标签归档：thermostable

Thermostable RNase H |NEB酶试剂 New England Biolabs

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Thermostable RNase H

Thermostable RNase H specifically recognizes and cleaves the phosphodiester bonds of an RNA strand in an RNA-DNA hybrid while leaving the DNA strand intact. This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures.

  • Recombinant enzyme supplied with 10X Reaction Buffer
  • Does not digest single or double-stranded DNA
  • Active and stable above 37°C over a broad temperature range, with activity increasing with temperature
  • Requires magnesium chloride for activity
  • Inactivated with proteinase K treatment or addition of excess EDTA

Thermostable RNase H |

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M0523S 5,000 units/ml 250 units ¥1,719.00 有 无 有 有 有
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库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0523S 5,000 units/ml 250 units ¥1,719.00 有 无 有 有 有

Thermostable FEN1 |NEB酶试剂 New England Biolabs

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上海金畔生物科技有限公司代理New England Biolabs（NEB）酶试剂全线产品，欢迎访问官网了解更多产品信息和订购。

Thermostable FEN1

Thermostable FEN1 |

  • Thermostable DNA and RNA endonuclease
  • Catalyzes the cleavage of 5′ DNA and RNA flaps from branched double stranded DNA substrates, creating a 5′ phosphate terminus
  • FEN 1 products can be ligated by DNA ligase to create double stranded DNA
  • Heat inactivation not possible
  • This is an Enzyme for Innovation. EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities

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Thermostable FEN1 |

What are endonucleases and their applications?

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货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0645S 32,000 units/ml 1,600 units ¥909.00 有 无 无 有 有
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库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0645S 32,000 units/ml 1,600 units ¥909.00 有 无 无 有 有

Thermostable OGG |NEB酶试剂 New England Biolabs

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Thermostable OGG

Thermostable OGG |

  • Thermostable oxoguanine glycosylase
  • Bifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities
  • N-glycosylase activity releases 8-oxo-7,8-dihydroguanine (8-oxoG), generating an AP site. The AP-lyase activity cleaves 3′ to the AP site leaving a 5′ phosphate and a 3′-phospho-α, β-unsaturated aldehyde.
 

Thermostable OGG |

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货号 浓度 规格 目录价 北京 上海 广州 成都 苏州
M0464S 8,000 units/ml 500 units ¥899.00 无 无 无 无 无
如何订购
Please enter a quantity for at least one size

库存 货号 浓度 规格 目录价 北京 上海 广州 成都 苏州 M0464S 8,000 units/ml 500 units ¥899.00 无 无 无 无 无

Thermostable USER® III Enzyme |NEB酶试剂 New England Biolabs

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Thermostable USER® III Enzyme

Thermostable USER (Uracil-Specific Excision Reagent) III Enzyme generates a single nucleotide gap at the location of a uracil residue. It is active between 50-75°C (optimal activity observed at 65°C).

Figure 1: Thermostable USER III Enzyme

Thermostable USER® III Enzyme |

Afu Uracil DNA glycosylase (UDG) catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease IV breaks the phosphodiester backbone at the 3′ and 5′ sides of the abasic site so that base-free deoxyribose is released.