targetingsystems 产品价格表

 Catalog Number质粒Catalog NumberPrice品牌

#0041Profect-P1-lipid based protein delivery reagent50 reactions3200targetingsystems

#0042Profect-P2, a non-lipid based protein delivery reagent50 reactions3200targetingsystems

#0042Profect-P2, a non-lipid based protein delivery reagent150 reactions6720targetingsystems

#0043Profect-P1-lipid based protein delivery reagent150 reactions6720targetingsystems

#0045Profect reporter kit: This kit consists of a positive control protein recombinant Beta galactosidase, 100 ug. Fluorescent Histone and fluorescent IgG) are provided free to assess easily the efficiency of protein delivery to the cytoplasm or nucleus100 ug Beta galactosidase2400targetingsystems

#0060Targefect-siRNA kit100 reacns3520targetingsystems

#0060Targefect-siRNA kit100 reacns3520targetingsystems

#0061Targefect-F-2 plus Virofect enhancer100 reacns4240targetingsystems

#0061Targefect-F-2 plus Virofect enhancer100 reacns4240targetingsystems

#293/FSTargefect-293FS1.2 ml (500 reacns)3040targetingsystems

#C001pCMV-RedFluc plasmid vector- Control plasmid expressing red-emitting firefly luciferase (Luciola Italica) under control of the CMV promoter25 ug4784targetingsystems

#S001psiScreen plasmid vector, a Gaussia luciferase –based  vector to study miRNA expression in cells or gene silencing25 ug6720targetingsystems

(#001)Targefect F-10.6 ml / 100-5002320targetingsystems

(#002)Targefect F-14 X 0.6 ml / 200-10007680targetingsystems

(#003)Targefect F-20.6 ml / 100-5002320targetingsystems

(#004)Targefect F-24 X 0.6 ml / 200-10007680targetingsystems

(#007)Enhancer (Virofect)- Adenovirus derived formulation that dramatically enhances gene transfer when used with F-1 or F-2600 µl1920targetingsystems

(#008)Peptide Enhancer600 ul1920targetingsystems

(#293-01)Targefect-293 (Targefect-F2)1.2 ml (400 reacns)3040targetingsystems

(#293-01)Targefect-293 (Targefect-F2)1.2 ml (400 reacns)3040targetingsystems

(#BAC-01)Targefect-BAC (Targefect F-2 & virofect) For transfection of Bacterial Artificial Chromosomes or large DNA fragments100 reacns4400targetingsystems

(#COS-01)Targefect-COS (Targefect F-2)1.2 ml (400 reacns)2880targetingsystems

(#HEP-01)Targefect-Hepatocyte (Targefect F-1 & virofect) For transfection of primary Human, rat, mouse and chimp hepatocytes200 reacns4400targetingsystems

(#HUVEC-01)Targefect-HUVEC (Targefect F-2 & virofect, peptide enhancer)100 reacns5280targetingsystems

(#MEF-01)Targefect-MEF (Targefect F-2 & virofect) Transfection of mouse embryo fibroblasts1.2ml (400 reacns)4400targetingsystems

(#OST-01)Targefect-Osteoblast (Targefect F-2 virofect)100 reacns4400targetingsystems

(#RAW-01)Targefect-RAW (Targefect F-2 & virofect) Transfection of Raw-264.7 cells and macrophages1.2ml (400 reacns)4400targetingsystems

(#SMC-01)Targefect-SMC (Targefect F-2 & virofect)1.2 ml (100 reacns)4400targetingsystems

5X CLR-015X Cell Lysis reagent100 ml2400targetingsystems

DLAR-1Gaussia-Red Firefly Luciferase1000 assays13600targetingsystems

DLAR-2Green Renilla luciferase -Red Firefly Luciferase1000 assays13600targetingsystems

DLAR-3Cypridina-Red Firefly Luciferase1000 assays13600targetingsystems

DLAR-4Cypridina-Gaussia Luciferase1000 assays13600targetingsystems

DLAR-5Cypridina-Renilla Luciferase1000 assays14400targetingsystems

FLAR-1Firefly Luciferase Assay Reagent1000 assays5600targetingsystems

FLAR-1Firefly luciferase assay reagent1000 assays5600targetingsystems

GAR-1Gaussia Luciferase Assay1000 assays6000targetingsystems

GAR-2Gaussia luciferase assay reagent1000 assays6400targetingsystems

GAR-2 0090AGaussia Luciferase Assay (Stable signal)1000 assays6400targetingsystems

GAR-2BGaussia lLuciferase Assay (Brighter stable version)1000 assays6720targetingsystems

Lenti-CustomAny of the above expressing luciferase under your promoter of interest (subject to availability)Lenti-Custom40000targetingsystems

LiveResponse SystemLVR-0011. (vector)32000targetingsystems

LP-01Lenti plasmid expressing Gaussia luciferase and EGFP, packaging mix, 1000 assays of luciferase assay reagent, The CMV promoter can be excised to replace it with the promoter of choice, Includes pLenti-Basic promoterlexx lentiplasmidpLenti-Basic-Gluc,26400targetingsystems

LP-02Control Lenti plasmid expressing Gaussia luciferase under CMV promoter and EGFP using an IRES, packaging mix, 1000 assays of lcufierase assay reagentpLenti-CMV-GLuc-IRES-GFP26400targetingsystems

LP-05Control Lenti plasmid expressing Red firefly luciferase under control of the CMV promoter along with  EGFP ,pLenti-CMV’RedFluc-IRES-EGFP26400targetingsystems

LP-06Control Lenti plasmid expressing Green Renilla luciferase under CMV promoterpLenti-CMV-GrRenLuc26400targetingsystems

LP-07Lentivirus expressing Gaussia luciferase and GFP, pLenti-CMV-Gluc-IRES-EGFP plasmid, 1000 assays of the Gaussia luciferase assay reagent GAR-1pLenti-CMV-GLuc-IRES-GFP32000targetingsystems

LP-08Lentivirus expressing Red firefly luciferase and GFP, pLenti-CMV-RedFluc-IRES-EGFP plasmid, 1000 assays of the firefly luciferase assay reagent FLAR-1Lenti-CMV-RedFluc-IRES-EGFP32000targetingsystems

LP-12Control Lenti plasmid expressing Green Renilla luciferase under control of CMV promoter and RFP under phosphoglycerokinase promoterpLentiCMV-GrRenLuc-RFP26400targetingsystems

LP-20Lentivirus expressing Green Renilla luciferase under control of CMV promoter and RFP, 1000 assays of the luciferase assay reagent RLAR-1,  and lentiplasmidpLenti-CMV-GrRenLuc-RFP32000targetingsystems

pBasic-BlRenLucPromoterless vector expressing secreted Blue Renilla luciferaseBRL-0034784targetingsystems

pBasic-GLuc-KDELPromoterless expression vector expressing intracellular Gaussia luciferaseGL-K 0024784targetingsystems

pBasic-GrFLucPromoterless vector expressing green Luciola luciferaseGFL-0024784targetingsystems

pBasic-GrRenLucPromoterless vector expressing secreted Green Renilla luciferaseGRL-0034784targetingsystems

pBasic-RedFLcuPromoterless vector expressing red Luciola luciferase (intracellular)FL-0024784targetingsystems

pBasic-RedSecFLucPromoterless vector expressing red firefly luciferaseFL-002S4784targetingsystems

pBasic-VargLucPromoterless vector expressing Cypridina LuciferaseVL-0024784targetingsystems

pCMV-BlRenLucExpresses secreted Blue Renillla luciferase under CMV promoterBRL-0014784targetingsystems

pCMV-GLucExpresses native Gaussia luciferase under CMV promoterGL-0014784targetingsystems

pCMV-GLuc-KDELExpresses intracellular Gaussia luciferease under control of CMV promtoter due to presence of KDEL (endoplasmic reticulum retention sequence)GL-K 0014784targetingsystems

pCMV-GrFLucExpresses intracellular firefly luciferase (Luciola Italica)GFL-0014784targetingsystems

pCMV-GrRenLucExpresses secreted Green Renillla luciferase under CMV promoterGRL-0014784targetingsystems

pCMV-RedFlucExpresses red firefly luciferase (intracellular) under control of CMV promoterFL-0014784targetingsystems

pCMV-RedSecFLucExpresses a secreted form of the red firefly luciferase under CMV promtoerFL-001S4784targetingsystems

pCMV-VargLucExpresses Cyprdina (Vargula) luciferase under control of the CMV promoterVL-0014784targetingsystems

pGLuc-BasicPromoterless vector expressing Gaussia luciferaseGL-0024784targetingsystems

plenti-GLuc-IRES-EGFPLenti vector expressing Gaussia luciferase under control of CMV promoter, co-expressing EGFP using an IRES.GL-GFP9600targetingsystems

RLAR-1Renilla Luciferase Assay reagent1000 assays6400targetingsystems

TLAR-1Cypridina-Green Renilla-Red Firefly Luciferse Assay Reagent1000 assays16000targetingsystems

TLAR-2Cypridina Luciferase Gaussia Luciferase, red Firefly Luciferase Assay reagent1000 assays16000targetingsystems

VLAR-1Cypridina (Vargula) Luciferase Assay Reagent1000 assays6400targetingsystems

VLAR-2Cypridina Luciferse Assay reagent (more stable version)1000 assays7040targetingsystems

 

targetingsystems品牌简介

品牌简介

Targeting Systems 于 1996 年 5 月*在 NIH 的 SBIR 第一阶段资助的帮助下启动。该公司在成立的第一年就推出了第一条产品线 Targefect™。该公司最初是一个人运营,现在提供 100 多种基于非常多样化和强大的 IP 组合的产品 Targeting Systems 已应用其在生物发光方面的专业知识来应对药物发现、基因表达和干细胞研究方面的挑战。自成立以来,该公司剥离了 Pluristem Innovations(2007 年,开始将我们的多种技术应用于干细胞研究和治疗)并帮助在印度成立了一家公司(Shakti Biosystems)

生物发光:

荧光素酶报告基因和检测系统
最佳和最亮的单、双、三和五倍报告系统,涵盖整个光谱,专为高通量筛选量身定制。

  • Gaussia 萤光素酶(λmax 482 nm) –
    我们是第一个为 Gaussia 萤光素酶开发哺乳动物表达载体和 HTS 兼容检测系统的公司,Gaussia 萤光素酶是已知的最明亮的分泌型萤光素酶。(许可形式 Prolume Inc,亚利桑那州)。

  • Cyrpridina luciferase (λmax 467 nm) –
    一种明亮的分泌型荧光素酶,使用*的荧光素 Cypridina luciferin 作为底物

  • Green Renilla 萤光素酶(λmax 527 nm) –
    最亮的 Renilla 萤光素酶,由随机诱变和合理设计相结合产生,并设计为分泌型。

  • Blue Renilla Luciferase (λmax 467 nm) –
    通过诱变产生的可分泌版本。

  • 来自意大利萤火虫 Luciola Italica 的发红光 萤火虫荧光素酶(λmax 617 nm)和发绿光萤火虫荧光素酶 –
    耐热突变体,比原生萤火虫 亮 1000 倍。也可以秘密形式提供。

  • 来自 Luciola Italica 的 绿色荧光素酶(λmax 550 nm)。


高通量筛选:

用于 HTS 的多重荧光素酶检测
我们是第一个开发基于单一溶液的三重荧光素酶报告基因检测 -可以使用一种检测溶液通过光谱分辨率使用光度计中的适当过滤器测量同一样品中 3 种不同的分泌荧光素酶活性。节省筛选时间,提高检测灵敏度(更亮的信号)并与 HTS 兼容。细胞在下游应用中保持活力。

Lenti-Luciferase 产品线 LentiPower™ 和 LentiGlo™

利用慢病毒技术的力量,结合我们的超灵敏新型荧光素酶报告基因组,创建包含您感兴趣的启动子(LentiPower= 或创建稳定转染的细胞,使用 LentiGlo 表达我们*的荧光素酶(如果需要,带有 FP)的表达荧光素酶的慢病毒。即使是最困难细胞类型的转导效率几乎为 100%。应用包括体内成像和植入肿瘤或干细胞的跟踪(请参阅我们网站https://targetingsystems.net/drug-discovery 上药物发现链接中的图表和表格 。 php



基因传递 – Targefect™ / Virofect™ 转染试剂:

  • Targefect F-1 和 Targefect F-2 –
    提供基于脂质 (Targefect F'1) 和非脂质聚合物 (Targefect F-2) 的试剂。超过 100 次在期刊上的引用记录了他们的表现

  • Virofect – 一种*的腺病毒衍生增强剂配方(Targetign Systems 拥有的技术)
    – 基因转移提高了 10-1000 倍。
    – 在许多原代细胞中的转染效果甚至优于 Amaxa 系统或 Neon 系统(均使用电穿孔并需要对细胞进行胰蛋白酶消化),同时毒性低。

  • 细胞特异性转染试剂 –
    为许多常用细胞系和原代细胞提供优化方案和转染试剂盒

  • siRNA 和 microRNA 转染 –
    Targefect siRNA 试剂盒或 Targefect F-2 和 Virofect 的组合可以有效地将 siRNA、microRNA 或 RNAi 与质粒 DNA 的共同递送有效地传递到最难转移的细胞中。

基因传递(体外和体内)和基于荧光素酶的用于体外和体内筛选 siRNA 和 microRNA 的技术

蛋白质输送:

Profect-P1 和 Profect-P2:
*的蛋白质递送试剂,已证明可用于多种应用,例如将人类干细胞分化为特定谱系,以及将多种功能活性蛋白质递送至巨噬细胞和原代细胞。请参阅我们的 ppt 演示文稿以了解性能和认可链接的概述

蛋白质表达:

使用专门的目标制剂 (Targefect-293F) 和转染方案,以大大降低的价格高效转染自由式 293 悬浮细胞。

干细胞 :

  • 干细胞转染:
    使用 Virofect 和 Targefect 的组合将基因有效转移到胚胎和人类成人间充质干细胞中

  • 干细胞分化:
    使用 Profect 通过位点特异性递送特定蛋白质将人类干细胞分化为特定谱系。

  • 干细胞
    扩增、生长和存活:我们的 LentiGlo 技术已被证明对研究体外和体内干细胞的生长、存活和分化非常有用。

 

 

targetingsystems基因沉默产品简介

targetingsystems基因沉默产品简介

使用基于 Gaussia 荧光素酶的 psiScreen 系统可以轻松筛选 siRNA。实时高通量筛选 siRNA 的理想选择。想象一下没有蛋白质印迹或实时 PCR!





不同 siRNA 沉默 p53 基因的有效性





基因沉默变得简单快捷:使用 targefect siRNA 试剂盒高效转染 siRNA。

Catalog # Capacity Price

#S00125 µg psiScreen  plus GAR-1 reagent   $420

#C001, (100 assays)pCMV-Fluc (25 µg)$200

 

targetingsystems LentiGloTM说明书

targetingsystems LentiGloTM说明书
预包装慢病毒和慢质粒载体
定制慢病毒生产和生物发光细胞系服务
产品供应:
•表达已知最亮、最小的Gaussia荧光素酶的预制慢病毒和慢质粒载体
荧光素酶和荧光蛋白。
•慢病毒载体,表达意大利绿蝇(Luciola Italica)的非常亮的红色萤火虫荧光素酶
•表达细胞内绿色发光肾素荧光素酶的慢病毒载体(比天然载体亮35倍)
Renilla荧光素酶,最大发射波长为527 nm)
•荧光素酶与荧光蛋白共表达的慢病毒载体(选择EGFP、mVermilionn
或者mScarlet。mScarlet和mVermilion(均为单体)是非常亮的红色荧光蛋白
高斯荧光素酶和红色萤火虫及绿色雷尼拉荧光素酶的优点:
•意大利萤火虫Luciola Italica的一种亮红色发光变种,最大发射波长为617 nm,具有优势
具有明亮、更稳定的生物发光信号,对深层组织成像非常有用。超过50名癌症患者
细胞系已稳定转导并在体内测试
•高斯荧光素酶是已知最小、最亮的荧光素酶,比萤火虫或萤火虫的亮度高1000倍
雷尼拉荧光素酶。提高体内成像应用的灵敏度,使用腔烯特拉嗪作为底物。
•Gaussia荧光素酶由哺乳动物细胞主动分泌,因此有可能对细胞的生长进行定量
通过测定血液中荧光素酶活性来植入细胞
•我们的绿色发光Renilla荧光素酶报告器比天然Renilla荧光素酶报告器亮35倍
•所有LentiGlo产品都具有高滴度慢病毒(自失活)
•我们所有的现成慢病毒都是SIN(自失活)载体,因此一旦病毒整合到基因组中
在被转导的细胞中,它被灭活,因此不能再复制。
•表达塞浦路斯荧光素酶的慢病毒可用作定制慢病毒载体

LP-30    Lenti-UBC-RedFluct2A-Puro    

LP-31    Lenti-UBC-RedFluct2A-EGFP

LP-42    Lenti-CMV-GLuc-T2ARedFluc-T2A-Puro

LP-43    Lenti-CMV-GLuc-T2AEGFP