 & 用法
- 操作说明
- Protocol for T5 Exonuclease (M0363)
- 使用指南
- Activity of DNA Modifying Enzymes in rCutSmart™ Buffer
工具 & 资源
- 选择指南
- Activities of Exonucleases and Non-specific Endonucleases
- Common Applications for Exonucleases and Endonucleases
- Properties of Exonucleases and Non-specific Endonucleases
- Web 工具
- Exo Selector
- 研究摘要
- Choosing the Right Exonuclease (2019)
FAQs & 问题解决指南
- FAQs
- How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
- Will T5 exonuclease cleave supercoiled dsDNA?
- How does T5 Exonuclease differ from Exonuclease III (NEB# M0206)?
- What is the activity of T5 Exonuclease in the NEBuffers?
- Can T5 Exonuclease be heat inactivated?
- Can DNA be blunted using T5 Exonuclease?
- Will T5 Exonuclease work in rCutSmart buffer?
- 实验技巧
Has 5’ to 3’ dsDNA exonuclease activity and ssDNA endonuclease activity.
引用 & 技术文献
- 引用文献
产品引用文献查找工具
Powered by Bioz See more details on Bioz - 专题文章
- The effect of nucleic acid modifications on digestion by DNA exonucleases
质控、安全 & 法规
- 质控分析
每一新批次的 NEB 产品都要经过质控,以满足指定的质量标准,对特定产品的质量标准和每一批次的检测数据可以通过产品说明表格、COA、产品信息卡或者产品手册进行查阅或下载。关于 NEB 产品质控的详细信息,可从此处查阅。
- 产品说明与变更通知
产品说明与变更通知
产品说明表包含产品的储存温度、有效期和规格,这些文件的命名规则如下:[货号]_[规格]_[版本]
- M0363S_L_v1
- M0363S_L_v2
- CoA
CoA 文件包含单个批次产品的储存温度、有效期和质控,这些文件的命名规则如下: [货号]_[规格]_[版本]_[Lot#]
- M0363L_v1_10011608
- M0363L_v1_10024696
- M0363S_v1_10025500
- M0363L_v1_10034706
- M0363S_v1_10039595
- M0363L_v1_10045738
- M0363S_v1_10045737
- M0363L_v1_10055613
- M0363L_v1_10056173
- M0363L_v1_10057996
- M0363S_v1_10057226
- M0363S_v1_10067121
- SDS
以下 SDS 文件可以帮助您安全地使用该产品
-
T5 Exonuclease
-
BSEH-311-96T Human Galectin-1 ELISA KIT
BSEH-311-96T Human Galectin-1 ELISA KIT
¥2700.00
货号:BSEH-311-96T
规格:96 Wells
品牌:biosharp
货号 | BSEH-311-96T |
规格 | 96 Wells |
品牌 | biosharp |
说明书下载 | 点击下载 |